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Mutations in and Expression of the Tumor Suppressor Gene p53 in Egg-Type Chickens Infected With Subgroup J Avian Leukosis Virus.
Vet Pathol. 2015 Nov; 52(6):1052-6.VP

Abstract

To investigate the molecular mechanisms of the oncogenic effects of avian leukosis virus subgroup J (ALV-J), we examined mutations in and the expression of p53 in the myelocytomas distributed in the liver, spleen, trachea, and bone marrow, as well as in fibrosarcomas in the abdominal cavity and hemangiomas in skin from chickens that were naturally or experimentally infected with ALV-J. Two types of mutations in the p53 gene were detected in myelocytomas of both the experimentally infected and the naturally infected chickens and included point mutations and deletions. Two of the point mutations have not been reported previously. Partial complementary DNA clones with a 122-bp deletion in the p53 gene ORF and a 15-bp deletion in the C-terminus were identified in the myelocytomas. In addition, moderate expression of the mutant p53 protein was detected in the myelocytomas that were distributed in the liver, trachea, spleen, and bone marrow. Mutant p53 protein was not detected in the subcutaneous hemangiomas or in the abdominal fibrosarcomas associated with natural and experimental ALV-J infection, respectively. These results identify mutations associated with abnormal expression of p53 in ALV-J-associated myelocytomas, suggesting a role in tumorigenesis.

Authors+Show Affiliations

Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China College of Animal Husbandry and Veterinary, Liao Ning Medical University, P.R. China College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, P.R. China Liaocheng Vocational and Technical College, Liaocheng, P.R. China.Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China.Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China.Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China.Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, P.R. China.School of Agriculture, Liaocheng University, Liaocheng, P.R. China.College of Animal Husbandry and Veterinary, Liao Ning Medical University, P.R. China.College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, P.R. China liusid@sdau.edu.cn.Division of Avian infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009 xmw@hvri.ac.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25445321

Citation

Yue, Q, et al. "Mutations in and Expression of the Tumor Suppressor Gene P53 in Egg-Type Chickens Infected With Subgroup J Avian Leukosis Virus." Veterinary Pathology, vol. 52, no. 6, 2015, pp. 1052-6.
Yue Q, Yulong G, Liting Q, et al. Mutations in and Expression of the Tumor Suppressor Gene p53 in Egg-Type Chickens Infected With Subgroup J Avian Leukosis Virus. Vet Pathol. 2015;52(6):1052-6.
Yue, Q., Yulong, G., Liting, Q., Shuai, Y., Delong, L., Yubao, L., Lili, J., Sidang, L., & Xiaomei, W. (2015). Mutations in and Expression of the Tumor Suppressor Gene p53 in Egg-Type Chickens Infected With Subgroup J Avian Leukosis Virus. Veterinary Pathology, 52(6), 1052-6. https://doi.org/10.1177/0300985814560232
Yue Q, et al. Mutations in and Expression of the Tumor Suppressor Gene P53 in Egg-Type Chickens Infected With Subgroup J Avian Leukosis Virus. Vet Pathol. 2015;52(6):1052-6. PubMed PMID: 25445321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutations in and Expression of the Tumor Suppressor Gene p53 in Egg-Type Chickens Infected With Subgroup J Avian Leukosis Virus. AU - Yue,Q, AU - Yulong,G, AU - Liting,Q, AU - Shuai,Y, AU - Delong,L, AU - Yubao,L, AU - Lili,J, AU - Sidang,L, AU - Xiaomei,W, Y1 - 2014/12/01/ PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2016/12/15/medline KW - egg-type chicken KW - mutation KW - retroviruses KW - subgroup J avian leukosis KW - tumor suppressor gene p53 SP - 1052 EP - 6 JF - Veterinary pathology JO - Vet Pathol VL - 52 IS - 6 N2 - To investigate the molecular mechanisms of the oncogenic effects of avian leukosis virus subgroup J (ALV-J), we examined mutations in and the expression of p53 in the myelocytomas distributed in the liver, spleen, trachea, and bone marrow, as well as in fibrosarcomas in the abdominal cavity and hemangiomas in skin from chickens that were naturally or experimentally infected with ALV-J. Two types of mutations in the p53 gene were detected in myelocytomas of both the experimentally infected and the naturally infected chickens and included point mutations and deletions. Two of the point mutations have not been reported previously. Partial complementary DNA clones with a 122-bp deletion in the p53 gene ORF and a 15-bp deletion in the C-terminus were identified in the myelocytomas. In addition, moderate expression of the mutant p53 protein was detected in the myelocytomas that were distributed in the liver, trachea, spleen, and bone marrow. Mutant p53 protein was not detected in the subcutaneous hemangiomas or in the abdominal fibrosarcomas associated with natural and experimental ALV-J infection, respectively. These results identify mutations associated with abnormal expression of p53 in ALV-J-associated myelocytomas, suggesting a role in tumorigenesis. SN - 1544-2217 UR - https://www.unboundmedicine.com/medline/citation/25445321/Mutations_in_and_Expression_of_the_Tumor_Suppressor_Gene_p53_in_Egg_Type_Chickens_Infected_With_Subgroup_J_Avian_Leukosis_Virus_ L2 - https://journals.sagepub.com/doi/10.1177/0300985814560232?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -