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Schiff base formation with amino acids enhances light emission and damage induced in neutrophils by phenylacetaldehyde.
Biochim Biophys Acta 1989; 991(1):50-5BB

Abstract

The light emission and the loss of cell viability observed when phenylacetaldehyde is added to neutrophils are greatly enhanced when phenylacetaldehyde is administered as a Schiff base with amino acids. As in the case of phenylacetaldehyde, the Schiff base undergoes an intracellular, myeloperoxidase-catalyzed, oxygen-consuming process. Sonication of the cells enhances the emission. With both the free aldehyde and the Schiff bases, the emission spectrum peaks in the 490 nm region, whereas optically excited neutrophils and spent reaction mixtures show maximal emission elsewhere. Apparently, the primarily formed excited species (triplet benzaldehyde) either specifically transfers excitation energy to a component that makes only a minor contribution to the luminescence spectrum of the cells or initiates a process which is itself emissive, e.g. lipid peroxidation. As in the case of phenylacetaldehyde, the oxidation of the Schiff bases excites chlorophyll taken up by neutrophils. Loss of cell viability is likely to be related to in situ generation of excited species.

Authors+Show Affiliations

Departamento de Bioquímica, Universidade de São Paulo, Brazil.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2540843

Citation

Nascimento, A L., and G Cilento. "Schiff Base Formation With Amino Acids Enhances Light Emission and Damage Induced in Neutrophils By Phenylacetaldehyde." Biochimica Et Biophysica Acta, vol. 991, no. 1, 1989, pp. 50-5.
Nascimento AL, Cilento G. Schiff base formation with amino acids enhances light emission and damage induced in neutrophils by phenylacetaldehyde. Biochim Biophys Acta. 1989;991(1):50-5.
Nascimento, A. L., & Cilento, G. (1989). Schiff base formation with amino acids enhances light emission and damage induced in neutrophils by phenylacetaldehyde. Biochimica Et Biophysica Acta, 991(1), pp. 50-5.
Nascimento AL, Cilento G. Schiff Base Formation With Amino Acids Enhances Light Emission and Damage Induced in Neutrophils By Phenylacetaldehyde. Biochim Biophys Acta. 1989 Apr 25;991(1):50-5. PubMed PMID: 2540843.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Schiff base formation with amino acids enhances light emission and damage induced in neutrophils by phenylacetaldehyde. AU - Nascimento,A L, AU - Cilento,G, PY - 1989/4/25/pubmed PY - 1989/4/25/medline PY - 1989/4/25/entrez SP - 50 EP - 5 JF - Biochimica et biophysica acta JO - Biochim. Biophys. Acta VL - 991 IS - 1 N2 - The light emission and the loss of cell viability observed when phenylacetaldehyde is added to neutrophils are greatly enhanced when phenylacetaldehyde is administered as a Schiff base with amino acids. As in the case of phenylacetaldehyde, the Schiff base undergoes an intracellular, myeloperoxidase-catalyzed, oxygen-consuming process. Sonication of the cells enhances the emission. With both the free aldehyde and the Schiff bases, the emission spectrum peaks in the 490 nm region, whereas optically excited neutrophils and spent reaction mixtures show maximal emission elsewhere. Apparently, the primarily formed excited species (triplet benzaldehyde) either specifically transfers excitation energy to a component that makes only a minor contribution to the luminescence spectrum of the cells or initiates a process which is itself emissive, e.g. lipid peroxidation. As in the case of phenylacetaldehyde, the oxidation of the Schiff bases excites chlorophyll taken up by neutrophils. Loss of cell viability is likely to be related to in situ generation of excited species. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/2540843/Schiff_base_formation_with_amino_acids_enhances_light_emission_and_damage_induced_in_neutrophils_by_phenylacetaldehyde_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0304-4165(89)90027-5 DB - PRIME DP - Unbound Medicine ER -