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Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice.
J Exp Bot. 2015 Feb; 66(3):973-87.JE

Abstract

Plant leaf senescence and defence responses are important biological processes, but the molecular mechanisms involved are not well understood. This study identified a new rice mutant, spotted leaf 29 (spl29). The SPL29 gene was identified by map-based cloning, and SPL29 was confirmed as UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) by enzymatic analysis. The mutant spl29 lacks UAP activity. The biological phenotypes for which UAP is responsible have not previously been reported in plants. The spl29 mutant displayed early leaf senescence, confirmed by chlorophyll loss and photosystem II decline as physiological indicators, chloroplast degradation as a cellular characteristic, and both upregulation of senescence transcription factors and senescence-associated genes, and downregulation of photosynthesis-related genes, as molecular evidence. Defence responses were induced in the spl29 mutant, shown by enhanced resistance to bacterial blight inoculation and upregulation of defence response genes. Reactive oxygen species, including O2 (-) and H2O2, accumulated in spl29 plants; there was also increased malondialdehyde content. Enhanced superoxide dismutase activity combined with normal catalase activity in spl29 could be responsible for H2O2 accumulation. The plant hormones jasmonic acid and abscisic acid also accumulated in spl29 plants. ROS and plant hormones probably play important roles in early leaf senescence and defence responses in the spl29 mutant. Based on these findings, it is suggested that UAP1 is involved in regulating leaf senescence and defence responses in rice.

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Authors+Show Affiliations

Wang Z
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.
Wang Y
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.
Hong X
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.
Hu D
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.
Liu C
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China.
Yang J
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.
Li Y
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.
Huang Y
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Hubei, 430072, China.
Feng Y
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Hubei, 430072, China.
Gong H
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.
Li Y
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.
Fang G
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China.
Tang H
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China State Key Laboratory of Genetic Engineering, Metabolomics Laboratory, School of Life Sciences, Fudan University, Shanghai 200433, China.
Li Y
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Hubei 430072, China lysh2001@whu.edu.cn.

MeSH

MutationNucleotidyltransferasesOryzaPhylogenyPlant ImmunityPlant LeavesPlant Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25399020

Citation

Wang, Zhaohai, et al. "Functional Inactivation of UDP-N-acetylglucosamine Pyrophosphorylase 1 (UAP1) Induces Early Leaf Senescence and Defence Responses in Rice." Journal of Experimental Botany, vol. 66, no. 3, 2015, pp. 973-87.
Wang Z, Wang Y, Hong X, et al. Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice. J Exp Bot. 2015;66(3):973-87.
Wang, Z., Wang, Y., Hong, X., Hu, D., Liu, C., Yang, J., Li, Y., Huang, Y., Feng, Y., Gong, H., Li, Y., Fang, G., Tang, H., & Li, Y. (2015). Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice. Journal of Experimental Botany, 66(3), 973-87. https://doi.org/10.1093/jxb/eru456
Wang Z, et al. Functional Inactivation of UDP-N-acetylglucosamine Pyrophosphorylase 1 (UAP1) Induces Early Leaf Senescence and Defence Responses in Rice. J Exp Bot. 2015;66(3):973-87. PubMed PMID: 25399020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice. AU - Wang,Zhaohai, AU - Wang,Ya, AU - Hong,Xiao, AU - Hu,Daoheng, AU - Liu,Caixiang, AU - Yang,Jing, AU - Li,Yang, AU - Huang,Yunqing, AU - Feng,Yuqi, AU - Gong,Hanyu, AU - Li,Yang, AU - Fang,Gen, AU - Tang,Huiru, AU - Li,Yangsheng, Y1 - 2014/11/15/ PY - 2014/11/16/entrez PY - 2014/11/16/pubmed PY - 2016/3/10/medline KW - Defence responses KW - SPL29 KW - UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1). KW - leaf senescence KW - reactive oxygen species (ROS) KW - rice (Oryza sativa) SP - 973 EP - 87 JF - Journal of experimental botany JO - J Exp Bot VL - 66 IS - 3 N2 - Plant leaf senescence and defence responses are important biological processes, but the molecular mechanisms involved are not well understood. This study identified a new rice mutant, spotted leaf 29 (spl29). The SPL29 gene was identified by map-based cloning, and SPL29 was confirmed as UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) by enzymatic analysis. The mutant spl29 lacks UAP activity. The biological phenotypes for which UAP is responsible have not previously been reported in plants. The spl29 mutant displayed early leaf senescence, confirmed by chlorophyll loss and photosystem II decline as physiological indicators, chloroplast degradation as a cellular characteristic, and both upregulation of senescence transcription factors and senescence-associated genes, and downregulation of photosynthesis-related genes, as molecular evidence. Defence responses were induced in the spl29 mutant, shown by enhanced resistance to bacterial blight inoculation and upregulation of defence response genes. Reactive oxygen species, including O2 (-) and H2O2, accumulated in spl29 plants; there was also increased malondialdehyde content. Enhanced superoxide dismutase activity combined with normal catalase activity in spl29 could be responsible for H2O2 accumulation. The plant hormones jasmonic acid and abscisic acid also accumulated in spl29 plants. ROS and plant hormones probably play important roles in early leaf senescence and defence responses in the spl29 mutant. Based on these findings, it is suggested that UAP1 is involved in regulating leaf senescence and defence responses in rice. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/25399020/Functional_inactivation_of_UDP_N_acetylglucosamine_pyrophosphorylase_1__UAP1__induces_early_leaf_senescence_and_defence_responses_in_rice_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/eru456 DB - PRIME DP - Unbound Medicine ER -