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Replication and shedding of MERS-CoV in upper respiratory tract of inoculated dromedary camels.
Emerg Infect Dis. 2014 Dec; 20(12):1999-2005.EI

Abstract

In 2012, a novel coronavirus associated with severe respiratory disease in humans emerged in the Middle East. Epidemiologic investigations identified dromedary camels as the likely source of zoonotic transmission of Middle East respiratory syndrome coronavirus (MERS-CoV). Here we provide experimental support for camels as a reservoir for MERS-CoV. We inoculated 3 adult camels with a human isolate of MERS-CoV and a transient, primarily upper respiratory tract infection developed in each of the 3 animals. Clinical signs of the MERS-CoV infection were benign, but each of the camels shed large quantities of virus from the upper respiratory tract. We detected infectious virus in nasal secretions through 7 days postinoculation, and viral RNA up to 35 days postinoculation. The pattern of shedding and propensity for the upper respiratory tract infection in dromedary camels may help explain the lack of systemic illness among naturally infected camels and the means of efficient camel-to-camel and camel-to-human transmission.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

25418529

Citation

Adney, Danielle R., et al. "Replication and Shedding of MERS-CoV in Upper Respiratory Tract of Inoculated Dromedary Camels." Emerging Infectious Diseases, vol. 20, no. 12, 2014, pp. 1999-2005.
Adney DR, van Doremalen N, Brown VR, et al. Replication and shedding of MERS-CoV in upper respiratory tract of inoculated dromedary camels. Emerg Infect Dis. 2014;20(12):1999-2005.
Adney, D. R., van Doremalen, N., Brown, V. R., Bushmaker, T., Scott, D., de Wit, E., Bowen, R. A., & Munster, V. J. (2014). Replication and shedding of MERS-CoV in upper respiratory tract of inoculated dromedary camels. Emerging Infectious Diseases, 20(12), 1999-2005. https://doi.org/10.3201/eid2012.141280
Adney DR, et al. Replication and Shedding of MERS-CoV in Upper Respiratory Tract of Inoculated Dromedary Camels. Emerg Infect Dis. 2014;20(12):1999-2005. PubMed PMID: 25418529.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Replication and shedding of MERS-CoV in upper respiratory tract of inoculated dromedary camels. AU - Adney,Danielle R, AU - van Doremalen,Neeltje, AU - Brown,Vienna R, AU - Bushmaker,Trenton, AU - Scott,Dana, AU - de Wit,Emmie, AU - Bowen,Richard A, AU - Munster,Vincent J, PY - 2014/11/25/entrez PY - 2014/11/25/pubmed PY - 2016/5/14/medline SP - 1999 EP - 2005 JF - Emerging infectious diseases JO - Emerg Infect Dis VL - 20 IS - 12 N2 - In 2012, a novel coronavirus associated with severe respiratory disease in humans emerged in the Middle East. Epidemiologic investigations identified dromedary camels as the likely source of zoonotic transmission of Middle East respiratory syndrome coronavirus (MERS-CoV). Here we provide experimental support for camels as a reservoir for MERS-CoV. We inoculated 3 adult camels with a human isolate of MERS-CoV and a transient, primarily upper respiratory tract infection developed in each of the 3 animals. Clinical signs of the MERS-CoV infection were benign, but each of the camels shed large quantities of virus from the upper respiratory tract. We detected infectious virus in nasal secretions through 7 days postinoculation, and viral RNA up to 35 days postinoculation. The pattern of shedding and propensity for the upper respiratory tract infection in dromedary camels may help explain the lack of systemic illness among naturally infected camels and the means of efficient camel-to-camel and camel-to-human transmission. SN - 1080-6059 UR - https://www.unboundmedicine.com/medline/citation/25418529/Replication_and_shedding_of_MERS_CoV_in_upper_respiratory_tract_of_inoculated_dromedary_camels_ L2 - https://dx.doi.org/10.3201/eid2012.141280 DB - PRIME DP - Unbound Medicine ER -