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[Expression and significance of Wnt/β-catenin signaling pathway in vitro natural degenerationmodel of endplate chondrocytes in rats].
Zhonghua Yi Xue Za Zhi 2014; 94(31):2464-7ZY

Abstract

OBJECTIVE

To explore the expression and significance of Wnt/β-catenin signaling pathway in the natural degeneration of endplate chondrocytes in rats.

METHODS

Endplate chondrocytes extracted from lumbar vertebrae were divided into control (P2 cell), naturally passaged (P5 cell) and wnt signaling pathway inhibition groups. The morphology of endplate chondrocytes was observed by inverted microscope. Hematoxylin and eosin (HE) and toluidine blue stains were used to identify their phenotypes. Type II collagen marker, SOX-9 and aggrecan genes were detected by reverse transcription-polymerase chain reaction (RT-PCR) to verify the degeneration model. Based on this model, the changes of β-catenin were detected by RT-PCR and Western blot. Laser confocal microscopy was used to detect the expression and localization of β-catenin within endplate chondrocytes.

RESULTS

With natural passaging, endplate cartilage cells appeared spindle-shaped and gradually lost chondrocytic phenotypes. The levels of type II collagen (P5/P2 = 0.11, P = 0.003 9), SOX-9 (P5/P2 = 0.58, P = 0.016 8) and aggrecan (P5/P2 = 0.32, P = 0.004 6) significantly reduced; β-catenin (P5/P2 = 1.62, P = 0.008 2) significantly increased. β-catenin was down-regulated (XAV-939/P5 = 0.23, P = 0.001 7) in inhibition group. And type II collagen (XAV-939/P5 = 2.60, P = 0.018 0), SOX-9 (XAV-939/P5 = 1.47, P = 0.038 2) and aggrecan (XAV-939/P5 = 2.56, P = 0.004 1) significantly increased. β-catenin had a higher expression and obviously entered into nuclear transcription in P5 generation and decreased in inhibition group.

CONCLUSION

β-catenin plays an important role in the in vitro degeneration of endplate chondrocytes. There are great potentials for protecting endplate cartilage degeneration by regulating the Wnt/β-catenin signaling pathway.

Authors+Show Affiliations

Department of Orthopedic Surgery, Yijishan Hosptial, Wannan Medical College, Wuhu 241001, China.Email: xuhg@medmail.com.cn.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

25400058

Citation

Zheng, Quan, et al. "[Expression and Significance of Wnt/β-catenin Signaling Pathway in Vitro Natural Degenerationmodel of Endplate Chondrocytes in Rats]." Zhonghua Yi Xue Za Zhi, vol. 94, no. 31, 2014, pp. 2464-7.
Zheng Q, Xu H, Zhang X, et al. [Expression and significance of Wnt/β-catenin signaling pathway in vitro natural degenerationmodel of endplate chondrocytes in rats]. Zhonghua Yi Xue Za Zhi. 2014;94(31):2464-7.
Zheng, Q., Xu, H., Zhang, X., Wang, H., Wang, J., Wang, C., ... Zhao, Q. (2014). [Expression and significance of Wnt/β-catenin signaling pathway in vitro natural degenerationmodel of endplate chondrocytes in rats]. Zhonghua Yi Xue Za Zhi, 94(31), pp. 2464-7.
Zheng Q, et al. [Expression and Significance of Wnt/β-catenin Signaling Pathway in Vitro Natural Degenerationmodel of Endplate Chondrocytes in Rats]. Zhonghua Yi Xue Za Zhi. 2014 Aug 19;94(31):2464-7. PubMed PMID: 25400058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression and significance of Wnt/β-catenin signaling pathway in vitro natural degenerationmodel of endplate chondrocytes in rats]. AU - Zheng,Quan, AU - Xu,Hongguang, AU - Zhang,Xiaoling, AU - Wang,Hong, AU - Wang,Jing, AU - Wang,Chuandong, AU - Song,Junxing, AU - Xiong,Shouliang, AU - Zhao,Quanlai, PY - 2014/11/18/entrez PY - 2014/11/18/pubmed PY - 2015/9/9/medline SP - 2464 EP - 7 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 94 IS - 31 N2 - OBJECTIVE: To explore the expression and significance of Wnt/β-catenin signaling pathway in the natural degeneration of endplate chondrocytes in rats. METHODS: Endplate chondrocytes extracted from lumbar vertebrae were divided into control (P2 cell), naturally passaged (P5 cell) and wnt signaling pathway inhibition groups. The morphology of endplate chondrocytes was observed by inverted microscope. Hematoxylin and eosin (HE) and toluidine blue stains were used to identify their phenotypes. Type II collagen marker, SOX-9 and aggrecan genes were detected by reverse transcription-polymerase chain reaction (RT-PCR) to verify the degeneration model. Based on this model, the changes of β-catenin were detected by RT-PCR and Western blot. Laser confocal microscopy was used to detect the expression and localization of β-catenin within endplate chondrocytes. RESULTS: With natural passaging, endplate cartilage cells appeared spindle-shaped and gradually lost chondrocytic phenotypes. The levels of type II collagen (P5/P2 = 0.11, P = 0.003 9), SOX-9 (P5/P2 = 0.58, P = 0.016 8) and aggrecan (P5/P2 = 0.32, P = 0.004 6) significantly reduced; β-catenin (P5/P2 = 1.62, P = 0.008 2) significantly increased. β-catenin was down-regulated (XAV-939/P5 = 0.23, P = 0.001 7) in inhibition group. And type II collagen (XAV-939/P5 = 2.60, P = 0.018 0), SOX-9 (XAV-939/P5 = 1.47, P = 0.038 2) and aggrecan (XAV-939/P5 = 2.56, P = 0.004 1) significantly increased. β-catenin had a higher expression and obviously entered into nuclear transcription in P5 generation and decreased in inhibition group. CONCLUSION: β-catenin plays an important role in the in vitro degeneration of endplate chondrocytes. There are great potentials for protecting endplate cartilage degeneration by regulating the Wnt/β-catenin signaling pathway. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/25400058/[Expression_and_significance_of_Wnt/β_catenin_signaling_pathway_in_vitro_natural_degenerationmodel_of_endplate_chondrocytes_in_rats]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0376-2491&year=2014&vol=94&issue=31&fpage=2464 DB - PRIME DP - Unbound Medicine ER -