Tags

Type your tag names separated by a space and hit enter

The Changes in the Expression of NF-KB in a Degenerative Human Intervertebral Disc model.
Cell Biochem Biophys. 2015 May; 72(1):115-22.CB

Abstract

We aim at determining the changes in the expression of NF-kB signaling pathway in degenerative intervertebral discs. We collected normal and degenerated intervertebral discs tissues. The normal and degenerated cells were cultivated and their histopathology and immunofluoresence studies were used to observe the position of NF-kB p65 in the cell. We also treated the nucleus pulposus cells with inflammatory factors and inhibitors. Western blot was used to analyze the expression of different proteins. Real time fluorescence-based quantitative PCR was used for observation of NF-kB regulation of change in gene expression. Immunofluorescence showed that in the non-degenerative group the p65 was found in the cytoplasm of the nucleus pulposus cell while in the degenerated cell group the p65 protein was found in the nucleus of the cell. The expression of p65 increased with increase in the degree of degenerative change of the nucleus pulposus cell. RT-PCR showed that the expression of matrix metalloproteinases, aggrecanases and IL-6 was higher in the degenerative group. The amount of aggrecan and type II collagen was significantly decreased in the degenerative group. IL-1β was able to upregulate the activation of NF-kB and the expression of MMP-13 and ADAMTS-4 was also significantly increased. The effect of these proteins can be inhibited by the NF-kB inhibitor, BAY11-7082. The activation of the NK-kB signaling pathway in a degenerative intervertebral disc is gradually increased, regulating the over-expression of matrix-degrading enzymes. It plays an important role in the degradation of extracellular matrix.

Authors+Show Affiliations

Department of Orthopedics Surgery, School of Medicine, Shanghai First People's Hospital Affiliated Shanghai Jiao Tong University, 100, Haining Road, Shanghai, 200080, China.Department of Orthopedics Surgery, School of Medicine, Shanghai First People's Hospital Affiliated Shanghai Jiao Tong University, 100, Haining Road, Shanghai, 200080, China. Spine and Joint Surgery, Central Hospital of Tai An, Shandong, China.Department of Orthopedics Surgery, School of Medicine, Shanghai First People's Hospital Affiliated Shanghai Jiao Tong University, 100, Haining Road, Shanghai, 200080, China.Department of Orthopedics Surgery, School of Medicine, Shanghai First People's Hospital Affiliated Shanghai Jiao Tong University, 100, Haining Road, Shanghai, 200080, China. tjw609@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25433723

Citation

Sun, Zhongyi, et al. "The Changes in the Expression of NF-KB in a Degenerative Human Intervertebral Disc Model." Cell Biochemistry and Biophysics, vol. 72, no. 1, 2015, pp. 115-22.
Sun Z, Yin Z, Liu C, et al. The Changes in the Expression of NF-KB in a Degenerative Human Intervertebral Disc model. Cell Biochem Biophys. 2015;72(1):115-22.
Sun, Z., Yin, Z., Liu, C., & Tian, J. (2015). The Changes in the Expression of NF-KB in a Degenerative Human Intervertebral Disc model. Cell Biochemistry and Biophysics, 72(1), 115-22. https://doi.org/10.1007/s12013-014-0417-3
Sun Z, et al. The Changes in the Expression of NF-KB in a Degenerative Human Intervertebral Disc Model. Cell Biochem Biophys. 2015;72(1):115-22. PubMed PMID: 25433723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Changes in the Expression of NF-KB in a Degenerative Human Intervertebral Disc model. AU - Sun,Zhongyi, AU - Yin,Zhanmin, AU - Liu,Chao, AU - Tian,Jiwei, PY - 2014/12/1/entrez PY - 2014/12/1/pubmed PY - 2016/12/20/medline KW - Intervertebral disc degeneration KW - NF-kB KW - Nucleus pulposus cell KW - Signaling pathway SP - 115 EP - 22 JF - Cell biochemistry and biophysics JO - Cell Biochem. Biophys. VL - 72 IS - 1 N2 - We aim at determining the changes in the expression of NF-kB signaling pathway in degenerative intervertebral discs. We collected normal and degenerated intervertebral discs tissues. The normal and degenerated cells were cultivated and their histopathology and immunofluoresence studies were used to observe the position of NF-kB p65 in the cell. We also treated the nucleus pulposus cells with inflammatory factors and inhibitors. Western blot was used to analyze the expression of different proteins. Real time fluorescence-based quantitative PCR was used for observation of NF-kB regulation of change in gene expression. Immunofluorescence showed that in the non-degenerative group the p65 was found in the cytoplasm of the nucleus pulposus cell while in the degenerated cell group the p65 protein was found in the nucleus of the cell. The expression of p65 increased with increase in the degree of degenerative change of the nucleus pulposus cell. RT-PCR showed that the expression of matrix metalloproteinases, aggrecanases and IL-6 was higher in the degenerative group. The amount of aggrecan and type II collagen was significantly decreased in the degenerative group. IL-1β was able to upregulate the activation of NF-kB and the expression of MMP-13 and ADAMTS-4 was also significantly increased. The effect of these proteins can be inhibited by the NF-kB inhibitor, BAY11-7082. The activation of the NK-kB signaling pathway in a degenerative intervertebral disc is gradually increased, regulating the over-expression of matrix-degrading enzymes. It plays an important role in the degradation of extracellular matrix. SN - 1559-0283 UR - https://www.unboundmedicine.com/medline/citation/25433723/The_Changes_in_the_Expression_of_NF_KB_in_a_Degenerative_Human_Intervertebral_Disc_model_ L2 - https://dx.doi.org/10.1007/s12013-014-0417-3 DB - PRIME DP - Unbound Medicine ER -