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Comparison of in vitro toxicity of silver ions and silver nanoparticles on human hepatoma cells.
Environ Toxicol. 2016 Jun; 31(6):679-92.ET

Abstract

Scientific information on the potential harmful effects of silver nanoparticles (AgNPs) on human health severely lags behind their exponentially growing applications in consumer products. In assessing the toxic risk of AgNP usage, liver, as a detoxifying organ, is particularly important. The aim of this study was to explore the toxicity mechanisms of nano and ionic forms of silver on human hepatoblastoma (HepG2) cells. The results showed that silver ions and citrate-coated AgNPs reduced cell viability in a dose-dependent manner. The IC50 values of silver ions and citrate-coated AgNPs were 0.5 and 50 mg L(-1) , respectively. The LDH leakage and inhibition of albumin synthesis, along with decreased ALT activity, indicated that treatment with either AgNP or Ag ions resulted in membrane damage and reduced the cell function of human liver cells. Evaluation of oxidative stress markers demonstrating depletion of GSH, increased ROS production, and increased SOD activity, indicated that oxidative stress might contribute to the toxicity effects of nano and ionic forms of silver. The observed toxic effect of AgNP on HepG2 cells was substantially weaker than that caused by ionic silver, while the uptake of nano and ionic forms of silver by HepG2 cells was nearly the same. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 679-692, 2016.

Authors+Show Affiliations

Institute for Medical Research and Occupational Health, Analytical Toxicology and Mineral Metabolism Unit, Ksaverska cesta 2, 10 000, Zagreb, Croatia.University of Zagreb, Faculty of Pharmacy and Biochemistry, Department for Clinical Chemistry, Ante Kovačića 1, 10 000, Zagreb, Croatia.University of Zagreb, Faculty of Pharmacy and Biochemistry, Department for Clinical Chemistry, Ante Kovačića 1, 10 000, Zagreb, Croatia.Institute for Medical Research and Occupational Health, Analytical Toxicology and Mineral Metabolism Unit, Ksaverska cesta 2, 10 000, Zagreb, Croatia.Ruđer Bošković Institute, Division of Physical Chemistry, Bijenička cesta 54, 10 000, Zagreb, Croatia.University of Zagreb, School of Medicine, Department for Hystology and Embryology, Šalata 3, 10 000, Zagreb, Croatia.Institute for Chemistry, Analytical Chemistry, Karl-Franzens University, Universitätsplatz 1, 8010, Graz, Austria.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

25448069

Citation

Vrček, Ivana Vinković, et al. "Comparison of in Vitro Toxicity of Silver Ions and Silver Nanoparticles On Human Hepatoma Cells." Environmental Toxicology, vol. 31, no. 6, 2016, pp. 679-92.
Vrček IV, Žuntar I, Petlevski R, et al. Comparison of in vitro toxicity of silver ions and silver nanoparticles on human hepatoma cells. Environ Toxicol. 2016;31(6):679-92.
Vrček, I. V., Žuntar, I., Petlevski, R., Pavičić, I., Dutour Sikirić, M., Ćurlin, M., & Goessler, W. (2016). Comparison of in vitro toxicity of silver ions and silver nanoparticles on human hepatoma cells. Environmental Toxicology, 31(6), 679-92. https://doi.org/10.1002/tox.22081
Vrček IV, et al. Comparison of in Vitro Toxicity of Silver Ions and Silver Nanoparticles On Human Hepatoma Cells. Environ Toxicol. 2016;31(6):679-92. PubMed PMID: 25448069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of in vitro toxicity of silver ions and silver nanoparticles on human hepatoma cells. AU - Vrček,Ivana Vinković, AU - Žuntar,Irena, AU - Petlevski,Roberta, AU - Pavičić,Ivan, AU - Dutour Sikirić,Maja, AU - Ćurlin,Marija, AU - Goessler,Walter, Y1 - 2014/11/28/ PY - 2014/02/02/received PY - 2014/11/05/revised PY - 2014/11/09/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2017/3/3/medline KW - HepG2 cells KW - cytotoxicity KW - ionic silver KW - oxidative stress KW - silver nanoparticles SP - 679 EP - 92 JF - Environmental toxicology JO - Environ Toxicol VL - 31 IS - 6 N2 - Scientific information on the potential harmful effects of silver nanoparticles (AgNPs) on human health severely lags behind their exponentially growing applications in consumer products. In assessing the toxic risk of AgNP usage, liver, as a detoxifying organ, is particularly important. The aim of this study was to explore the toxicity mechanisms of nano and ionic forms of silver on human hepatoblastoma (HepG2) cells. The results showed that silver ions and citrate-coated AgNPs reduced cell viability in a dose-dependent manner. The IC50 values of silver ions and citrate-coated AgNPs were 0.5 and 50 mg L(-1) , respectively. The LDH leakage and inhibition of albumin synthesis, along with decreased ALT activity, indicated that treatment with either AgNP or Ag ions resulted in membrane damage and reduced the cell function of human liver cells. Evaluation of oxidative stress markers demonstrating depletion of GSH, increased ROS production, and increased SOD activity, indicated that oxidative stress might contribute to the toxicity effects of nano and ionic forms of silver. The observed toxic effect of AgNP on HepG2 cells was substantially weaker than that caused by ionic silver, while the uptake of nano and ionic forms of silver by HepG2 cells was nearly the same. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 679-692, 2016. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/25448069/Comparison_of_in_vitro_toxicity_of_silver_ions_and_silver_nanoparticles_on_human_hepatoma_cells_ L2 - https://doi.org/10.1002/tox.22081 DB - PRIME DP - Unbound Medicine ER -