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CCl4 induced genotoxicity and DNA oxidative damages in rats: hepatoprotective effect of Sonchus arvensis.
BMC Complement Altern Med. 2014 Nov 21; 14:452.BC

Abstract

BACKGROUND

Sonchus arvesis is traditionally reported in various human ailments including hepatotoxicity in Pakistan. Presently we designed to assess the protective effects of methanolic extract of Sonchus arvesis against carbon tetrachloride induced genotoxicity and DNA oxidative damages in hepatic tissues of experimental rats.

METHODS

36 male Sprague-Dawley rats were randomly divided into 6 groups to evaluate the hepatoprotective effects of Sonchus arvensis against CCl4 induced genotoxicity, DNA damages and antioxidant depletion. Rats of normal control group were given free access of food and water add labitum. Group II rats received 3 ml/kg of CCl4 (30% in olive oil v/v) via the intraperitoneal route twice a week for four weeks. Group III and IV received 1 ml of 100 mg/kg b.w. and 200 mg/kg b.w. SME via gavage after 48 h of CCl4 treatment whereas group V was given 1 ml of silymarin (100 mg/kg b.w.) after 48 h of CCl4 treatment. Group VI only received 200 mg/kg b.w. SME. Protective effects of SME were checked by measuring serum markers, activities of antioxidant enzymes, genotoxicity and DNA dmages.

RESULTS

Results of the present study showed that treatment of SME reversed the activities of serum marker enzymes and cholesterol profile as depleted with CCl4 treatment. Activities of endogenous antioxidant enzymes of liver tissue homogenate; catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSHpx), glutathione-S-transferase (GST) and glutathione reductase (GSR) were reduced with administration of CCl4, which were returned to the control level with SME treatment. CCl4-induced hepatic cirrhosis decreased hepatic glutathione (GSH) and increased lipid peroxidative products (TBARS), were normalized by treatment with SME. Moreover, administration of CCl4 caused genotoxicity and DNA fragmentation which were significantly restored towards the normal level with SME.

CONCLUSION

These results reveal that treatment of SME may be useful in the prevention of hepatic stress.

Authors+Show Affiliations

No affiliation info availablePharmacology Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudia Arabia. Rahmatgul_81@yahoo.com.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25412679

Citation

Alkreathy, Huda Mohammad, et al. "CCl4 Induced Genotoxicity and DNA Oxidative Damages in Rats: Hepatoprotective Effect of Sonchus Arvensis." BMC Complementary and Alternative Medicine, vol. 14, 2014, p. 452.
Alkreathy HM, Khan RA, Khan MR, et al. CCl4 induced genotoxicity and DNA oxidative damages in rats: hepatoprotective effect of Sonchus arvensis. BMC Complement Altern Med. 2014;14:452.
Alkreathy, H. M., Khan, R. A., Khan, M. R., & Sahreen, S. (2014). CCl4 induced genotoxicity and DNA oxidative damages in rats: hepatoprotective effect of Sonchus arvensis. BMC Complementary and Alternative Medicine, 14, 452. https://doi.org/10.1186/1472-6882-14-452
Alkreathy HM, et al. CCl4 Induced Genotoxicity and DNA Oxidative Damages in Rats: Hepatoprotective Effect of Sonchus Arvensis. BMC Complement Altern Med. 2014 Nov 21;14:452. PubMed PMID: 25412679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CCl4 induced genotoxicity and DNA oxidative damages in rats: hepatoprotective effect of Sonchus arvensis. AU - Alkreathy,Huda Mohammad, AU - Khan,Rahmat Ali, AU - Khan,Muhammad Rashid, AU - Sahreen,Sumaira, Y1 - 2014/11/21/ PY - 2014/01/17/received PY - 2014/10/06/accepted PY - 2014/11/22/entrez PY - 2014/11/22/pubmed PY - 2015/6/9/medline SP - 452 EP - 452 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 14 N2 - BACKGROUND: Sonchus arvesis is traditionally reported in various human ailments including hepatotoxicity in Pakistan. Presently we designed to assess the protective effects of methanolic extract of Sonchus arvesis against carbon tetrachloride induced genotoxicity and DNA oxidative damages in hepatic tissues of experimental rats. METHODS: 36 male Sprague-Dawley rats were randomly divided into 6 groups to evaluate the hepatoprotective effects of Sonchus arvensis against CCl4 induced genotoxicity, DNA damages and antioxidant depletion. Rats of normal control group were given free access of food and water add labitum. Group II rats received 3 ml/kg of CCl4 (30% in olive oil v/v) via the intraperitoneal route twice a week for four weeks. Group III and IV received 1 ml of 100 mg/kg b.w. and 200 mg/kg b.w. SME via gavage after 48 h of CCl4 treatment whereas group V was given 1 ml of silymarin (100 mg/kg b.w.) after 48 h of CCl4 treatment. Group VI only received 200 mg/kg b.w. SME. Protective effects of SME were checked by measuring serum markers, activities of antioxidant enzymes, genotoxicity and DNA dmages. RESULTS: Results of the present study showed that treatment of SME reversed the activities of serum marker enzymes and cholesterol profile as depleted with CCl4 treatment. Activities of endogenous antioxidant enzymes of liver tissue homogenate; catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSHpx), glutathione-S-transferase (GST) and glutathione reductase (GSR) were reduced with administration of CCl4, which were returned to the control level with SME treatment. CCl4-induced hepatic cirrhosis decreased hepatic glutathione (GSH) and increased lipid peroxidative products (TBARS), were normalized by treatment with SME. Moreover, administration of CCl4 caused genotoxicity and DNA fragmentation which were significantly restored towards the normal level with SME. CONCLUSION: These results reveal that treatment of SME may be useful in the prevention of hepatic stress. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/25412679/CCl4_induced_genotoxicity_and_DNA_oxidative_damages_in_rats:_hepatoprotective_effect_of_Sonchus_arvensis_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-452 DB - PRIME DP - Unbound Medicine ER -