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Increased expression of TRPV1 in the trigeminal ganglion is involved in orofacial pain during experimental tooth movement in rats.
Eur J Oral Sci. 2015 Feb; 123(1):17-23.EJ

Abstract

To investigate whether transient receptor potential vanilloid type 1 (TRPV1) is involved in pain induced by experimental tooth movement, experiments were performed in male Sprague-Dawley rats weighing 200-250 g. Directed face-grooming behavior was used to evaluate nocifensive behavior in rats during experimental tooth movement. The distribution of TRPV1 in the trigeminal ganglion (TG) was evaluated by immunohistochemistry, and its expression was detected by western blotting at several time points following the application of various magnitudes of force during tooth movement. Immunohistochemical analysis revealed that TRPV1 was expressed in TG, and its expression was increased after experimental tooth movement. Western blot results also showed that experimental tooth movement led to a statistically significant increase in expression of TRPV1 protein in TG. Meanwhile, the time spent on directed face-grooming peaked on day 1 and thereafter showed a gradual decrease. In addition, both the change in TRPV1 expression in the TG and directed face-grooming behavior were modulated in a force-dependent manner and in concert with initial orthodontic pain responses. Our results reveal that TRPV1 expression is modulated by experimental tooth movement and is involved in tooth-movement pain.

Authors+Show Affiliations

Department of Orthodontics, the Affiliated Stomatological Hospital of Xi'an Jiaotong University, Xian, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25407056

Citation

Qiao, Hu, et al. "Increased Expression of TRPV1 in the Trigeminal Ganglion Is Involved in Orofacial Pain During Experimental Tooth Movement in Rats." European Journal of Oral Sciences, vol. 123, no. 1, 2015, pp. 17-23.
Qiao H, Gao Y, Zhang C, et al. Increased expression of TRPV1 in the trigeminal ganglion is involved in orofacial pain during experimental tooth movement in rats. Eur J Oral Sci. 2015;123(1):17-23.
Qiao, H., Gao, Y., Zhang, C., & Zhou, H. (2015). Increased expression of TRPV1 in the trigeminal ganglion is involved in orofacial pain during experimental tooth movement in rats. European Journal of Oral Sciences, 123(1), 17-23. https://doi.org/10.1111/eos.12158
Qiao H, et al. Increased Expression of TRPV1 in the Trigeminal Ganglion Is Involved in Orofacial Pain During Experimental Tooth Movement in Rats. Eur J Oral Sci. 2015;123(1):17-23. PubMed PMID: 25407056.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased expression of TRPV1 in the trigeminal ganglion is involved in orofacial pain during experimental tooth movement in rats. AU - Qiao,Hu, AU - Gao,YuNan, AU - Zhang,Caidi, AU - Zhou,Hong, Y1 - 2014/11/19/ PY - 2014/10/15/accepted PY - 2014/11/20/entrez PY - 2014/11/20/pubmed PY - 2016/2/18/medline KW - directed face-grooming KW - experimental tooth movement KW - pain KW - transient receptor potential vanilloid type 1 KW - trigeminal ganglion SP - 17 EP - 23 JF - European journal of oral sciences JO - Eur. J. Oral Sci. VL - 123 IS - 1 N2 - To investigate whether transient receptor potential vanilloid type 1 (TRPV1) is involved in pain induced by experimental tooth movement, experiments were performed in male Sprague-Dawley rats weighing 200-250 g. Directed face-grooming behavior was used to evaluate nocifensive behavior in rats during experimental tooth movement. The distribution of TRPV1 in the trigeminal ganglion (TG) was evaluated by immunohistochemistry, and its expression was detected by western blotting at several time points following the application of various magnitudes of force during tooth movement. Immunohistochemical analysis revealed that TRPV1 was expressed in TG, and its expression was increased after experimental tooth movement. Western blot results also showed that experimental tooth movement led to a statistically significant increase in expression of TRPV1 protein in TG. Meanwhile, the time spent on directed face-grooming peaked on day 1 and thereafter showed a gradual decrease. In addition, both the change in TRPV1 expression in the TG and directed face-grooming behavior were modulated in a force-dependent manner and in concert with initial orthodontic pain responses. Our results reveal that TRPV1 expression is modulated by experimental tooth movement and is involved in tooth-movement pain. SN - 1600-0722 UR - https://www.unboundmedicine.com/medline/citation/25407056/Increased_expression_of_TRPV1_in_the_trigeminal_ganglion_is_involved_in_orofacial_pain_during_experimental_tooth_movement_in_rats_ L2 - https://doi.org/10.1111/eos.12158 DB - PRIME DP - Unbound Medicine ER -