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A novel reliable method of DNA extraction from olive oil suitable for molecular traceability.
Food Chem. 2015 Apr 01; 172:596-602.FC

Abstract

Extra virgin olive oil production has a worldwide economic impact. The use of this brand, however, is of great concern to Institutions and private industries because of the increasing number of fraud and adulteration attempts to the market products. Here, we present a novel, reliable and not expensive method for extracting the DNA from commercial virgin and extra virgin olive oils. The DNA is stable overtime and amenable for molecular analyses; in fact, by carrying out simple sequence repeats (SSRs) markers analysis, we characterise the genetic profile of monovarietal olive oils. By comparing the oil-derived pattern with that of the corresponding tree, we can unambiguously identify four cultivars from Samnium, a region of Southern Italy, and distinguish them from reference and more widely used varieties. Through a parentage statistical analysis, we also identify the putative pollinators, establishing an unprecedented and powerful tool for olive oil traceability.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Raieta K
Department of Sciences and Technologies, University of Sannio, 82100 Benevento, Italy.
Muccillo L
Department of Sciences and Technologies, University of Sannio, 82100 Benevento, Italy.
Colantuoni V
Department of Sciences and Technologies, University of Sannio, 82100 Benevento, Italy. Electronic address: colantuoni@unisannio.it.

MeSH

DNA, PlantItalyMicrosatellite RepeatsOleaOlive OilPlant Oils

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25442596

Citation

Raieta, Katia, et al. "A Novel Reliable Method of DNA Extraction From Olive Oil Suitable for Molecular Traceability." Food Chemistry, vol. 172, 2015, pp. 596-602.
Raieta K, Muccillo L, Colantuoni V. A novel reliable method of DNA extraction from olive oil suitable for molecular traceability. Food Chem. 2015;172:596-602.
Raieta, K., Muccillo, L., & Colantuoni, V. (2015). A novel reliable method of DNA extraction from olive oil suitable for molecular traceability. Food Chemistry, 172, 596-602. https://doi.org/10.1016/j.foodchem.2014.09.101
Raieta K, Muccillo L, Colantuoni V. A Novel Reliable Method of DNA Extraction From Olive Oil Suitable for Molecular Traceability. Food Chem. 2015 Apr 1;172:596-602. PubMed PMID: 25442596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel reliable method of DNA extraction from olive oil suitable for molecular traceability. AU - Raieta,Katia, AU - Muccillo,Livio, AU - Colantuoni,Vittorio, Y1 - 2014/09/28/ PY - 2014/02/19/received PY - 2014/08/04/revised PY - 2014/09/17/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/7/7/medline KW - Cultivar authentication KW - DNA extraction KW - Olive oil KW - SSR analysis KW - Traceability SP - 596 EP - 602 JF - Food chemistry JO - Food Chem VL - 172 N2 - Extra virgin olive oil production has a worldwide economic impact. The use of this brand, however, is of great concern to Institutions and private industries because of the increasing number of fraud and adulteration attempts to the market products. Here, we present a novel, reliable and not expensive method for extracting the DNA from commercial virgin and extra virgin olive oils. The DNA is stable overtime and amenable for molecular analyses; in fact, by carrying out simple sequence repeats (SSRs) markers analysis, we characterise the genetic profile of monovarietal olive oils. By comparing the oil-derived pattern with that of the corresponding tree, we can unambiguously identify four cultivars from Samnium, a region of Southern Italy, and distinguish them from reference and more widely used varieties. Through a parentage statistical analysis, we also identify the putative pollinators, establishing an unprecedented and powerful tool for olive oil traceability. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/25442596/A_novel_reliable_method_of_DNA_extraction_from_olive_oil_suitable_for_molecular_traceability_ DB - PRIME DP - Unbound Medicine ER -