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The effects of combined dietary probiotics Lactococcus lactis BFE920 and Lactobacillus plantarum FGL0001 on innate immunity and disease resistance in olive flounder (Paralichthys olivaceus).
Fish Shellfish Immunol. 2015 Jan; 42(1):177-83.FS

Abstract

The effects of a dietary probiotic mixture containing Lactococcus (Lc.) lactis BFE920 isolated from bean sprout and autochthonous Lactobacillus (Lb.) plantarum FGL0001 originally isolated from the hindgut of olive flounder (Paralichthys olivaceus) were investigated for the purpose of improving the probiotic effects of Lc. lactis BFE920 on the olive flounder. The immunostimulatory, disease protective, and weight gain effects of Lc. lactis BFE920 were significantly improved when olive flounder (average weight 37.5±1.26 g) were fed the probiotic mixture (log10 7.0 CFU each/g feed pellet) for 30 days. Flounder fed the mixture showed improved skin mucus lysozyme activity and phagocytic activity of innate immune cells compared to flounder fed a single probiotic agent or a control diet. While the levels of neutrophil activity in flounder fed the single probiotic agent or the mixture were similar, they were significantly higher than levels in a control group. Additionally, probiotic-fed flounder showed significantly increased expressions of IL-6, IL-8, and TNF-α in the intestine compared to the control group. Following a 30-day period of being fed probiotics or a control diet, the olive flounder were challenged with an i.p. injection of Streptococcus iniae (log10 6.0 CFU/fish). The groups fed the mixed probiotics, Lc. lactis BFE920, Lb. plantarum FGL0001, and the control diet had survival rates of 55%, 45%, 35%, and 20%, respectively. Flounder fed the probiotic mixture gained 38.1±2.8% more body weight compared to flounder fed the control diet during the 30-day study period. These data strongly suggest that a mixture of Lc. lactis BFE920 and Lb. plantarum FGL0001 may serve as an immunostimulating feed additive useful for disease protection in the fish farming industry.

Authors+Show Affiliations

School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.GyeongSangbuk-Do Fisheries Technology Center, Pohang 791-941, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.Pathology Division, National Fisheries Research and Development Institute, Busan 619-902, Republic of Korea.Pathology Division, National Fisheries Research and Development Institute, Busan 619-902, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea. Electronic address: sksong@handong.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25449382

Citation

Beck, Bo Ram, et al. "The Effects of Combined Dietary Probiotics Lactococcus Lactis BFE920 and Lactobacillus Plantarum FGL0001 On Innate Immunity and Disease Resistance in Olive Flounder (Paralichthys Olivaceus)." Fish & Shellfish Immunology, vol. 42, no. 1, 2015, pp. 177-83.
Beck BR, Kim D, Jeon J, et al. The effects of combined dietary probiotics Lactococcus lactis BFE920 and Lactobacillus plantarum FGL0001 on innate immunity and disease resistance in olive flounder (Paralichthys olivaceus). Fish Shellfish Immunol. 2015;42(1):177-83.
Beck, B. R., Kim, D., Jeon, J., Lee, S. M., Kim, H. K., Kim, O. J., Lee, J. I., Suh, B. S., Do, H. K., Lee, K. H., Holzapfel, W. H., Hwang, J. Y., Kwon, M. G., & Song, S. K. (2015). The effects of combined dietary probiotics Lactococcus lactis BFE920 and Lactobacillus plantarum FGL0001 on innate immunity and disease resistance in olive flounder (Paralichthys olivaceus). Fish & Shellfish Immunology, 42(1), 177-83. https://doi.org/10.1016/j.fsi.2014.10.035
Beck BR, et al. The Effects of Combined Dietary Probiotics Lactococcus Lactis BFE920 and Lactobacillus Plantarum FGL0001 On Innate Immunity and Disease Resistance in Olive Flounder (Paralichthys Olivaceus). Fish Shellfish Immunol. 2015;42(1):177-83. PubMed PMID: 25449382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of combined dietary probiotics Lactococcus lactis BFE920 and Lactobacillus plantarum FGL0001 on innate immunity and disease resistance in olive flounder (Paralichthys olivaceus). AU - Beck,Bo Ram, AU - Kim,Daniel, AU - Jeon,Jongsu, AU - Lee,Sun-Min, AU - Kim,Hui Kwon, AU - Kim,Oi-Jin, AU - Lee,Jae Il, AU - Suh,Byung Sun, AU - Do,Hyung Ki, AU - Lee,Kwan Hee, AU - Holzapfel,Wilhelm H, AU - Hwang,Jee Youn, AU - Kwon,Mun Gyeong, AU - Song,Seong Kyu, Y1 - 2014/11/07/ PY - 2014/07/17/received PY - 2014/10/20/revised PY - 2014/10/28/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/8/12/medline KW - Innate immunity KW - Lactobacillus plantarum FGL0001 KW - Lactococcus lactis BFE920 KW - Olive flounder KW - Probiotics SP - 177 EP - 83 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 42 IS - 1 N2 - The effects of a dietary probiotic mixture containing Lactococcus (Lc.) lactis BFE920 isolated from bean sprout and autochthonous Lactobacillus (Lb.) plantarum FGL0001 originally isolated from the hindgut of olive flounder (Paralichthys olivaceus) were investigated for the purpose of improving the probiotic effects of Lc. lactis BFE920 on the olive flounder. The immunostimulatory, disease protective, and weight gain effects of Lc. lactis BFE920 were significantly improved when olive flounder (average weight 37.5±1.26 g) were fed the probiotic mixture (log10 7.0 CFU each/g feed pellet) for 30 days. Flounder fed the mixture showed improved skin mucus lysozyme activity and phagocytic activity of innate immune cells compared to flounder fed a single probiotic agent or a control diet. While the levels of neutrophil activity in flounder fed the single probiotic agent or the mixture were similar, they were significantly higher than levels in a control group. Additionally, probiotic-fed flounder showed significantly increased expressions of IL-6, IL-8, and TNF-α in the intestine compared to the control group. Following a 30-day period of being fed probiotics or a control diet, the olive flounder were challenged with an i.p. injection of Streptococcus iniae (log10 6.0 CFU/fish). The groups fed the mixed probiotics, Lc. lactis BFE920, Lb. plantarum FGL0001, and the control diet had survival rates of 55%, 45%, 35%, and 20%, respectively. Flounder fed the probiotic mixture gained 38.1±2.8% more body weight compared to flounder fed the control diet during the 30-day study period. These data strongly suggest that a mixture of Lc. lactis BFE920 and Lb. plantarum FGL0001 may serve as an immunostimulating feed additive useful for disease protection in the fish farming industry. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/25449382/The_effects_of_combined_dietary_probiotics_Lactococcus_lactis_BFE920_and_Lactobacillus_plantarum_FGL0001_on_innate_immunity_and_disease_resistance_in_olive_flounder__Paralichthys_olivaceus__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(14)00409-4 DB - PRIME DP - Unbound Medicine ER -