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Electric stimulation at 448 kHz promotes proliferation of human mesenchymal stem cells.
Cell Physiol Biochem. 2014; 34(5):1741-55.CP

Abstract

BACKGROUND/AIMS

Capacitive-resistive electric transfer (CRET) is a non invasive electrothermal therapy that applies electric currents within the 400 kHz - 450 kHz frequency range to the treatment of musculoskeletal lesions. Evidence exists that electric currents and electric or magnetic fields can influence proliferative and/or differentiating processes involved in tissue regeneration. This work investigates proliferative responses potentially underlying CRET effects on tissue repair.

METHODS

XTT assay, flow cytometry, immunofluorescence and Western Blot analyses were conducted to asses viability, proliferation and differentiation of adipose-derived stem cells (ADSC) from healthy donors, after short, repeated (5 m On/4 h Off) in vitro stimulation with a 448-kHz electric signal currently used in CRET therapy, applied at a subthermal dose of 50 μA/mm(2) RESULTS: The treatment induced PCNA and ERK1/2 upregulation, together with significant increases in the fractions of ADSC undergoing cycle phases S, G2 and M, and enhanced cell proliferation rate. This proliferative effect did not compromise the multipotential ability of ADSC for subsequent adipogenic, chondrogenic or osteogenic differentiation.

CONCLUSIONS

These data identify cellular and molecular phenomena potentially underlying the response to CRET and indicate that CRET-induced lesion repair could be mediated by stimulation of the proliferation of stem cells present in the injured tissues.

Authors+Show Affiliations

Departamento de Investigación-BEM, Hospital Universitario Ramón y Cajal - IRYCIS, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25427571

Citation

Hernández-Bule, María Luisa, et al. "Electric Stimulation at 448 kHz Promotes Proliferation of Human Mesenchymal Stem Cells." Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, vol. 34, no. 5, 2014, pp. 1741-55.
Hernández-Bule ML, Paíno CL, Trillo MÁ, et al. Electric stimulation at 448 kHz promotes proliferation of human mesenchymal stem cells. Cell Physiol Biochem. 2014;34(5):1741-55.
Hernández-Bule, M. L., Paíno, C. L., Trillo, M. Á., & Úbeda, A. (2014). Electric stimulation at 448 kHz promotes proliferation of human mesenchymal stem cells. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 34(5), 1741-55. https://doi.org/10.1159/000366375
Hernández-Bule ML, et al. Electric Stimulation at 448 kHz Promotes Proliferation of Human Mesenchymal Stem Cells. Cell Physiol Biochem. 2014;34(5):1741-55. PubMed PMID: 25427571.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electric stimulation at 448 kHz promotes proliferation of human mesenchymal stem cells. AU - Hernández-Bule,María Luisa, AU - Paíno,Carlos Luis, AU - Trillo,María Ángeles, AU - Úbeda,Alejandro, Y1 - 2014/11/12/ PY - 2014/09/04/accepted PY - 2014/11/28/entrez PY - 2014/11/28/pubmed PY - 2015/8/15/medline SP - 1741 EP - 55 JF - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology JO - Cell. Physiol. Biochem. VL - 34 IS - 5 N2 - BACKGROUND/AIMS: Capacitive-resistive electric transfer (CRET) is a non invasive electrothermal therapy that applies electric currents within the 400 kHz - 450 kHz frequency range to the treatment of musculoskeletal lesions. Evidence exists that electric currents and electric or magnetic fields can influence proliferative and/or differentiating processes involved in tissue regeneration. This work investigates proliferative responses potentially underlying CRET effects on tissue repair. METHODS: XTT assay, flow cytometry, immunofluorescence and Western Blot analyses were conducted to asses viability, proliferation and differentiation of adipose-derived stem cells (ADSC) from healthy donors, after short, repeated (5 m On/4 h Off) in vitro stimulation with a 448-kHz electric signal currently used in CRET therapy, applied at a subthermal dose of 50 μA/mm(2) RESULTS: The treatment induced PCNA and ERK1/2 upregulation, together with significant increases in the fractions of ADSC undergoing cycle phases S, G2 and M, and enhanced cell proliferation rate. This proliferative effect did not compromise the multipotential ability of ADSC for subsequent adipogenic, chondrogenic or osteogenic differentiation. CONCLUSIONS: These data identify cellular and molecular phenomena potentially underlying the response to CRET and indicate that CRET-induced lesion repair could be mediated by stimulation of the proliferation of stem cells present in the injured tissues. SN - 1421-9778 UR - https://www.unboundmedicine.com/medline/citation/25427571/Electric_Stimulation_at_448_kHz_Promotes_Proliferation_of_Human_Mesenchymal_Stem_Cells_ L2 - https://www.karger.com?DOI=10.1159/000366375 DB - PRIME DP - Unbound Medicine ER -