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A soybean and fish oil mixture with different n-6/n-3 PUFA ratios modulates the inflammatory reaction in mice with dextran sulfate sodium-induced acute colitis.
Clin Nutr. 2015 Oct; 34(5):1018-24.CN

Abstract

BACKGROUND & AIMS

Inflammatory bowel disease is a recurrent disease of the gastrointestinal tract. n-3 polyunsaturated fatty acids (PUFAs) are proved to have anti-inflammatory and immunomodulatory properties. This study evaluated the effects of different dietary n-6/n-3 PUFA ratios on the mechanism of alleviating the inflammatory response in mice with dextran sulfate sodium (DSS)-induced colitis.

METHODS

Mice were randomly assigned to 6 groups including 3 non-colitis groups (C, LF, and HF) and 3 colitis groups (DC, DLF, and DHF). Mice in the C and DC groups were fed a common semipurified diet with soybean oil as the fat source. The other groups received an identical component except that part of the soybean oil was replaced by different amounts of fish oil. The n-6/n-3 PUFA ratio of the LF and DLF groups was 4:1, the ratio of the HF and DHF groups was 2:1. After feeding the respective diets for 2 weeks, the colitis groups were given distilled water containing 2% DSS, while the non-colitis groups were given distilled water for 5 days. After that, all mice were sacrificed at the recovery phase after drinking distilled water for another 5 days.

RESULTS

Colitis resulted in higher expressions of colonic inflammatory mediators in colon tissues and colon lavage fluid. Also, colonic peroxisome proliferator-activated receptor (PPAR)-γ and the IκBα/nuclear factor (NF)-κB p65 ratio were lower than those of the non-colitis groups. Compared to the DC group, fish oil-enriched colitis groups had lower inflammatory mediator expressions and higher PPAR-γ protein levels and IκBα/NF-κB p65 ratios in colon tissues. The DHF group had even lower colonic inflammatory gene and higher PPAR-γ protein expressions than did the DLF group.

CONCLUSIONS

These findings suggest that diets enriched with fish oil upregulated PPAR-γ and decreased NF-κB activation that may consequently have reduced luminal inflammatory mediator production. Compared to a n-6/n-3 PUFA ratio 4:1, a ratio of 2:1 was more effective in reducing inflammatory reactions in DSS-induced colitis.

Authors+Show Affiliations

School of Nutrition and Health Sciences, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan.Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.Department of Nutrition and Health Science, Chinese Culture University, Taipei, Taiwan.School of Nutrition and Health Sciences, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan. Electronic address: sangling@tmu.edu.tw.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25434577

Citation

Huang, Cyoung-Huei, et al. "A Soybean and Fish Oil Mixture With Different N-6/n-3 PUFA Ratios Modulates the Inflammatory Reaction in Mice With Dextran Sulfate Sodium-induced Acute Colitis." Clinical Nutrition (Edinburgh, Scotland), vol. 34, no. 5, 2015, pp. 1018-24.
Huang CH, Hou YC, Yeh CL, et al. A soybean and fish oil mixture with different n-6/n-3 PUFA ratios modulates the inflammatory reaction in mice with dextran sulfate sodium-induced acute colitis. Clin Nutr. 2015;34(5):1018-24.
Huang, C. H., Hou, Y. C., Yeh, C. L., & Yeh, S. L. (2015). A soybean and fish oil mixture with different n-6/n-3 PUFA ratios modulates the inflammatory reaction in mice with dextran sulfate sodium-induced acute colitis. Clinical Nutrition (Edinburgh, Scotland), 34(5), 1018-24. https://doi.org/10.1016/j.clnu.2014.11.008
Huang CH, et al. A Soybean and Fish Oil Mixture With Different N-6/n-3 PUFA Ratios Modulates the Inflammatory Reaction in Mice With Dextran Sulfate Sodium-induced Acute Colitis. Clin Nutr. 2015;34(5):1018-24. PubMed PMID: 25434577.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A soybean and fish oil mixture with different n-6/n-3 PUFA ratios modulates the inflammatory reaction in mice with dextran sulfate sodium-induced acute colitis. AU - Huang,Cyoung-Huei, AU - Hou,Yu-Chen, AU - Yeh,Chiu-Li, AU - Yeh,Sung-Ling, Y1 - 2014/11/18/ PY - 2014/08/26/received PY - 2014/10/22/revised PY - 2014/11/09/accepted PY - 2014/12/2/entrez PY - 2014/12/2/pubmed PY - 2016/6/4/medline KW - Colitis KW - Inflammatory mediator KW - NF-κB KW - PPAR-γ KW - n-3 PUFA SP - 1018 EP - 24 JF - Clinical nutrition (Edinburgh, Scotland) JO - Clin Nutr VL - 34 IS - 5 N2 - BACKGROUND & AIMS: Inflammatory bowel disease is a recurrent disease of the gastrointestinal tract. n-3 polyunsaturated fatty acids (PUFAs) are proved to have anti-inflammatory and immunomodulatory properties. This study evaluated the effects of different dietary n-6/n-3 PUFA ratios on the mechanism of alleviating the inflammatory response in mice with dextran sulfate sodium (DSS)-induced colitis. METHODS: Mice were randomly assigned to 6 groups including 3 non-colitis groups (C, LF, and HF) and 3 colitis groups (DC, DLF, and DHF). Mice in the C and DC groups were fed a common semipurified diet with soybean oil as the fat source. The other groups received an identical component except that part of the soybean oil was replaced by different amounts of fish oil. The n-6/n-3 PUFA ratio of the LF and DLF groups was 4:1, the ratio of the HF and DHF groups was 2:1. After feeding the respective diets for 2 weeks, the colitis groups were given distilled water containing 2% DSS, while the non-colitis groups were given distilled water for 5 days. After that, all mice were sacrificed at the recovery phase after drinking distilled water for another 5 days. RESULTS: Colitis resulted in higher expressions of colonic inflammatory mediators in colon tissues and colon lavage fluid. Also, colonic peroxisome proliferator-activated receptor (PPAR)-γ and the IκBα/nuclear factor (NF)-κB p65 ratio were lower than those of the non-colitis groups. Compared to the DC group, fish oil-enriched colitis groups had lower inflammatory mediator expressions and higher PPAR-γ protein levels and IκBα/NF-κB p65 ratios in colon tissues. The DHF group had even lower colonic inflammatory gene and higher PPAR-γ protein expressions than did the DLF group. CONCLUSIONS: These findings suggest that diets enriched with fish oil upregulated PPAR-γ and decreased NF-κB activation that may consequently have reduced luminal inflammatory mediator production. Compared to a n-6/n-3 PUFA ratio 4:1, a ratio of 2:1 was more effective in reducing inflammatory reactions in DSS-induced colitis. SN - 1532-1983 UR - https://www.unboundmedicine.com/medline/citation/25434577/A_soybean_and_fish_oil_mixture_with_different_n_6/n_3_PUFA_ratios_modulates_the_inflammatory_reaction_in_mice_with_dextran_sulfate_sodium_induced_acute_colitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0261-5614(14)00289-1 DB - PRIME DP - Unbound Medicine ER -