Tags

Type your tag names separated by a space and hit enter

A synergistic effect of simultaneous TRPA1 and TRPV1 activations on vagal pulmonary C-fiber afferents.
J Appl Physiol (1985). 2015 Feb 01; 118(3):273-81.JA

Abstract

Transient receptor potential ankyrin type 1 (TRPA1) and vanilloid type 1 (TRPV1) receptors are coexpressed in vagal pulmonary C-fiber sensory nerves. Because both these receptors are sensitive to a number of endogenous inflammatory mediators, it is conceivable that they can be activated simultaneously during airway inflammation. This study aimed to determine whether there is an interaction between these two polymodal transducers upon simultaneous activation, and how it modulates the activity of vagal pulmonary C-fiber sensory nerves. In anesthetized, spontaneously breathing rats, the reflex-mediated apneic response to intravenous injection of a combined dose of allyl isothiocyanate (AITC, a TRPA1 activator) and capsaicin (Cap, a TRPV1 activator) was ∼202% greater than the mathematical sum of the responses to AITC and Cap when they were administered individually. Similar results were also observed in anesthetized mice. In addition, the synergistic effect was clearly demonstrated when the afferent activity of single vagal pulmonary C-fiber afferents were recorded in anesthetized, artificially ventilated rats; C-fiber responses to AITC, Cap and AITC + Cap (in combination) were 0.6 ± 0.1, 0.8 ± 0.1, and 4.8 ± 0.6 impulses/s (n = 24), respectively. This synergism was absent when either AITC or Cap was replaced by other chemical activators of pulmonary C-fiber afferents. The pronounced potentiating effect was further demonstrated in isolated vagal pulmonary sensory neurons using the Ca(2+) imaging technique. In summary, this study showed a distinct positive interaction between TRPA1 and TRPV1 when they were activated simultaneously in pulmonary C-fiber sensory nerves.

Authors+Show Affiliations

Department of Physiology, University of Kentucky Medical Center, Lexington, Kentucky;Department of Physiology, University of Kentucky Medical Center, Lexington, Kentucky;School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan; and.Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of Kentucky Medical Center, Lexington, Kentucky.Department of Physiology, University of Kentucky Medical Center, Lexington, Kentucky; lylee@uky.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

25414245

Citation

Lin, Yu-Jung, et al. "A Synergistic Effect of Simultaneous TRPA1 and TRPV1 Activations On Vagal Pulmonary C-fiber Afferents." Journal of Applied Physiology (Bethesda, Md. : 1985), vol. 118, no. 3, 2015, pp. 273-81.
Lin YJ, Lin RL, Ruan T, et al. A synergistic effect of simultaneous TRPA1 and TRPV1 activations on vagal pulmonary C-fiber afferents. J Appl Physiol (1985). 2015;118(3):273-81.
Lin, Y. J., Lin, R. L., Ruan, T., Khosravi, M., & Lee, L. Y. (2015). A synergistic effect of simultaneous TRPA1 and TRPV1 activations on vagal pulmonary C-fiber afferents. Journal of Applied Physiology (Bethesda, Md. : 1985), 118(3), 273-81. https://doi.org/10.1152/japplphysiol.00805.2014
Lin YJ, et al. A Synergistic Effect of Simultaneous TRPA1 and TRPV1 Activations On Vagal Pulmonary C-fiber Afferents. J Appl Physiol (1985). 2015 Feb 1;118(3):273-81. PubMed PMID: 25414245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A synergistic effect of simultaneous TRPA1 and TRPV1 activations on vagal pulmonary C-fiber afferents. AU - Lin,Yu-Jung, AU - Lin,Ruei-Lung, AU - Ruan,Ting, AU - Khosravi,Mehdi, AU - Lee,Lu-Yuan, Y1 - 2014/11/20/ PY - 2014/11/22/entrez PY - 2014/11/22/pubmed PY - 2015/9/29/medline KW - AITC KW - TRPA1 KW - TRPV1 KW - capsaicin KW - inflammation SP - 273 EP - 81 JF - Journal of applied physiology (Bethesda, Md. : 1985) JO - J Appl Physiol (1985) VL - 118 IS - 3 N2 - Transient receptor potential ankyrin type 1 (TRPA1) and vanilloid type 1 (TRPV1) receptors are coexpressed in vagal pulmonary C-fiber sensory nerves. Because both these receptors are sensitive to a number of endogenous inflammatory mediators, it is conceivable that they can be activated simultaneously during airway inflammation. This study aimed to determine whether there is an interaction between these two polymodal transducers upon simultaneous activation, and how it modulates the activity of vagal pulmonary C-fiber sensory nerves. In anesthetized, spontaneously breathing rats, the reflex-mediated apneic response to intravenous injection of a combined dose of allyl isothiocyanate (AITC, a TRPA1 activator) and capsaicin (Cap, a TRPV1 activator) was ∼202% greater than the mathematical sum of the responses to AITC and Cap when they were administered individually. Similar results were also observed in anesthetized mice. In addition, the synergistic effect was clearly demonstrated when the afferent activity of single vagal pulmonary C-fiber afferents were recorded in anesthetized, artificially ventilated rats; C-fiber responses to AITC, Cap and AITC + Cap (in combination) were 0.6 ± 0.1, 0.8 ± 0.1, and 4.8 ± 0.6 impulses/s (n = 24), respectively. This synergism was absent when either AITC or Cap was replaced by other chemical activators of pulmonary C-fiber afferents. The pronounced potentiating effect was further demonstrated in isolated vagal pulmonary sensory neurons using the Ca(2+) imaging technique. In summary, this study showed a distinct positive interaction between TRPA1 and TRPV1 when they were activated simultaneously in pulmonary C-fiber sensory nerves. SN - 1522-1601 UR - https://www.unboundmedicine.com/medline/citation/25414245/A_synergistic_effect_of_simultaneous_TRPA1_and_TRPV1_activations_on_vagal_pulmonary_C_fiber_afferents_ L2 - https://journals.physiology.org/doi/10.1152/japplphysiol.00805.2014?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -