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HPLC/QTOF-MS/MS application to investigate phenolic constituents from Ficus pandurata H. aerial roots.
Biomed Chromatogr. 2015 Jun; 29(6):860-8.BC

Abstract

Ficus pandurata H. aerial roots are used as a traditional Chinese medicine for the treatment of uarthritis, indigestion and hyperuricemia. However, the bioactive constituents responsible for the pharmacological effects of F. pandurata H. are unclear. A simple and efficient HPLC/QTOF-MS/MS (high-performance liquid chromatography/electrospray ionization with quadrupole time-of-flight tandem mass spectrometry) method was established to detect and identify active constituents in the n-butanol extract of F. pandurata H. aerial roots. Chemical constituents were separated and investigated by HPLC/QTOF-MS/MS in the negative-ion mode. Thirty-seven compounds, including hydroxycinnamic acid derivatives, hydroxybenzoic acid derivatives, hydroquinone glycosides, flavonoid glycosides, etc., were identified or tentatively characterized in the n-butanol extract of F. pandurata H. aerial roots by comparing the UV spectra, accurate mass spectra and fragmentation pathways and retrieving the reference literatures. Moreover, the flavonoid trisaccharides and hydroxybenzoic acid derivatives were tentatively characterized in F. pandurata H. for the first time. The analytical tool used here is very valuable in the rapid separation and identification of the multiple and minor constituents in the n-butanol extract of F. pandurata H. aerial roots.

Authors+Show Affiliations

College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, 310014, China. Key Laboratory of Organosilicon Chemistry and Material Technology, Hangzhou Normal University, Hangzhou, 310012, China.College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, China.College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, 310014, China.Key Laboratory of Organosilicon Chemistry and Material Technology, Hangzhou Normal University, Hangzhou, 310012, China.Department of Chemistry, National Chung-Hsing University, Taichung, 40227, Taiwan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25408477

Citation

Zhang, Xiaoping, et al. "HPLC/QTOF-MS/MS Application to Investigate Phenolic Constituents From Ficus Pandurata H. Aerial Roots." Biomedical Chromatography : BMC, vol. 29, no. 6, 2015, pp. 860-8.
Zhang X, Lv H, Li Z, et al. HPLC/QTOF-MS/MS application to investigate phenolic constituents from Ficus pandurata H. aerial roots. Biomed Chromatogr. 2015;29(6):860-8.
Zhang, X., Lv, H., Li, Z., Jiang, K., & Lee, M. R. (2015). HPLC/QTOF-MS/MS application to investigate phenolic constituents from Ficus pandurata H. aerial roots. Biomedical Chromatography : BMC, 29(6), 860-8. https://doi.org/10.1002/bmc.3366
Zhang X, et al. HPLC/QTOF-MS/MS Application to Investigate Phenolic Constituents From Ficus Pandurata H. Aerial Roots. Biomed Chromatogr. 2015;29(6):860-8. PubMed PMID: 25408477.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC/QTOF-MS/MS application to investigate phenolic constituents from Ficus pandurata H. aerial roots. AU - Zhang,Xiaoping, AU - Lv,Huiqing, AU - Li,Zuguang, AU - Jiang,Kezhi, AU - Lee,Maw-Rong, Y1 - 2014/11/18/ PY - 2014/03/11/received PY - 2014/09/11/revised PY - 2014/09/25/accepted PY - 2014/11/20/entrez PY - 2014/11/20/pubmed PY - 2016/2/11/medline KW - F. pandurata H KW - HPLC/QTOF-MS/MS KW - aerial roots KW - fragmentation pathways KW - phenolic constituents SP - 860 EP - 8 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 29 IS - 6 N2 - Ficus pandurata H. aerial roots are used as a traditional Chinese medicine for the treatment of uarthritis, indigestion and hyperuricemia. However, the bioactive constituents responsible for the pharmacological effects of F. pandurata H. are unclear. A simple and efficient HPLC/QTOF-MS/MS (high-performance liquid chromatography/electrospray ionization with quadrupole time-of-flight tandem mass spectrometry) method was established to detect and identify active constituents in the n-butanol extract of F. pandurata H. aerial roots. Chemical constituents were separated and investigated by HPLC/QTOF-MS/MS in the negative-ion mode. Thirty-seven compounds, including hydroxycinnamic acid derivatives, hydroxybenzoic acid derivatives, hydroquinone glycosides, flavonoid glycosides, etc., were identified or tentatively characterized in the n-butanol extract of F. pandurata H. aerial roots by comparing the UV spectra, accurate mass spectra and fragmentation pathways and retrieving the reference literatures. Moreover, the flavonoid trisaccharides and hydroxybenzoic acid derivatives were tentatively characterized in F. pandurata H. for the first time. The analytical tool used here is very valuable in the rapid separation and identification of the multiple and minor constituents in the n-butanol extract of F. pandurata H. aerial roots. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/25408477/HPLC/QTOF_MS/MS_application_to_investigate_phenolic_constituents_from_Ficus_pandurata_H__aerial_roots_ L2 - https://doi.org/10.1002/bmc.3366 DB - PRIME DP - Unbound Medicine ER -