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β-asarone and levodopa co-administration protects against 6-hydroxydopamine-induced damage in parkinsonian rat mesencephalon by regulating autophagy: down-expression Beclin-1 and light chain 3B and up-expression P62.
Clin Exp Pharmacol Physiol 2015; 42(3):269-77CE

Abstract

In this study, we investigated Beclin-1, light chain (LC)3B, and p62 expression in 6-hydroxydopamine (6-OHDA)-induced parkinsonian rats after β-asarone and levodopa (l-dopa) co-administration. Unilateral 6-OHDA injection into the medial forebrain bundle was used to create the models, except in sham-operated rats. Rats were divided into eight groups: sham-operated group; 6-OHDA model group; madopar group (75 mg/kg, per os (p.o.)); l-dopa group (60 mg/kg, p.o.); β-asarone group (15 mg/kg, p.o.); β-asarone + l-dopa co-administered group (15 mg/kg + 60 mg/kg, p.o.); 3-methyladenine group (500 nmol, intraperitoneal injection); and rapamycin group (1 mg/kg, intraperitoneal injection). Then, Beclin-1, LC3B, and p62 expression in the mesencephalon were detected. The mesencephalon was also observed by transmission electron microscope. The results showed that Beclin-1 and LC3B expression decreased and that p62 expression increased significantly in the madopar, l-dopa, β-asarone, and co-administered groups when compared with the 6-OHDA model. Beclin-1 and LC3B expression in the β-asarone and co-administered groups were less than in the madopar or l-dopa groups, whereas p62 expression in the β-asarone and co-administered groups was higher than in the madopar or l-dopa groups. In addition, a significant decrease in autophagosome was exhibited in the β-asarone and co-administered groups when compared with the 6-OHDA group. Our findings indicate that Beclin-1 and LC3B expression decreased, whereas p62 expression increased after co-administration treatment. In sum, all data suggest that the co-administration of β-asarone and l-dopa may contribute to the treatment of 6-OHDA-induced damage in rats by inhibiting autophagy activity.

Authors+Show Affiliations

Experimental Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25424835

Citation

Huang, Li-Ping, et al. "Β-asarone and Levodopa Co-administration Protects Against 6-hydroxydopamine-induced Damage in Parkinsonian Rat Mesencephalon By Regulating Autophagy: Down-expression Beclin-1 and Light Chain 3B and Up-expression P62." Clinical and Experimental Pharmacology & Physiology, vol. 42, no. 3, 2015, pp. 269-77.
Huang LP, Deng MZ, He YP, et al. Β-asarone and levodopa co-administration protects against 6-hydroxydopamine-induced damage in parkinsonian rat mesencephalon by regulating autophagy: down-expression Beclin-1 and light chain 3B and up-expression P62. Clin Exp Pharmacol Physiol. 2015;42(3):269-77.
Huang, L. P., Deng, M. Z., He, Y. P., & Fang, Y. Q. (2015). Β-asarone and levodopa co-administration protects against 6-hydroxydopamine-induced damage in parkinsonian rat mesencephalon by regulating autophagy: down-expression Beclin-1 and light chain 3B and up-expression P62. Clinical and Experimental Pharmacology & Physiology, 42(3), pp. 269-77. doi:10.1111/1440-1681.12344.
Huang LP, et al. Β-asarone and Levodopa Co-administration Protects Against 6-hydroxydopamine-induced Damage in Parkinsonian Rat Mesencephalon By Regulating Autophagy: Down-expression Beclin-1 and Light Chain 3B and Up-expression P62. Clin Exp Pharmacol Physiol. 2015;42(3):269-77. PubMed PMID: 25424835.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - β-asarone and levodopa co-administration protects against 6-hydroxydopamine-induced damage in parkinsonian rat mesencephalon by regulating autophagy: down-expression Beclin-1 and light chain 3B and up-expression P62. AU - Huang,Li-Ping, AU - Deng,Min-Zhen, AU - He,Yu-Ping, AU - Fang,Yong-Qi, PY - 2014/09/29/received PY - 2014/11/07/revised PY - 2014/11/11/accepted PY - 2014/11/27/entrez PY - 2014/11/27/pubmed PY - 2015/10/28/medline KW - Beclin-1 KW - autophagy KW - levodopa KW - light chain 3B KW - p62 KW - β-asarone SP - 269 EP - 77 JF - Clinical and experimental pharmacology & physiology JO - Clin. Exp. Pharmacol. Physiol. VL - 42 IS - 3 N2 - In this study, we investigated Beclin-1, light chain (LC)3B, and p62 expression in 6-hydroxydopamine (6-OHDA)-induced parkinsonian rats after β-asarone and levodopa (l-dopa) co-administration. Unilateral 6-OHDA injection into the medial forebrain bundle was used to create the models, except in sham-operated rats. Rats were divided into eight groups: sham-operated group; 6-OHDA model group; madopar group (75 mg/kg, per os (p.o.)); l-dopa group (60 mg/kg, p.o.); β-asarone group (15 mg/kg, p.o.); β-asarone + l-dopa co-administered group (15 mg/kg + 60 mg/kg, p.o.); 3-methyladenine group (500 nmol, intraperitoneal injection); and rapamycin group (1 mg/kg, intraperitoneal injection). Then, Beclin-1, LC3B, and p62 expression in the mesencephalon were detected. The mesencephalon was also observed by transmission electron microscope. The results showed that Beclin-1 and LC3B expression decreased and that p62 expression increased significantly in the madopar, l-dopa, β-asarone, and co-administered groups when compared with the 6-OHDA model. Beclin-1 and LC3B expression in the β-asarone and co-administered groups were less than in the madopar or l-dopa groups, whereas p62 expression in the β-asarone and co-administered groups was higher than in the madopar or l-dopa groups. In addition, a significant decrease in autophagosome was exhibited in the β-asarone and co-administered groups when compared with the 6-OHDA group. Our findings indicate that Beclin-1 and LC3B expression decreased, whereas p62 expression increased after co-administration treatment. In sum, all data suggest that the co-administration of β-asarone and l-dopa may contribute to the treatment of 6-OHDA-induced damage in rats by inhibiting autophagy activity. SN - 1440-1681 UR - https://www.unboundmedicine.com/medline/citation/25424835/β_asarone_and_levodopa_co_administration_protects_against_6_hydroxydopamine_induced_damage_in_parkinsonian_rat_mesencephalon_by_regulating_autophagy:_down_expression_Beclin_1_and_light_chain_3B_and_up_expression_P62_ L2 - https://doi.org/10.1111/1440-1681.12344 DB - PRIME DP - Unbound Medicine ER -