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Genome-wide analysis of the Hsf family in soybean and functional identification of GmHsf-34 involvement in drought and heat stresses.
BMC Genomics. 2014 Nov 21; 15:1009.BG

Abstract

BACKGROUND

High temperature affects organism growth and metabolic activity. Heat shock transcription factors (Hsfs) are key regulators in heat shock response in eukaryotes and prokaryotes. Under high temperature conditions, Hsfs activate heat shock proteins (Hsps) by combining with heat stress elements (HSEs) in their promoters, leading to defense of heat stress. Since the first plant Hsf gene was identified in tomato, several plant Hsf family genes have been thoroughly characterized. Although soybean (Glycine max), an important oilseed crops, genome sequences have been available, the Hsf family genes in soybean have not been characterized accurately.

RESULT

We analyzed the Hsf genetic structures and protein function domains using the GSDS, Pfam, SMART, PredictNLS, and NetNES online tools. The genome scanning of dicots (soybean and Arabidopsis) and monocots (rice and maize) revealed that the whole-genome replication occurred twice in soybean evolution. The plant Hsfs were classified into 3 classes and 16 subclasses according to protein structure domains. The A8 and B3 subclasses existed only in dicots and the A9 and C2 occurred only in monocots. Thirty eight soybean Hsfs were systematically identified and grouped into 3 classes and 12 subclasses, and located on 15 soybean chromosomes. The promoter regions of the soybean Hsfs contained cis-elements that likely participate in drought, low temperature, and ABA stress responses. There were large differences among Hsfs based on transcriptional levels under the stress conditions. The transcriptional levels of the A1 and A2 subclass genes were extraordinarily high. In addition, differences in the expression levels occurred for each gene in the different organs and at the different developmental stages. Several genes were chosen to determine their subcellular localizations and functions. The subcellular localization results revealed that GmHsf-04, GmHsf-33, and GmHsf-34 were located in the nucleus. Overexpression of the GmHsf-34 gene improved the tolerances to drought and heat stresses in Arabidopsis plants.

CONCLUSIONS

This present investigation of the quantity, structural features, expression characteristics, subcellular localizations, and functional roles provides a scientific basis for further research on soybean Hsf functions.

Authors+Show Affiliations

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableCollege of Agronomy, Northwest A & F University, Yangling 712100, China. chaishoucheng@126.com.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25416131

Citation

Li, Pan-Song, et al. "Genome-wide Analysis of the Hsf Family in Soybean and Functional Identification of GmHsf-34 Involvement in Drought and Heat Stresses." BMC Genomics, vol. 15, 2014, p. 1009.
Li PS, Yu TF, He GH, et al. Genome-wide analysis of the Hsf family in soybean and functional identification of GmHsf-34 involvement in drought and heat stresses. BMC Genomics. 2014;15:1009.
Li, P. S., Yu, T. F., He, G. H., Chen, M., Zhou, Y. B., Chai, S. C., Xu, Z. S., & Ma, Y. Z. (2014). Genome-wide analysis of the Hsf family in soybean and functional identification of GmHsf-34 involvement in drought and heat stresses. BMC Genomics, 15, 1009. https://doi.org/10.1186/1471-2164-15-1009
Li PS, et al. Genome-wide Analysis of the Hsf Family in Soybean and Functional Identification of GmHsf-34 Involvement in Drought and Heat Stresses. BMC Genomics. 2014 Nov 21;15:1009. PubMed PMID: 25416131.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide analysis of the Hsf family in soybean and functional identification of GmHsf-34 involvement in drought and heat stresses. AU - Li,Pan-Song, AU - Yu,Tai-Fei, AU - He,Guan-Hua, AU - Chen,Ming, AU - Zhou,Yong-Bin, AU - Chai,Shou-Cheng, AU - Xu,Zhao-Shi, AU - Ma,You-Zhi, Y1 - 2014/11/21/ PY - 2014/08/29/received PY - 2014/10/08/accepted PY - 2014/11/23/entrez PY - 2014/11/25/pubmed PY - 2015/7/7/medline SP - 1009 EP - 1009 JF - BMC genomics JO - BMC Genomics VL - 15 N2 - BACKGROUND: High temperature affects organism growth and metabolic activity. Heat shock transcription factors (Hsfs) are key regulators in heat shock response in eukaryotes and prokaryotes. Under high temperature conditions, Hsfs activate heat shock proteins (Hsps) by combining with heat stress elements (HSEs) in their promoters, leading to defense of heat stress. Since the first plant Hsf gene was identified in tomato, several plant Hsf family genes have been thoroughly characterized. Although soybean (Glycine max), an important oilseed crops, genome sequences have been available, the Hsf family genes in soybean have not been characterized accurately. RESULT: We analyzed the Hsf genetic structures and protein function domains using the GSDS, Pfam, SMART, PredictNLS, and NetNES online tools. The genome scanning of dicots (soybean and Arabidopsis) and monocots (rice and maize) revealed that the whole-genome replication occurred twice in soybean evolution. The plant Hsfs were classified into 3 classes and 16 subclasses according to protein structure domains. The A8 and B3 subclasses existed only in dicots and the A9 and C2 occurred only in monocots. Thirty eight soybean Hsfs were systematically identified and grouped into 3 classes and 12 subclasses, and located on 15 soybean chromosomes. The promoter regions of the soybean Hsfs contained cis-elements that likely participate in drought, low temperature, and ABA stress responses. There were large differences among Hsfs based on transcriptional levels under the stress conditions. The transcriptional levels of the A1 and A2 subclass genes were extraordinarily high. In addition, differences in the expression levels occurred for each gene in the different organs and at the different developmental stages. Several genes were chosen to determine their subcellular localizations and functions. The subcellular localization results revealed that GmHsf-04, GmHsf-33, and GmHsf-34 were located in the nucleus. Overexpression of the GmHsf-34 gene improved the tolerances to drought and heat stresses in Arabidopsis plants. CONCLUSIONS: This present investigation of the quantity, structural features, expression characteristics, subcellular localizations, and functional roles provides a scientific basis for further research on soybean Hsf functions. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/25416131/Genome_wide_analysis_of_the_Hsf_family_in_soybean_and_functional_identification_of_GmHsf_34_involvement_in_drought_and_heat_stresses_ L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-1009 DB - PRIME DP - Unbound Medicine ER -