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The AQP-3 water channel and the ClC-3 chloride channel coordinate the hypotonicity-induced swelling volume in nasopharyngeal carcinoma cells.
Int J Biochem Cell Biol 2014; 57:96-107IJ

Abstract

Cell volume regulation is a fundamental activity to maintain cell survival, and aquaporins and chloride channels play important roles in this process. However, the interactions between these channels are far from clear. In this study, the interactions between AQP-3 and ClC-3 were investigated in CNE-1 and CNE-2Z nasopharyngeal carcinoma cells, which are well and poorly differentiated, respectively. The correlation coefficient of AQP-3 and ClC-3 protein phylogenetic trees was 0.319. In CNE-1 cells, there are overlapping distributions of AQP-3 and ClC-3, mainly in the plasma membrane. This was confirmed by the co-immunoprecipitation of AQP-3 and ClC-3, showing that they could be interlinked and form complexes. AQP-3 over-expression had no significant effects on swelling-induced Cl(-) currents (ICl,swell); however, ICl,swell could be inhibited by aquaporin blockers, anti-AQP-3 antibodies and AQP-3-siRNAs. In addition, the AQP-3 expression was decreased by down-regulation of ClC-3 expression, indicating that ClC-3 can modulate the expression of AQP-3 proteins. The effects of aquaporin blockers, anti-AQP-3 antibodies and AQP-3 over-expression on ICl,swell in CNE-2Z cells were consistent with those in CNE-1 cells. In conclusion, AQP-3 and ClC-3 are functionally-related integral membrane channel proteins, and their interactions are involved in cell volume regulation in CNE-1 and CNE-2Z cells. The opening of ClC-3 transports Cl(-) across the cell membrane and then drives the efflux of water through AQP-3 channels and ion channels; AQP-3 may interact with ClC-3 in order to regulate the effluxes of chloride and water.

Authors+Show Affiliations

Department of Pathology, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: zhf19841011@126.com.Department of Physiology, Medical College, Jinan University, Guangzhou 510632, China.Research Institute of Atherosclerotic Disease, and Laboratory Animal Center, Xi'an Jiaotong University Cardiovascular Research Center, Xi'an 710061, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China.Guangdong Key Laboratory for Bioactive Drugs Research and Department of Biology, Guangdong Pharmaceutical University, Guangzhou 510006, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou 510632, China. Electronic address: chenlixinw@sohu.com.Department of Physiology, Medical College, Jinan University, Guangzhou 510632, China. Electronic address: twangliwei@jnu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25450461

Citation

Zhang, Haifeng, et al. "The AQP-3 Water Channel and the ClC-3 Chloride Channel Coordinate the Hypotonicity-induced Swelling Volume in Nasopharyngeal Carcinoma Cells." The International Journal of Biochemistry & Cell Biology, vol. 57, 2014, pp. 96-107.
Zhang H, Li H, Liu E, et al. The AQP-3 water channel and the ClC-3 chloride channel coordinate the hypotonicity-induced swelling volume in nasopharyngeal carcinoma cells. Int J Biochem Cell Biol. 2014;57:96-107.
Zhang, H., Li, H., Liu, E., Guang, Y., Yang, L., Mao, J., ... Wang, L. (2014). The AQP-3 water channel and the ClC-3 chloride channel coordinate the hypotonicity-induced swelling volume in nasopharyngeal carcinoma cells. The International Journal of Biochemistry & Cell Biology, 57, pp. 96-107. doi:10.1016/j.biocel.2014.10.014.
Zhang H, et al. The AQP-3 Water Channel and the ClC-3 Chloride Channel Coordinate the Hypotonicity-induced Swelling Volume in Nasopharyngeal Carcinoma Cells. Int J Biochem Cell Biol. 2014;57:96-107. PubMed PMID: 25450461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The AQP-3 water channel and the ClC-3 chloride channel coordinate the hypotonicity-induced swelling volume in nasopharyngeal carcinoma cells. AU - Zhang,Haifeng, AU - Li,Huarong, AU - Liu,Enqi, AU - Guang,Yutao, AU - Yang,Lili, AU - Mao,Jianwen, AU - Zhu,Linyan, AU - Chen,Lixin, AU - Wang,Liwei, Y1 - 2014/10/19/ PY - 2014/04/09/received PY - 2014/10/06/revised PY - 2014/10/08/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/9/16/medline KW - AQP-3 KW - Aquaporins KW - Cell volume regulation KW - Chloride channels KW - ClC-3 SP - 96 EP - 107 JF - The international journal of biochemistry & cell biology JO - Int. J. Biochem. Cell Biol. VL - 57 N2 - Cell volume regulation is a fundamental activity to maintain cell survival, and aquaporins and chloride channels play important roles in this process. However, the interactions between these channels are far from clear. In this study, the interactions between AQP-3 and ClC-3 were investigated in CNE-1 and CNE-2Z nasopharyngeal carcinoma cells, which are well and poorly differentiated, respectively. The correlation coefficient of AQP-3 and ClC-3 protein phylogenetic trees was 0.319. In CNE-1 cells, there are overlapping distributions of AQP-3 and ClC-3, mainly in the plasma membrane. This was confirmed by the co-immunoprecipitation of AQP-3 and ClC-3, showing that they could be interlinked and form complexes. AQP-3 over-expression had no significant effects on swelling-induced Cl(-) currents (ICl,swell); however, ICl,swell could be inhibited by aquaporin blockers, anti-AQP-3 antibodies and AQP-3-siRNAs. In addition, the AQP-3 expression was decreased by down-regulation of ClC-3 expression, indicating that ClC-3 can modulate the expression of AQP-3 proteins. The effects of aquaporin blockers, anti-AQP-3 antibodies and AQP-3 over-expression on ICl,swell in CNE-2Z cells were consistent with those in CNE-1 cells. In conclusion, AQP-3 and ClC-3 are functionally-related integral membrane channel proteins, and their interactions are involved in cell volume regulation in CNE-1 and CNE-2Z cells. The opening of ClC-3 transports Cl(-) across the cell membrane and then drives the efflux of water through AQP-3 channels and ion channels; AQP-3 may interact with ClC-3 in order to regulate the effluxes of chloride and water. SN - 1878-5875 UR - https://www.unboundmedicine.com/medline/citation/25450461/The_AQP_3_water_channel_and_the_ClC_3_chloride_channel_coordinate_the_hypotonicity_induced_swelling_volume_in_nasopharyngeal_carcinoma_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1357-2725(14)00325-2 DB - PRIME DP - Unbound Medicine ER -