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The C. elegans dosage compensation complex mediates interphase X chromosome compaction.
Epigenetics Chromatin 2014; 7(1):31EC

Abstract

BACKGROUND

Dosage compensation is a specialized gene regulatory mechanism which equalizes X-linked gene expression between sexes. In Caenorhabditis elegans, dosage compensation is achieved by the activity of the dosage compensation complex (DCC). The DCC localizes to both X chromosomes in hermaphrodites to downregulate gene expression by half. The DCC contains a subcomplex (condensin I(DC)) similar to the evolutionarily conserved condensin complexes which play fundamental roles in chromosome dynamics during mitosis and meiosis. Therefore, mechanisms related to mitotic chromosome condensation have been long hypothesized to mediate dosage compensation. However experimental evidence was lacking.

RESULTS

Using 3D FISH microscopy to measure the volumes of X and chromosome I territories and to measure distances between individual loci, we show that hermaphrodite worms deficient in DCC proteins have enlarged interphase X chromosomes when compared to wild type. By contrast, chromosome I is unaffected. Interestingly, hermaphrodite worms depleted of condensin I or II show no phenotype. Therefore X chromosome compaction is specific to condensin I(DC). In addition, we show that SET-1, SET-4, and SIR-2.1, histone modifiers whose activity is regulated by the DCC, need to be present for the compaction of the X chromosome territory.

CONCLUSION

These results support the idea that condensin I(DC), and the histone modifications regulated by the DCC, mediate interphase X chromosome compaction. Our results link condensin-mediated chromosome compaction, an activity connected to mitotic chromosome condensation, to chromosome-wide repression of gene expression in interphase.

Authors+Show Affiliations

Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109 Michigan.Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109 Michigan.Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109 Michigan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25400696

Citation

Lau, Alyssa C., et al. "The C. Elegans Dosage Compensation Complex Mediates Interphase X Chromosome Compaction." Epigenetics & Chromatin, vol. 7, no. 1, 2014, p. 31.
Lau AC, Nabeshima K, Csankovszki G. The C. elegans dosage compensation complex mediates interphase X chromosome compaction. Epigenetics Chromatin. 2014;7(1):31.
Lau, A. C., Nabeshima, K., & Csankovszki, G. (2014). The C. elegans dosage compensation complex mediates interphase X chromosome compaction. Epigenetics & Chromatin, 7(1), p. 31. doi:10.1186/1756-8935-7-31.
Lau AC, Nabeshima K, Csankovszki G. The C. Elegans Dosage Compensation Complex Mediates Interphase X Chromosome Compaction. Epigenetics Chromatin. 2014;7(1):31. PubMed PMID: 25400696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The C. elegans dosage compensation complex mediates interphase X chromosome compaction. AU - Lau,Alyssa C, AU - Nabeshima,Kentaro, AU - Csankovszki,Györgyi, Y1 - 2014/10/27/ PY - 2014/07/18/received PY - 2014/10/06/accepted PY - 2014/11/18/entrez PY - 2014/11/18/pubmed PY - 2014/11/18/medline KW - Caenorhabditis elegans KW - Chromatin KW - Chromosome condensation KW - Condensin KW - Dosage compensation KW - Epigenetics KW - Gene expression KW - Interphase chromosome SP - 31 EP - 31 JF - Epigenetics & chromatin JO - Epigenetics Chromatin VL - 7 IS - 1 N2 - BACKGROUND: Dosage compensation is a specialized gene regulatory mechanism which equalizes X-linked gene expression between sexes. In Caenorhabditis elegans, dosage compensation is achieved by the activity of the dosage compensation complex (DCC). The DCC localizes to both X chromosomes in hermaphrodites to downregulate gene expression by half. The DCC contains a subcomplex (condensin I(DC)) similar to the evolutionarily conserved condensin complexes which play fundamental roles in chromosome dynamics during mitosis and meiosis. Therefore, mechanisms related to mitotic chromosome condensation have been long hypothesized to mediate dosage compensation. However experimental evidence was lacking. RESULTS: Using 3D FISH microscopy to measure the volumes of X and chromosome I territories and to measure distances between individual loci, we show that hermaphrodite worms deficient in DCC proteins have enlarged interphase X chromosomes when compared to wild type. By contrast, chromosome I is unaffected. Interestingly, hermaphrodite worms depleted of condensin I or II show no phenotype. Therefore X chromosome compaction is specific to condensin I(DC). In addition, we show that SET-1, SET-4, and SIR-2.1, histone modifiers whose activity is regulated by the DCC, need to be present for the compaction of the X chromosome territory. CONCLUSION: These results support the idea that condensin I(DC), and the histone modifications regulated by the DCC, mediate interphase X chromosome compaction. Our results link condensin-mediated chromosome compaction, an activity connected to mitotic chromosome condensation, to chromosome-wide repression of gene expression in interphase. SN - 1756-8935 UR - https://www.unboundmedicine.com/medline/citation/25400696/The_C__elegans_dosage_compensation_complex_mediates_interphase_X_chromosome_compaction_ L2 - https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/1756-8935-7-31 DB - PRIME DP - Unbound Medicine ER -