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Molecular cytogenetic characterization and stem rust resistance of five wheat-Thinopyrum ponticum partial amphiploids.
J Genet Genomics. 2014 Nov 20; 41(11):591-9.JG

Abstract

Partial amphiploids created by crossing common wheat (Triticum aestivum L.) and Thinopyrum ponticum (Podp.) Barkworth & D. R. Dewey are important intermediates in wheat breeding because of their resistance to major wheat diseases. In this study, we examined the chromosome compositions of five Xiaoyan-series wheat-Th. ponticum partial amphiploids (Xiaoyan 68, Xiaoyan 693, Xiaoyan 784, Xiaoyan 7430, and Xiaoyan 7631) using GISH, multicolor-GISH, and multicolor-FISH. We found several chromosome changes in these lines. For example, wheat chromosomes 1B and 2B were added in Xiaoyan 68 and Xiaoyan 7430, respectively, while wheat chromosome 6B was eliminated from Xiaoyan 693 and Xiaoyan 7631. Chromosome rearrangements were also detected in these amphiploids, including an interspecific translocation involving chromosome 4D and some intergenomic translocations, such as A-B and A-D translocations, among wheat genomes. Analysis of the Th. ponticum chromosomes in the amphiploids showed that some lines shared the same alien chromosomes. We also evaluated these partial amphiploids for resistance to nine races of stem rust, including TTKSK (commonly known as Ug99). Three lines, Xiaoyan 68, Xiaoyan 784, and Xiaoyan 7430, exhibited excellent resistance to all nine races, and could therefore be valuable sources of stem rust resistance in wheat breeding.

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Publisher Full Text (DOI)

Authors+Show Affiliations

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

United States Department of Agriculture, Agricultural Research Service, Cereal Crops Research Unit, Fargo, ND 58102-2765, USA.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

United States Department of Agriculture, Agricultural Research Service, Cereal Crops Research Unit, Fargo, ND 58102-2765, USA.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: zsli@genetics.ac.cn.

MeSH

BasidiomycotaChromosomes, PlantCytogenetic AnalysisDisease ResistanceHybridization, GeneticIn Situ HybridizationPlant DiseasesPloidiesPoaceaeTranslocation, GeneticTriticum

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25434682

Citation

Zheng, Qi, et al. "Molecular Cytogenetic Characterization and Stem Rust Resistance of Five wheat-Thinopyrum Ponticum Partial Amphiploids." Journal of Genetics and Genomics = Yi Chuan Xue Bao, vol. 41, no. 11, 2014, pp. 591-9.

Zheng Q, Lv Z, Niu Z, et al. Molecular cytogenetic characterization and stem rust resistance of five wheat-Thinopyrum ponticum partial amphiploids. J Genet Genomics. 2014;41(11):591-9.

Zheng, Q., Lv, Z., Niu, Z., Li, B., Li, H., Xu, S. S., Han, F., & Li, Z. (2014). Molecular cytogenetic characterization and stem rust resistance of five wheat-Thinopyrum ponticum partial amphiploids. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 41(11), 591-9. https://doi.org/10.1016/j.jgg.2014.06.003

Zheng Q, et al. Molecular Cytogenetic Characterization and Stem Rust Resistance of Five wheat-Thinopyrum Ponticum Partial Amphiploids. J Genet Genomics. 2014 Nov 20;41(11):591-9. PubMed PMID: 25434682.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Molecular cytogenetic characterization and stem rust resistance of five wheat-Thinopyrum ponticum partial amphiploids. AU - Zheng,Qi, AU - Lv,Zhenling, AU - Niu,Zhixia, AU - Li,Bin, AU - Li,Hongwei, AU - Xu,Steven S, AU - Han,Fangpu, AU - Li,Zhensheng, Y1 - 2014/06/14/ PY - 2014/01/24/received PY - 2014/05/27/revised PY - 2014/06/09/accepted PY - 2014/12/2/entrez PY - 2014/12/2/pubmed PY - 2015/7/21/medline KW - Multicolor-fluorescent in situ hybridization (mc-FISH) KW - Multicolor-genomic in situ hybridization (mc-GISH) KW - Partial amphiploid KW - Stem rust KW - Thinopyrum ponticum KW - Triticum aestivum SP - 591 EP - 9 JF - Journal of genetics and genomics = Yi chuan xue bao JO - J Genet Genomics VL - 41 IS - 11 N2 - Partial amphiploids created by crossing common wheat (Triticum aestivum L.) and Thinopyrum ponticum (Podp.) Barkworth & D. R. Dewey are important intermediates in wheat breeding because of their resistance to major wheat diseases. In this study, we examined the chromosome compositions of five Xiaoyan-series wheat-Th. ponticum partial amphiploids (Xiaoyan 68, Xiaoyan 693, Xiaoyan 784, Xiaoyan 7430, and Xiaoyan 7631) using GISH, multicolor-GISH, and multicolor-FISH. We found several chromosome changes in these lines. For example, wheat chromosomes 1B and 2B were added in Xiaoyan 68 and Xiaoyan 7430, respectively, while wheat chromosome 6B was eliminated from Xiaoyan 693 and Xiaoyan 7631. Chromosome rearrangements were also detected in these amphiploids, including an interspecific translocation involving chromosome 4D and some intergenomic translocations, such as A-B and A-D translocations, among wheat genomes. Analysis of the Th. ponticum chromosomes in the amphiploids showed that some lines shared the same alien chromosomes. We also evaluated these partial amphiploids for resistance to nine races of stem rust, including TTKSK (commonly known as Ug99). Three lines, Xiaoyan 68, Xiaoyan 784, and Xiaoyan 7430, exhibited excellent resistance to all nine races, and could therefore be valuable sources of stem rust resistance in wheat breeding. SN - 1673-8527 UR - https://www.unboundmedicine.com/medline/citation/25434682/Molecular_cytogenetic_characterization_and_stem_rust_resistance_of_five_wheat_Thinopyrum_ponticum_partial_amphiploids_ DB - PRIME DP - Unbound Medicine ER -