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Macrophage migration inhibitory factor as a potential biomarker of endometriosis.
Fertil Steril. 2015 Jan; 103(1):153-9.e3.FS

Abstract

OBJECTIVE

To evaluate the expression of MIF, CD74, and COX-2 in normal, ectopic, and eutopic endometrium during the menstrual cycle and to assess MIF level in peripheral blood.

DESIGN

The expressions of MIF, CD74, and COX-2 in normal, ectopic, and eutopic endometrium were evaluated with the use of real-time polymerase chain reaction. MIF protein in peripheral blood samples was checked with the use of ELISA.

SETTING

Reproductive biomedicine research center.

PATIENT(S)

Sixteen normal women and 20 women with endometriosis.

INTERVENTION(S)

Ectopic biopsies were obtained with the use of laparoscopic procedure, and eutopic and control biopsies were obtained with the use of Pipelle. Peripheral blood samples were collected before laparoscopy.

MAIN OUTCOME MEASURE(S)

The expression of MIF, CD74, and COX-2 in normal, ectopic and eutopic endometrium during the menstrual cycle and the expression level of MIF in peripheral blood samples.

RESULT(S)

Relative mRNA expression of MIF, CD74, and COX-2 were significantly higher in ectopic endometrium than in eutopic and control endometrium. Also, there were significant differences in expression of these genes in normal, ectopic, and eutopic endometrium during the menstrual cycle. Moreover, women with endometriosis had significantly higher circulating levels of MIF compared with control subjects.

CONCLUSION(S)

Dynamic expression of MIF, CD74, and COX-2 during the menstrual cycle could play an essential role in reproduction, inflammation, and endometrium reconstruction. A higher expression of these genes in ectopic endometrium can be considered as a molecular biomarker for endometriosis development and pathophysiology. Also, a high level of MIF in blood serum can act as a biomarker in the diagnosis of endometriosis.

Authors+Show Affiliations

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran; Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education Culture and Research, Tehran, Iran.Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education Culture and Research, Tehran, Iran.Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education Culture and Research, Tehran, Iran.Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education Culture and Research, Tehran, Iran.Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education Culture and Research, Tehran, Iran.Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education Culture and Research, Tehran, Iran. Electronic address: m.shahhoseini@royaninstitute.org.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25439837

Citation

Mahdian, Soodeh, et al. "Macrophage Migration Inhibitory Factor as a Potential Biomarker of Endometriosis." Fertility and Sterility, vol. 103, no. 1, 2015, pp. 153-9.e3.
Mahdian S, Aflatoonian R, Yazdi RS, et al. Macrophage migration inhibitory factor as a potential biomarker of endometriosis. Fertil Steril. 2015;103(1):153-9.e3.
Mahdian, S., Aflatoonian, R., Yazdi, R. S., Yaghmaei, P., Ramazanali, F., Afsharian, P., & Shahhoseini, M. (2015). Macrophage migration inhibitory factor as a potential biomarker of endometriosis. Fertility and Sterility, 103(1), 153-e3. https://doi.org/10.1016/j.fertnstert.2014.09.031
Mahdian S, et al. Macrophage Migration Inhibitory Factor as a Potential Biomarker of Endometriosis. Fertil Steril. 2015;103(1):153-9.e3. PubMed PMID: 25439837.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Macrophage migration inhibitory factor as a potential biomarker of endometriosis. AU - Mahdian,Soodeh, AU - Aflatoonian,Reza, AU - Yazdi,Reza Salman, AU - Yaghmaei,Parichehr, AU - Ramazanali,Fariba, AU - Afsharian,Parvaneh, AU - Shahhoseini,Maryam, Y1 - 2014/10/28/ PY - 2014/06/28/received PY - 2014/09/20/revised PY - 2014/09/23/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/4/15/medline KW - CD74 KW - COX-2 KW - MIF KW - endometriosis SP - 153 EP - 9.e3 JF - Fertility and sterility JO - Fertil. Steril. VL - 103 IS - 1 N2 - OBJECTIVE: To evaluate the expression of MIF, CD74, and COX-2 in normal, ectopic, and eutopic endometrium during the menstrual cycle and to assess MIF level in peripheral blood. DESIGN: The expressions of MIF, CD74, and COX-2 in normal, ectopic, and eutopic endometrium were evaluated with the use of real-time polymerase chain reaction. MIF protein in peripheral blood samples was checked with the use of ELISA. SETTING: Reproductive biomedicine research center. PATIENT(S): Sixteen normal women and 20 women with endometriosis. INTERVENTION(S): Ectopic biopsies were obtained with the use of laparoscopic procedure, and eutopic and control biopsies were obtained with the use of Pipelle. Peripheral blood samples were collected before laparoscopy. MAIN OUTCOME MEASURE(S): The expression of MIF, CD74, and COX-2 in normal, ectopic and eutopic endometrium during the menstrual cycle and the expression level of MIF in peripheral blood samples. RESULT(S): Relative mRNA expression of MIF, CD74, and COX-2 were significantly higher in ectopic endometrium than in eutopic and control endometrium. Also, there were significant differences in expression of these genes in normal, ectopic, and eutopic endometrium during the menstrual cycle. Moreover, women with endometriosis had significantly higher circulating levels of MIF compared with control subjects. CONCLUSION(S): Dynamic expression of MIF, CD74, and COX-2 during the menstrual cycle could play an essential role in reproduction, inflammation, and endometrium reconstruction. A higher expression of these genes in ectopic endometrium can be considered as a molecular biomarker for endometriosis development and pathophysiology. Also, a high level of MIF in blood serum can act as a biomarker in the diagnosis of endometriosis. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/25439837/Macrophage_migration_inhibitory_factor_as_a_potential_biomarker_of_endometriosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(14)02208-0 DB - PRIME DP - Unbound Medicine ER -