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Characterization of tetracycline and methicillin resistant Staphylococcus aureus strains in a Spanish hospital: is livestock-contact a risk factor in infections caused by MRSA CC398?
Int J Med Microbiol. 2014 Nov; 304(8):1226-32.IJ

Abstract

Tetracycline-resistance (Tet(R)) has been postulated as a marker of the livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) lineage CC398.

OBJECTIVES OF THE STUDY

to determine the spa-types and assigned MLST clonal complexes (CCs) among all 98 MRSA-Tet(R) strains recovered during 2011-2012 (from different patients) in a Spanish Hospital, analyzing the possible correlation with livestock-contact of the patients. All 98 strains were assigned to 9 CCs: CC398 (60.2%), CC1 (19.4%), CC5 (12.2%), and other CCs (8.2%). The 98 patients were classified into three groups: (A) contact with livestock-animals (n=25); (B) no-contact with livestock-animals (n=42); (C) no information about animal contact (n=31). A significant higher percentage of CC398 strains was obtained in group A (76%) than in group B (50%) (p<0.05), being the percentage in group C of 61.3%. Most of MRSA-Tet(R)-CC398 strains presented a multi-resistance phenotype, including erythromycin, clindamycin, and ciprofloxacin, and the most prevalent detected genes were tet(M) and erm(C). Three strains presented the phenotype macrolide-susceptibility/lincosamide-resistance and contained the vga(A) gene. MRSA-CC1 strains showed higher percentages of erythromycin/clindamycin resistance (95%/89%) than MRSA-CC398 strains (58%/63%), and this resistance was usually mediated by erm(C) gene. Most of MRSA-CC5 strains showed resistance to ciprofloxacin, tobramycin/kanamycin and erythromycin. None of the strains presented the genes lukF/lukS-PV, tsst-1, eta, etb or etd. All MRSA-CC398 strains lacked the genes of the immune-evasion-cluster, but MRSA-CC1 strains carried these genes (type E). In conclusion, although MRSA CC398 is detected in a significant higher proportion in patients with livestock-contact; its detection in people without this type of contact also indicates its capacity for human-to-human transmission.

Authors+Show Affiliations

Area de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Area de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Servicio de Microbiología, Hospital Universitario Miguel Servet, IIS Aragón, Zaragoza, Spain; Universidad de Zaragoza, Zaragoza, Spain.Servicio de Microbiología, Hospital Universitario Miguel Servet, IIS Aragón, Zaragoza, Spain.Servicio de Microbiología, Hospital Universitario Miguel Servet, IIS Aragón, Zaragoza, Spain.Area de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Area de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Servicio de Microbiología, Hospital Universitario Miguel Servet, IIS Aragón, Zaragoza, Spain.Area de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain. Electronic address: carmen.torres@unirioja.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25444568

Citation

Benito, Daniel, et al. "Characterization of Tetracycline and Methicillin Resistant Staphylococcus Aureus Strains in a Spanish Hospital: Is Livestock-contact a Risk Factor in Infections Caused By MRSA CC398?" International Journal of Medical Microbiology : IJMM, vol. 304, no. 8, 2014, pp. 1226-32.
Benito D, Lozano C, Rezusta A, et al. Characterization of tetracycline and methicillin resistant Staphylococcus aureus strains in a Spanish hospital: is livestock-contact a risk factor in infections caused by MRSA CC398? Int J Med Microbiol. 2014;304(8):1226-32.
Benito, D., Lozano, C., Rezusta, A., Ferrer, I., Vasquez, M. A., Ceballos, S., Zarazaga, M., Revillo, M. J., & Torres, C. (2014). Characterization of tetracycline and methicillin resistant Staphylococcus aureus strains in a Spanish hospital: is livestock-contact a risk factor in infections caused by MRSA CC398? International Journal of Medical Microbiology : IJMM, 304(8), 1226-32. https://doi.org/10.1016/j.ijmm.2014.09.004
Benito D, et al. Characterization of Tetracycline and Methicillin Resistant Staphylococcus Aureus Strains in a Spanish Hospital: Is Livestock-contact a Risk Factor in Infections Caused By MRSA CC398. Int J Med Microbiol. 2014;304(8):1226-32. PubMed PMID: 25444568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of tetracycline and methicillin resistant Staphylococcus aureus strains in a Spanish hospital: is livestock-contact a risk factor in infections caused by MRSA CC398? AU - Benito,Daniel, AU - Lozano,Carmen, AU - Rezusta,Antonio, AU - Ferrer,Isabel, AU - Vasquez,Maria Alejandra, AU - Ceballos,Sara, AU - Zarazaga,Myriam, AU - Revillo,Maria José, AU - Torres,Carmen, Y1 - 2014/09/26/ PY - 2014/05/21/received PY - 2014/08/30/revised PY - 2014/09/21/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/7/17/medline KW - Animal contact KW - CC1 KW - CC398 KW - Livestock associated MRSA KW - Multi-resistance KW - Tetracycline SP - 1226 EP - 32 JF - International journal of medical microbiology : IJMM JO - Int J Med Microbiol VL - 304 IS - 8 N2 - UNLABELLED: Tetracycline-resistance (Tet(R)) has been postulated as a marker of the livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) lineage CC398. OBJECTIVES OF THE STUDY: to determine the spa-types and assigned MLST clonal complexes (CCs) among all 98 MRSA-Tet(R) strains recovered during 2011-2012 (from different patients) in a Spanish Hospital, analyzing the possible correlation with livestock-contact of the patients. All 98 strains were assigned to 9 CCs: CC398 (60.2%), CC1 (19.4%), CC5 (12.2%), and other CCs (8.2%). The 98 patients were classified into three groups: (A) contact with livestock-animals (n=25); (B) no-contact with livestock-animals (n=42); (C) no information about animal contact (n=31). A significant higher percentage of CC398 strains was obtained in group A (76%) than in group B (50%) (p<0.05), being the percentage in group C of 61.3%. Most of MRSA-Tet(R)-CC398 strains presented a multi-resistance phenotype, including erythromycin, clindamycin, and ciprofloxacin, and the most prevalent detected genes were tet(M) and erm(C). Three strains presented the phenotype macrolide-susceptibility/lincosamide-resistance and contained the vga(A) gene. MRSA-CC1 strains showed higher percentages of erythromycin/clindamycin resistance (95%/89%) than MRSA-CC398 strains (58%/63%), and this resistance was usually mediated by erm(C) gene. Most of MRSA-CC5 strains showed resistance to ciprofloxacin, tobramycin/kanamycin and erythromycin. None of the strains presented the genes lukF/lukS-PV, tsst-1, eta, etb or etd. All MRSA-CC398 strains lacked the genes of the immune-evasion-cluster, but MRSA-CC1 strains carried these genes (type E). In conclusion, although MRSA CC398 is detected in a significant higher proportion in patients with livestock-contact; its detection in people without this type of contact also indicates its capacity for human-to-human transmission. SN - 1618-0607 UR - https://www.unboundmedicine.com/medline/citation/25444568/Characterization_of_tetracycline_and_methicillin_resistant_Staphylococcus_aureus_strains_in_a_Spanish_hospital:_is_livestock_contact_a_risk_factor_in_infections_caused_by_MRSA_CC398 L2 - https://linkinghub.elsevier.com/retrieve/pii/S1438-4221(14)00120-9 DB - PRIME DP - Unbound Medicine ER -