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Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data.
PLoS One. 2014; 9(11):e113346.Plos

Abstract

The prevention of non-indigenous aquatic invasive species spreading into new areas is a goal of many resource managers. New techniques have been developed to survey for species that are difficult to capture with conventional gears that involve the detection of their DNA in water samples (eDNA). This technique is currently used to track the invasion of bigheaded carps (silver carp and bighead carp; Hypophthalmichthys molitrix and H. nobilis) in the Chicago Area Waterway System and Upper Mississippi River. In both systems DNA has been detected from silver carp without the capture of a live fish, which has led to some uncertainty about the source of the DNA. The potential contribution to eDNA by vectors and fomites has not been explored. Because barges move from areas with a high abundance of bigheaded carps to areas monitored for the potential presence of silver carp, we used juvenile silver carp to simulate the barge transport of dead bigheaded carp carcasses, slime residue, and predator feces to determine the potential of these sources to supply DNA to uninhabited waters where it could be detected and misinterpreted as indicative of the presence of live bigheaded carp. Our results indicate that all three vectors are feasible sources of detectable eDNA for at least one month after their deposition. This suggests that current monitoring programs must consider alternative vectors of DNA in the environment and consider alternative strategies to minimize the detection of DNA not directly released from live bigheaded carps.

Authors+Show Affiliations

IAP Worldwide Services Inc., Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin, United States of America; United States Geological Survey, Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin, United States of America.United States Geological Survey, Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin, United States of America.United States Geological Survey, Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin, United States of America.United States Geological Survey, Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin, United States of America.United States Geological Survey, Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin, United States of America.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

25402206

Citation

Merkes, Christopher M., et al. "Persistence of DNA in Carcasses, Slime and Avian Feces May Affect Interpretation of Environmental DNA Data." PloS One, vol. 9, no. 11, 2014, pp. e113346.
Merkes CM, McCalla SG, Jensen NR, et al. Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data. PLoS ONE. 2014;9(11):e113346.
Merkes, C. M., McCalla, S. G., Jensen, N. R., Gaikowski, M. P., & Amberg, J. J. (2014). Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data. PloS One, 9(11), e113346. https://doi.org/10.1371/journal.pone.0113346
Merkes CM, et al. Persistence of DNA in Carcasses, Slime and Avian Feces May Affect Interpretation of Environmental DNA Data. PLoS ONE. 2014;9(11):e113346. PubMed PMID: 25402206.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data. AU - Merkes,Christopher M, AU - McCalla,S Grace, AU - Jensen,Nathan R, AU - Gaikowski,Mark P, AU - Amberg,Jon J, Y1 - 2014/11/17/ PY - 2014/07/03/received PY - 2014/10/22/accepted PY - 2014/11/18/entrez PY - 2014/11/18/pubmed PY - 2016/2/2/medline SP - e113346 EP - e113346 JF - PloS one JO - PLoS ONE VL - 9 IS - 11 N2 - The prevention of non-indigenous aquatic invasive species spreading into new areas is a goal of many resource managers. New techniques have been developed to survey for species that are difficult to capture with conventional gears that involve the detection of their DNA in water samples (eDNA). This technique is currently used to track the invasion of bigheaded carps (silver carp and bighead carp; Hypophthalmichthys molitrix and H. nobilis) in the Chicago Area Waterway System and Upper Mississippi River. In both systems DNA has been detected from silver carp without the capture of a live fish, which has led to some uncertainty about the source of the DNA. The potential contribution to eDNA by vectors and fomites has not been explored. Because barges move from areas with a high abundance of bigheaded carps to areas monitored for the potential presence of silver carp, we used juvenile silver carp to simulate the barge transport of dead bigheaded carp carcasses, slime residue, and predator feces to determine the potential of these sources to supply DNA to uninhabited waters where it could be detected and misinterpreted as indicative of the presence of live bigheaded carp. Our results indicate that all three vectors are feasible sources of detectable eDNA for at least one month after their deposition. This suggests that current monitoring programs must consider alternative vectors of DNA in the environment and consider alternative strategies to minimize the detection of DNA not directly released from live bigheaded carps. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25402206/Persistence_of_DNA_in_carcasses_slime_and_avian_feces_may_affect_interpretation_of_environmental_DNA_data_ L2 - http://dx.plos.org/10.1371/journal.pone.0113346 DB - PRIME DP - Unbound Medicine ER -