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Hepatoprotective properties of aqueous leaf extract of Persea Americana, Mill (Lauraceae) 'avocado' against CCL4-induced damage in rats.

Abstract

BACKGROUND

Natural products from plants have received considerable attention in recent years due to their diverse pharmacological properties, including antioxidants and hepatoprotective activities. The protective effects of aqueous extract of Persea americana (AEPA) against carbon tetrachloride (CCl4)-induced hepatotoxicity in male albino rats was investigated.

MATERIALS AND METHODS

Liver damage was induced in rats by administering a 1:1 (v/v) mixture of CCl4 and olive oil [3 ml/kg, subcutaneously (sc)] after pre-treatment for 7 days with AEPA. Hepatoprotective effects of AEPA was evaluated by estimating the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and levels of total bilirubin (TBL). The effects of AEPA on biomarkers of oxidative damage (lipid peroxidation) and antioxidant enzymes namely, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione S-transferase (GST) were measured in liver post mitochondrial fraction.

RESULTS

AEPA and Reducdyn® showed significant (p<0.05) hepatoprotective activity by decreasing the activities of ALT, AST, ALP and reducing the levels of TBL. The activities of antioxidant enzymes, levels of malondialdehyde and protein carbonyls were also decreased dose-dependently in the AEPA-treated rats. Pre-treatment with AEPA also decreased the serum levels of glutathione significantly.

CONCLUSION

These data revealed that AEPA possesses significant hepatoprotective effects against CCl4-induced toxicity attributable to its constituent phytochemicals. The mechanism of hepatoprotection seems to be through modulation of antioxidant enzyme system.

Authors+Show Affiliations

Nigerian Institute of Medical Research, P. M. B. 2013, Yaba, Lagos. Nigeria.Laboratories for Biomembrane and Cancer Research, Department of Biochemistry, College of Medicine, University of Lagos, Nigeria.C/O Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan. Nigeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25435602

Citation

Brai, Bartholomew I C., et al. "Hepatoprotective Properties of Aqueous Leaf Extract of Persea Americana, Mill (Lauraceae) 'avocado' Against CCL4-induced Damage in Rats." African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, vol. 11, no. 2, 2014, pp. 237-44.
Brai BI, Adisa RA, Odetola AA. Hepatoprotective properties of aqueous leaf extract of Persea Americana, Mill (Lauraceae) 'avocado' against CCL4-induced damage in rats. Afr J Tradit Complement Altern Med. 2014;11(2):237-44.
Brai, B. I., Adisa, R. A., & Odetola, A. A. (2014). Hepatoprotective properties of aqueous leaf extract of Persea Americana, Mill (Lauraceae) 'avocado' against CCL4-induced damage in rats. African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, 11(2), pp. 237-44.
Brai BI, Adisa RA, Odetola AA. Hepatoprotective Properties of Aqueous Leaf Extract of Persea Americana, Mill (Lauraceae) 'avocado' Against CCL4-induced Damage in Rats. Afr J Tradit Complement Altern Med. 2014;11(2):237-44. PubMed PMID: 25435602.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective properties of aqueous leaf extract of Persea Americana, Mill (Lauraceae) 'avocado' against CCL4-induced damage in rats. AU - Brai,Bartholomew I C, AU - Adisa,Rahmat A, AU - Odetola,Adebimpe A, Y1 - 2014/01/28/ PY - 2014/12/2/entrez PY - 2014/12/2/pubmed PY - 2015/7/15/medline KW - Persea americana KW - antioxidants enzymes KW - carbon tetrachloride KW - hepatotoxicity KW - lipid peroxidation SP - 237 EP - 44 JF - African journal of traditional, complementary, and alternative medicines : AJTCAM JO - Afr J Tradit Complement Altern Med VL - 11 IS - 2 N2 - BACKGROUND: Natural products from plants have received considerable attention in recent years due to their diverse pharmacological properties, including antioxidants and hepatoprotective activities. The protective effects of aqueous extract of Persea americana (AEPA) against carbon tetrachloride (CCl4)-induced hepatotoxicity in male albino rats was investigated. MATERIALS AND METHODS: Liver damage was induced in rats by administering a 1:1 (v/v) mixture of CCl4 and olive oil [3 ml/kg, subcutaneously (sc)] after pre-treatment for 7 days with AEPA. Hepatoprotective effects of AEPA was evaluated by estimating the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and levels of total bilirubin (TBL). The effects of AEPA on biomarkers of oxidative damage (lipid peroxidation) and antioxidant enzymes namely, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione S-transferase (GST) were measured in liver post mitochondrial fraction. RESULTS: AEPA and Reducdyn® showed significant (p<0.05) hepatoprotective activity by decreasing the activities of ALT, AST, ALP and reducing the levels of TBL. The activities of antioxidant enzymes, levels of malondialdehyde and protein carbonyls were also decreased dose-dependently in the AEPA-treated rats. Pre-treatment with AEPA also decreased the serum levels of glutathione significantly. CONCLUSION: These data revealed that AEPA possesses significant hepatoprotective effects against CCl4-induced toxicity attributable to its constituent phytochemicals. The mechanism of hepatoprotection seems to be through modulation of antioxidant enzyme system. SN - 2505-0044 UR - https://www.unboundmedicine.com/medline/citation/25435602/Hepatoprotective_properties_of_aqueous_leaf_extract_of_Persea_Americana_Mill__Lauraceae__'avocado'_against_CCL4_induced_damage_in_rats_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25435602/ DB - PRIME DP - Unbound Medicine ER -