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Evaluation of antifungal activities of the essential oil and various extracts of Nigella sativa and its main component, thymoquinone against pathogenic dermatophyte strains.
J Mycol Med. 2014 Dec; 24(4):e155-61.JM

Abstract

OBJECTIVE

Plant extracts and plant-derived compounds are valuable sources as folk medicine for the treatment and prevention of a wide range of diseases including infectious diseases. In the present study, the antifungal activities of the essential oil and various extracts Nigella sativa and its active principle, thymoquinone against Trichophyton mentagrophytes, Microsporum canis and Microsporum gypseum as pathogenic dermatophyte strains have been evaluated. In addition, the cytotoxic effects of N. sativa against murine macrophage cells were determined.

MATERIALS AND METHODS

In this study, the antifungal activity was studied by disk diffusion method and assessment of minimum inhibitory concentration (MIC) of extracts using broth macrodilution method. In addition, the cytotoxic activity of N. sativa was evaluated by colorimetric assay (MTT). The components of the N. sativa essential oil were also identified by gas chromatography/mass spectroscopy (GC/MS) analysis.

RESULTS

The results showed that the essential oil and various extracts of N. sativa particularly thymoquinone have potent antifungal effects on T. mentagrophytes, M. canis and M. gypseum as pathogenic dermatophyte strains. In the assessment of the cytotoxicity activity, it could be observed that N. sativa had no significant cytotoxicity in the murine macrophages at low concentrations. While, thymoquinone in comparison with essential oil and various extracts of N. sativa showed higher cytotoxicity on murine macrophage cells. In the GC/MS analysis, thymoquinone (42.4%), p-cymene (14.1%), carvacrol (10.3%) and longifolene (6.1%) were found to be the major components of N. sativa essential oil.

CONCLUSION

The findings of this study suggest a first step in the search of new antidermatophytic drugs and aid the use of N. sativa seeds in the traditional medicine for dermatophytic infections.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Mahmoudvand H
Research Center for Tropical and Infectious Diseases, Kerman University of Medical Science, Kerman, Iran; Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.
Sepahvand A
Department of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran.
Jahanbakhsh S
Research Center for Tropical and Infectious Diseases, Kerman University of Medical Science, Kerman, Iran; Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.
Ezatpour B
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Ayatollahi Mousavi SA
Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran. Electronic address: aminayatollahi@kmu.ac.ir.

MeSH

AnimalsAntifungal AgentsArthrodermataceaeBenzoquinonesCells, CulturedDermatomycosesDrug Evaluation, PreclinicalMaleMiceMice, Inbred BALB CMicrobial Sensitivity TestsNigella sativaOils, VolatilePlant ExtractsPlant Oils

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25442918

Citation

Mahmoudvand, H, et al. "Evaluation of Antifungal Activities of the Essential Oil and Various Extracts of Nigella Sativa and Its Main Component, Thymoquinone Against Pathogenic Dermatophyte Strains." Journal De Mycologie Medicale, vol. 24, no. 4, 2014, pp. e155-61.
Mahmoudvand H, Sepahvand A, Jahanbakhsh S, et al. Evaluation of antifungal activities of the essential oil and various extracts of Nigella sativa and its main component, thymoquinone against pathogenic dermatophyte strains. J Mycol Med. 2014;24(4):e155-61.
Mahmoudvand, H., Sepahvand, A., Jahanbakhsh, S., Ezatpour, B., & Ayatollahi Mousavi, S. A. (2014). Evaluation of antifungal activities of the essential oil and various extracts of Nigella sativa and its main component, thymoquinone against pathogenic dermatophyte strains. Journal De Mycologie Medicale, 24(4), e155-61. https://doi.org/10.1016/j.mycmed.2014.06.048
Mahmoudvand H, et al. Evaluation of Antifungal Activities of the Essential Oil and Various Extracts of Nigella Sativa and Its Main Component, Thymoquinone Against Pathogenic Dermatophyte Strains. J Mycol Med. 2014;24(4):e155-61. PubMed PMID: 25442918.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of antifungal activities of the essential oil and various extracts of Nigella sativa and its main component, thymoquinone against pathogenic dermatophyte strains. AU - Mahmoudvand,H, AU - Sepahvand,A, AU - Jahanbakhsh,S, AU - Ezatpour,B, AU - Ayatollahi Mousavi,S A, Y1 - 2014/10/19/ PY - 2013/08/20/received PY - 2014/05/12/revised PY - 2014/06/26/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2016/1/30/medline KW - Black cumin KW - Microsporum canis KW - Microsporum gypseum KW - Nigella sativa KW - Thymoquinone KW - Trichophyton mentagrophytes SP - e155 EP - 61 JF - Journal de mycologie medicale JO - J Mycol Med VL - 24 IS - 4 N2 - OBJECTIVE: Plant extracts and plant-derived compounds are valuable sources as folk medicine for the treatment and prevention of a wide range of diseases including infectious diseases. In the present study, the antifungal activities of the essential oil and various extracts Nigella sativa and its active principle, thymoquinone against Trichophyton mentagrophytes, Microsporum canis and Microsporum gypseum as pathogenic dermatophyte strains have been evaluated. In addition, the cytotoxic effects of N. sativa against murine macrophage cells were determined. MATERIALS AND METHODS: In this study, the antifungal activity was studied by disk diffusion method and assessment of minimum inhibitory concentration (MIC) of extracts using broth macrodilution method. In addition, the cytotoxic activity of N. sativa was evaluated by colorimetric assay (MTT). The components of the N. sativa essential oil were also identified by gas chromatography/mass spectroscopy (GC/MS) analysis. RESULTS: The results showed that the essential oil and various extracts of N. sativa particularly thymoquinone have potent antifungal effects on T. mentagrophytes, M. canis and M. gypseum as pathogenic dermatophyte strains. In the assessment of the cytotoxicity activity, it could be observed that N. sativa had no significant cytotoxicity in the murine macrophages at low concentrations. While, thymoquinone in comparison with essential oil and various extracts of N. sativa showed higher cytotoxicity on murine macrophage cells. In the GC/MS analysis, thymoquinone (42.4%), p-cymene (14.1%), carvacrol (10.3%) and longifolene (6.1%) were found to be the major components of N. sativa essential oil. CONCLUSION: The findings of this study suggest a first step in the search of new antidermatophytic drugs and aid the use of N. sativa seeds in the traditional medicine for dermatophytic infections. SN - 1773-0449 UR - https://www.unboundmedicine.com/medline/citation/25442918/Evaluation_of_antifungal_activities_of_the_essential_oil_and_various_extracts_of_Nigella_sativa_and_its_main_component_thymoquinone_against_pathogenic_dermatophyte_strains_ DB - PRIME DP - Unbound Medicine ER -