In vitro and in vivo protective effect of arginine against lipopolysaccharide induced inflammatory response in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian).
Fish Shellfish Immunol. 2015 Feb; 42(2):457-64.FS

Abstract

The present study was designed to assess the possible protective effects of arginine (Arg) against lipopolysaccharide (LPS) induced inflammatory response in juvenile Jian carp (Cyprinus carpio var. Jian) in vivo and in enterocytes in vitro. Firstly, inflammatory response was established by exposing enterocytes to different concentrations of LPS for 24 h. Secondly, the protective effects of Arg against subsequent LPS exposure were studied in enterocytes. Finally, we investigated whether dietary Arg supplementation could attenuate immune challenge induced by LPS in vivo. The result indicated that 10 mg/L LPS could induced inflammatory response in enterocytes. Cells exposed to LPS (10-30 mg/L) alone for 24 h resulted in a significant increase in lactate dehydrogenase release (LDH) (P < 0.05). The cell viability, protein content, alkaline phosphatase activity were decreased by LPS (P < 0.05). Moreover, LPS exposure significantly increased TNF-α, IL-1β, and IL-6 mRNA expression in vitro (P < 0.05). However, pre-treatment with Arg remarkably prevented the increase of TNF-α, IL-1β, and IL-6 by inhibiting the excessive activation of TLR4-Myd88 signaling pathway through down-regulating TLR4, Myd88, NFκB p65, and MAPK p38 mRNA expression (P < 0.05). Interestingly, the experiment in vivo showed that Arg pre-supplementation could attenuate immune challenge induced by LPS via TLR4-Myd88 signaling pathway, and thus protect fish against LPS-induced inflammatory response. In conclusion, all of these results indicated pre-supplementation with Arg decreased LPS induced immune damage and regulated TLR4-Myd88 signaling pathway in juvenile Jian carp in vivo and in enterocytes in vitro.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Jiang J

College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China.

Shi D

College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China; Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China.

Zhou XQ

Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China.

Hu Y

College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China.

MeSH

Animal FeedAnimalsArginineCarpsDietDietary SupplementsEnterocytesFish DiseasesGene Expression RegulationInflammationIntestinesLipopolysaccharidesRandom Allocation

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25433136

Citation

Jiang, Jun, et al. "In Vitro and in Vivo Protective Effect of Arginine Against Lipopolysaccharide Induced Inflammatory Response in the Intestine of Juvenile Jian Carp (Cyprinus Carpio Var. Jian)." Fish & Shellfish Immunology, vol. 42, no. 2, 2015, pp. 457-64.

Jiang J, Shi D, Zhou XQ, et al. In vitro and in vivo protective effect of arginine against lipopolysaccharide induced inflammatory response in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian). Fish Shellfish Immunol. 2015;42(2):457-64.

Jiang, J., Shi, D., Zhou, X. Q., Hu, Y., Feng, L., Liu, Y., Jiang, W. D., & Zhao, Y. (2015). In vitro and in vivo protective effect of arginine against lipopolysaccharide induced inflammatory response in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian). Fish & Shellfish Immunology, 42(2), 457-64. https://doi.org/10.1016/j.fsi.2014.11.030

Jiang J, et al. In Vitro and in Vivo Protective Effect of Arginine Against Lipopolysaccharide Induced Inflammatory Response in the Intestine of Juvenile Jian Carp (Cyprinus Carpio Var. Jian). Fish Shellfish Immunol. 2015;42(2):457-64. PubMed PMID: 25433136.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - In vitro and in vivo protective effect of arginine against lipopolysaccharide induced inflammatory response in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian). AU - Jiang,Jun, AU - Shi,Dan, AU - Zhou,Xiao-Qiu, AU - Hu,Yi, AU - Feng,Lin, AU - Liu,Yang, AU - Jiang,Wei-Dan, AU - Zhao,Ye, Y1 - 2014/11/27/ PY - 2014/09/28/received PY - 2014/11/19/revised PY - 2014/11/21/accepted PY - 2014/11/30/entrez PY - 2014/11/30/pubmed PY - 2015/9/12/medline KW - Arginine KW - Cyprinus carpio var. Jian KW - Enterocytes KW - Lipopolysaccharide KW - TLR4-Myd88 signaling pathway SP - 457 EP - 64 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 42 IS - 2 N2 - The present study was designed to assess the possible protective effects of arginine (Arg) against lipopolysaccharide (LPS) induced inflammatory response in juvenile Jian carp (Cyprinus carpio var. Jian) in vivo and in enterocytes in vitro. Firstly, inflammatory response was established by exposing enterocytes to different concentrations of LPS for 24 h. Secondly, the protective effects of Arg against subsequent LPS exposure were studied in enterocytes. Finally, we investigated whether dietary Arg supplementation could attenuate immune challenge induced by LPS in vivo. The result indicated that 10 mg/L LPS could induced inflammatory response in enterocytes. Cells exposed to LPS (10-30 mg/L) alone for 24 h resulted in a significant increase in lactate dehydrogenase release (LDH) (P < 0.05). The cell viability, protein content, alkaline phosphatase activity were decreased by LPS (P < 0.05). Moreover, LPS exposure significantly increased TNF-α, IL-1β, and IL-6 mRNA expression in vitro (P < 0.05). However, pre-treatment with Arg remarkably prevented the increase of TNF-α, IL-1β, and IL-6 by inhibiting the excessive activation of TLR4-Myd88 signaling pathway through down-regulating TLR4, Myd88, NFκB p65, and MAPK p38 mRNA expression (P < 0.05). Interestingly, the experiment in vivo showed that Arg pre-supplementation could attenuate immune challenge induced by LPS via TLR4-Myd88 signaling pathway, and thus protect fish against LPS-induced inflammatory response. In conclusion, all of these results indicated pre-supplementation with Arg decreased LPS induced immune damage and regulated TLR4-Myd88 signaling pathway in juvenile Jian carp in vivo and in enterocytes in vitro. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/25433136/In_vitro_and_in_vivo_protective_effect_of_arginine_against_lipopolysaccharide_induced_inflammatory_response_in_the_intestine_of_juvenile_Jian_carp__Cyprinus_carpio_var__Jian__ DB - PRIME DP - Unbound Medicine ER -

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In vitro and in vivo protective effect of arginine against lipopolysaccharide induced inflammatory response in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian).Fish Shellfish Immunol. 2015 Feb; 42(2):457-64.FS