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DOP-PCR based painting of rye chromosomes in a wheat background.
Genome. 2014 Sep; 57(9):473-9.G

Abstract

To determine the appropriateness of chromosome painting for identifying genomic elements in rye, we microdissected the 1R and 1RS chromosomes from rye (Secale cereale L. var. King II) and wheat-rye addition line 1RS, respectively. Degenerate oligonucleotide primed - polymerase chain reaction (DOP-PCR) amplification of 1R and 1RS products from dissected chromosomes were used as probes to hybridize to metaphase chromosomes of rye, wheat-rye addition lines 1R and 1RS, translocation line 1RS.1BL, and allohexaploid triticale. The results showed that (i) the hybridization signal distribution patterns on rye chromosomes using 1R-derived DOP-PCR products as the probe were similar to those using 1RS-derived DOP-PCR products as the probe; (ii) 1R and (or) 1RS could not be distinguished from other rye chromosomes solely by the hybridization patterns using 1R- and (or) 1RS-derived DOP-PCR products as the probe; (iii) rye chromosomes and (or) rye chromosome fragments could be clearly identified in wheat-rye hybrids using either 1R- or 1RS-derived DOP-PCR products as the probe and could be more accurate in the nontelomeric region than using genomic in situ hybridization (GISH). Our results suggested that 1R- and (or) 1RS-derived DOP-PCR products contain many repetitive DNA sequences, are similar on different rye chromosomes, are R-genome specific, and can be used to identify rye chromosomes and chromosome fragments in wheat-rye hybrids. Our research widens the application range of chromosome painting in plants.

Authors+Show Affiliations

a Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25429799

Citation

Deng, Chuanliang, et al. "DOP-PCR Based Painting of Rye Chromosomes in a Wheat Background." Genome, vol. 57, no. 9, 2014, pp. 473-9.
Deng C, Bai L, Li S, et al. DOP-PCR based painting of rye chromosomes in a wheat background. Genome. 2014;57(9):473-9.
Deng, C., Bai, L., Li, S., Zhang, Y., Li, X., Chen, Y., Wang, R. R., Han, F., & Hu, Z. (2014). DOP-PCR based painting of rye chromosomes in a wheat background. Genome, 57(9), 473-9. https://doi.org/10.1139/gen-2014-0110
Deng C, et al. DOP-PCR Based Painting of Rye Chromosomes in a Wheat Background. Genome. 2014;57(9):473-9. PubMed PMID: 25429799.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DOP-PCR based painting of rye chromosomes in a wheat background. AU - Deng,Chuanliang, AU - Bai,Lili, AU - Li,Shufen, AU - Zhang,Yingxin, AU - Li,Xiang, AU - Chen,Yuhong, AU - Wang,Richard R-C, AU - Han,Fangpu, AU - Hu,Zanmin, Y1 - 2014/11/03/ PY - 2014/11/29/entrez PY - 2014/11/29/pubmed PY - 2015/8/27/medline KW - 1R KW - 1RS KW - Secale cereale KW - chromosome painting KW - marquage des chromosomes KW - microdissection SP - 473 EP - 9 JF - Genome JO - Genome VL - 57 IS - 9 N2 - To determine the appropriateness of chromosome painting for identifying genomic elements in rye, we microdissected the 1R and 1RS chromosomes from rye (Secale cereale L. var. King II) and wheat-rye addition line 1RS, respectively. Degenerate oligonucleotide primed - polymerase chain reaction (DOP-PCR) amplification of 1R and 1RS products from dissected chromosomes were used as probes to hybridize to metaphase chromosomes of rye, wheat-rye addition lines 1R and 1RS, translocation line 1RS.1BL, and allohexaploid triticale. The results showed that (i) the hybridization signal distribution patterns on rye chromosomes using 1R-derived DOP-PCR products as the probe were similar to those using 1RS-derived DOP-PCR products as the probe; (ii) 1R and (or) 1RS could not be distinguished from other rye chromosomes solely by the hybridization patterns using 1R- and (or) 1RS-derived DOP-PCR products as the probe; (iii) rye chromosomes and (or) rye chromosome fragments could be clearly identified in wheat-rye hybrids using either 1R- or 1RS-derived DOP-PCR products as the probe and could be more accurate in the nontelomeric region than using genomic in situ hybridization (GISH). Our results suggested that 1R- and (or) 1RS-derived DOP-PCR products contain many repetitive DNA sequences, are similar on different rye chromosomes, are R-genome specific, and can be used to identify rye chromosomes and chromosome fragments in wheat-rye hybrids. Our research widens the application range of chromosome painting in plants. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/25429799/DOP_PCR_based_painting_of_rye_chromosomes_in_a_wheat_background_ DB - PRIME DP - Unbound Medicine ER -