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In vitro antibacterial phenolic extracts from "sugarbag" pot-honeys of Australian stingless bees (Tetragonula carbonaria).
J Agric Food Chem 2014; 62(50):12209-17JA

Abstract

Australian stingless bee honeys have been shown to exert antioxidant and in vitro antimicrobial properties; however their bioactive factors remained unidentified. This study investigated the antibacterial properties of phenolic extracts from Tetragonula carbonaria honeys. Honeys were harvested from beehives in three sites of South East Australia. Liquid-liquid extractions yielded the phenolic concentrates, for analyses by liquid and gas chromatography mass spectrometry. Antibacterial assays were conducted against Staphylococcus aureus and Klebsiella pneumoniae by in vitro agar diffusion and broth dilution assays. The phenolic extracts averaged to 5.87 mg/100 g of raw honeys, and constituents were 3-phenyllactic acid, lumichrome, diglycosylflavonoids, norisoprenoids. The honeys did not contain methylglyoxal, dihydroxyacetone or phenolics characteristic of Leptospermum nectars. Hydrogen peroxide content amounted up to 155.8 μM in honeys. Beside the bactericidal effects of hydrogen peroxide at 760 μM, other antibacterial factors were the phenolic extracts of "sugarbag" honeys that were active at minimum bactericidal concentrations of 1.2-1.8 mg/mL.

Authors+Show Affiliations

Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast , Maroochydore DC, Queensland, Australia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25423113

Citation

Massaro, C Flavia, et al. "In Vitro Antibacterial Phenolic Extracts From "sugarbag" Pot-honeys of Australian Stingless Bees (Tetragonula Carbonaria)." Journal of Agricultural and Food Chemistry, vol. 62, no. 50, 2014, pp. 12209-17.
Massaro CF, Shelley D, Heard TA, et al. In vitro antibacterial phenolic extracts from "sugarbag" pot-honeys of Australian stingless bees (Tetragonula carbonaria). J Agric Food Chem. 2014;62(50):12209-17.
Massaro, C. F., Shelley, D., Heard, T. A., & Brooks, P. (2014). In vitro antibacterial phenolic extracts from "sugarbag" pot-honeys of Australian stingless bees (Tetragonula carbonaria). Journal of Agricultural and Food Chemistry, 62(50), pp. 12209-17. doi:10.1021/jf5051848.
Massaro CF, et al. In Vitro Antibacterial Phenolic Extracts From "sugarbag" Pot-honeys of Australian Stingless Bees (Tetragonula Carbonaria). J Agric Food Chem. 2014 Dec 17;62(50):12209-17. PubMed PMID: 25423113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antibacterial phenolic extracts from "sugarbag" pot-honeys of Australian stingless bees (Tetragonula carbonaria). AU - Massaro,C Flavia, AU - Shelley,Daniel, AU - Heard,Tim A, AU - Brooks,Peter, Y1 - 2014/12/03/ PY - 2014/11/26/entrez PY - 2014/11/26/pubmed PY - 2015/8/19/medline KW - Meliponini KW - S. aureus KW - flavonoids KW - norisoprenoids KW - phloroglucinols KW - propolis SP - 12209 EP - 17 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 62 IS - 50 N2 - Australian stingless bee honeys have been shown to exert antioxidant and in vitro antimicrobial properties; however their bioactive factors remained unidentified. This study investigated the antibacterial properties of phenolic extracts from Tetragonula carbonaria honeys. Honeys were harvested from beehives in three sites of South East Australia. Liquid-liquid extractions yielded the phenolic concentrates, for analyses by liquid and gas chromatography mass spectrometry. Antibacterial assays were conducted against Staphylococcus aureus and Klebsiella pneumoniae by in vitro agar diffusion and broth dilution assays. The phenolic extracts averaged to 5.87 mg/100 g of raw honeys, and constituents were 3-phenyllactic acid, lumichrome, diglycosylflavonoids, norisoprenoids. The honeys did not contain methylglyoxal, dihydroxyacetone or phenolics characteristic of Leptospermum nectars. Hydrogen peroxide content amounted up to 155.8 μM in honeys. Beside the bactericidal effects of hydrogen peroxide at 760 μM, other antibacterial factors were the phenolic extracts of "sugarbag" honeys that were active at minimum bactericidal concentrations of 1.2-1.8 mg/mL. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/25423113/In_vitro_antibacterial_phenolic_extracts_from_"sugarbag"_pot_honeys_of_Australian_stingless_bees__Tetragonula_carbonaria__ L2 - https://dx.doi.org/10.1021/jf5051848 DB - PRIME DP - Unbound Medicine ER -