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Identification, phylogenetic evolutionary analysis of GDQY orf virus isolated from Qingyuan City, Guangdong Province, southern China.
Gene 2015; 555(2):260-8GENE

Abstract

Infection with the orf virus (ORFV) leads to contagious ecthyma, also called contagious pustular dermatitis, which usually affects sheep, goats and other small ruminants. It has a great distribution throughout the world and has also been reported to infect humans. Though many strains have been isolated from differing parts of mainland China, rarely has any strain been reported from the southern provinces of China. We studied a case of orf virus infection that occurred at Qingyuan City, Guangdong Province in southern China. An orf virus strain, GDQY, was successfully isolated and identified through cell culture techniques and transmission electron microscopy. Complete genes of ORFV011, ORFV059, ORFV106 and ORFV107 were amplified for the sequence analysis based on their nucleotide or amino acid level. In order to discuss the genetic variation, precise sequences were used to compare to other reference strains isolated from different districts or countries. Phylogenetic trees based on those strains were built up and evolutionary distances were calculated based on the alignment of their complete sequences. The typical structure of the orf virus was observed in cell-culture suspensions inoculated with GDQY, and the full-length of four genes was amplified and sequenced. Phylogenetic analysis indicated that GDQY is homologous to FJ-DS and CQ/WZ on ORFV011 nucleotides. ORFV059 may be more variable than ORFV011 based on the comparison between GDQY and other isolates. Genetic studies of ORFV106 and 107 are reported for the first time in the presented study.

Authors+Show Affiliations

Laboratory of Clinical, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China. Electronic address: 1725012289@qq.com.Laboratory of Clinical, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China.Laboratory of Clinical, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China.Laboratory of Clinical, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China.Laboratory of Clinical, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China; Medical Research Center, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China.Laboratory of Clinical, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, The Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China.School of Biotechnology, Southern Medical University, Guangzhou 510515, People's Republic of China.School of Biotechnology, Southern Medical University, Guangzhou 510515, People's Republic of China.School of Biotechnology, Southern Medical University, Guangzhou 510515, People's Republic of China. Electronic address: shluo815@yahoo.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25447901

Citation

Duan, Chaohui, et al. "Identification, Phylogenetic Evolutionary Analysis of GDQY Orf Virus Isolated From Qingyuan City, Guangdong Province, Southern China." Gene, vol. 555, no. 2, 2015, pp. 260-8.
Duan C, Liao M, Wang H, et al. Identification, phylogenetic evolutionary analysis of GDQY orf virus isolated from Qingyuan City, Guangdong Province, southern China. Gene. 2015;555(2):260-8.
Duan, C., Liao, M., Wang, H., Luo, X., Shao, J., Xu, Y., ... Luo, S. (2015). Identification, phylogenetic evolutionary analysis of GDQY orf virus isolated from Qingyuan City, Guangdong Province, southern China. Gene, 555(2), pp. 260-8. doi:10.1016/j.gene.2014.11.016.
Duan C, et al. Identification, Phylogenetic Evolutionary Analysis of GDQY Orf Virus Isolated From Qingyuan City, Guangdong Province, Southern China. Gene. 2015 Jan 25;555(2):260-8. PubMed PMID: 25447901.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification, phylogenetic evolutionary analysis of GDQY orf virus isolated from Qingyuan City, Guangdong Province, southern China. AU - Duan,Chaohui, AU - Liao,Meiying, AU - Wang,Han, AU - Luo,Xiaohong, AU - Shao,Jing, AU - Xu,Ying, AU - Li,Wei, AU - Hao,Wenbo, AU - Luo,Shuhong, Y1 - 2014/11/12/ PY - 2014/09/18/received PY - 2014/11/09/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/2/18/medline KW - Genetic diversity KW - Goat KW - Orf virus KW - Phylogenetic analysis KW - Southern China SP - 260 EP - 8 JF - Gene JO - Gene VL - 555 IS - 2 N2 - Infection with the orf virus (ORFV) leads to contagious ecthyma, also called contagious pustular dermatitis, which usually affects sheep, goats and other small ruminants. It has a great distribution throughout the world and has also been reported to infect humans. Though many strains have been isolated from differing parts of mainland China, rarely has any strain been reported from the southern provinces of China. We studied a case of orf virus infection that occurred at Qingyuan City, Guangdong Province in southern China. An orf virus strain, GDQY, was successfully isolated and identified through cell culture techniques and transmission electron microscopy. Complete genes of ORFV011, ORFV059, ORFV106 and ORFV107 were amplified for the sequence analysis based on their nucleotide or amino acid level. In order to discuss the genetic variation, precise sequences were used to compare to other reference strains isolated from different districts or countries. Phylogenetic trees based on those strains were built up and evolutionary distances were calculated based on the alignment of their complete sequences. The typical structure of the orf virus was observed in cell-culture suspensions inoculated with GDQY, and the full-length of four genes was amplified and sequenced. Phylogenetic analysis indicated that GDQY is homologous to FJ-DS and CQ/WZ on ORFV011 nucleotides. ORFV059 may be more variable than ORFV011 based on the comparison between GDQY and other isolates. Genetic studies of ORFV106 and 107 are reported for the first time in the presented study. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/25447901/Identification_phylogenetic_evolutionary_analysis_of_GDQY_orf_virus_isolated_from_Qingyuan_City_Guangdong_Province_southern_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(14)01277-3 DB - PRIME DP - Unbound Medicine ER -