Tags

Type your tag names separated by a space and hit enter

Dual effects of quercetin in doxorubicin-induced nephrotoxicity in rats and its modulation of the cytotoxic activity of doxorubicin on human carcinoma cells.
Environ Toxicol. 2016 May; 31(5):624-36.ET

Abstract

Quercetin (QUR) has been shown to induce anti-, as well as, pro-oxidant effects depending on the dose and on the redox state of the cell. This study investigated the effects of different doses of QUR on doxorubicin (DOX)-induced nephrotoxicity in rats with emphasis on the suggested mechanisms and its modulation of the cytotoxic activity of DOX on different human carcinoma cell lines. Three doses of QUR (10, 50, and 100 mg kg(-1)) were administered orally to adult male albino rats for 14 days, in the presence or absence of nephrotoxicity induced by a single intraperitoneal injection of DOX (15 mg kg(-1)) at day 7 of the experiment. Moreover, the effect of QUR in the presence of DOX on the cell viability of four different human cancer cell lines; PC3, HELA, MCF7, and HEPG2 was studied. Results showed that the lowest dose of QUR was more effective in preserving renal function (kidney index, blood urea nitrogen, serum creatinine, renal malondialdehyde, nitric oxide, reduced glutathione, catalase activity, renal expressions of TNF-α, IL-1B, iNOS, and caspase-3, and renal histopathology) than higher doses. Alternatively, the pro-oxidant and pro-inflammatory mechanisms have been reflected at highest QUR dose. In conclusion, QUR protected against DOX-induced nephrotoxicity with a provision to dosage adjustment. Furthermore, QUR did not interfere but rather enhanced the cytotoxic effects of DOX on different human cancer cell lines.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, El-Minia, Egypt.Department of Agricultural Chemistry, Faculty of Agriculture, Minia University, El-Minia, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25411067

Citation

Heeba, Gehan H., and Magda E. Mahmoud. "Dual Effects of Quercetin in Doxorubicin-induced Nephrotoxicity in Rats and Its Modulation of the Cytotoxic Activity of Doxorubicin On Human Carcinoma Cells." Environmental Toxicology, vol. 31, no. 5, 2016, pp. 624-36.
Heeba GH, Mahmoud ME. Dual effects of quercetin in doxorubicin-induced nephrotoxicity in rats and its modulation of the cytotoxic activity of doxorubicin on human carcinoma cells. Environ Toxicol. 2016;31(5):624-36.
Heeba, G. H., & Mahmoud, M. E. (2016). Dual effects of quercetin in doxorubicin-induced nephrotoxicity in rats and its modulation of the cytotoxic activity of doxorubicin on human carcinoma cells. Environmental Toxicology, 31(5), 624-36. https://doi.org/10.1002/tox.22075
Heeba GH, Mahmoud ME. Dual Effects of Quercetin in Doxorubicin-induced Nephrotoxicity in Rats and Its Modulation of the Cytotoxic Activity of Doxorubicin On Human Carcinoma Cells. Environ Toxicol. 2016;31(5):624-36. PubMed PMID: 25411067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dual effects of quercetin in doxorubicin-induced nephrotoxicity in rats and its modulation of the cytotoxic activity of doxorubicin on human carcinoma cells. AU - Heeba,Gehan H, AU - Mahmoud,Magda E, Y1 - 2014/11/20/ PY - 2014/07/14/received PY - 2014/10/31/accepted PY - 2014/11/21/entrez PY - 2014/11/21/pubmed PY - 2016/12/15/medline KW - apoptosis KW - cytotoxicity KW - doxorubicin KW - nephrotoxicity KW - oxidative stress KW - quercetin SP - 624 EP - 36 JF - Environmental toxicology JO - Environ Toxicol VL - 31 IS - 5 N2 - Quercetin (QUR) has been shown to induce anti-, as well as, pro-oxidant effects depending on the dose and on the redox state of the cell. This study investigated the effects of different doses of QUR on doxorubicin (DOX)-induced nephrotoxicity in rats with emphasis on the suggested mechanisms and its modulation of the cytotoxic activity of DOX on different human carcinoma cell lines. Three doses of QUR (10, 50, and 100 mg kg(-1)) were administered orally to adult male albino rats for 14 days, in the presence or absence of nephrotoxicity induced by a single intraperitoneal injection of DOX (15 mg kg(-1)) at day 7 of the experiment. Moreover, the effect of QUR in the presence of DOX on the cell viability of four different human cancer cell lines; PC3, HELA, MCF7, and HEPG2 was studied. Results showed that the lowest dose of QUR was more effective in preserving renal function (kidney index, blood urea nitrogen, serum creatinine, renal malondialdehyde, nitric oxide, reduced glutathione, catalase activity, renal expressions of TNF-α, IL-1B, iNOS, and caspase-3, and renal histopathology) than higher doses. Alternatively, the pro-oxidant and pro-inflammatory mechanisms have been reflected at highest QUR dose. In conclusion, QUR protected against DOX-induced nephrotoxicity with a provision to dosage adjustment. Furthermore, QUR did not interfere but rather enhanced the cytotoxic effects of DOX on different human cancer cell lines. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/25411067/Dual_effects_of_quercetin_in_doxorubicin_induced_nephrotoxicity_in_rats_and_its_modulation_of_the_cytotoxic_activity_of_doxorubicin_on_human_carcinoma_cells_ L2 - https://doi.org/10.1002/tox.22075 DB - PRIME DP - Unbound Medicine ER -