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Transient receptor potential vanilloid 1 is expressed in human cytotrophoblasts: induction of cell apoptosis and impairment of syncytialization.
Int J Biochem Cell Biol. 2014 Dec; 57:177-85.IJ

Abstract

The normal development of placenta relies essentially on a balanced proliferation, differentiation and apoptosis of cytotrophoblasts. These processes are tightly regulated by several hormones, cytokines, lipids and other molecules and anomalies in these events are associated with gestational complications. The cation channel transient receptor potential vanilloid 1 (TRPV1) is expressed in several organs and tissues and it participates in cellular events like nociception, inflammation and cell death. However, the expression and importance of this receptor in human placenta still remains unknown. In this work, we found that TRPV1 is expressed in human cytotrophoblasts and syncytiotrophoblasts. Furthermore, the TRPV1 agonists capsaicin and anandamide decreased cytotrophoblast viability and induced morphological alterations, such as chromatin condensation and fragmentation, which suggest the occurrence of apoptosis. Also, both TRPV1 agonists induced a loss of mitochondrial membrane potential and an increase of caspase 3/7 activity and production of reactive species of oxygen and nitrogen. Furthermore, capsaicin (10 μM) impaired the spontaneous in vitro differentiation of cytotrophoblasts into syncytiotrophoblasts by triggering TRPV1, as observed by the decrease in placental alkaline phosphatase activity and in human chorionic gonadotropin secretion. On the other hand, anandamide decreased placental alkaline phosphatase activity via a TRPV1-independent mechanism but did not influence the secretion of human chorionic gonadotropin. In conclusion, we showed that TRPV1 is expressed in human cytotrophoblasts and syncytiotrophoblasts and also reported the involvement of this receptor in cytotrophoblast apoptosis and differentiation.

Authors+Show Affiliations

Departamento de Ciências Biológicas, Laboratório de Bioquímica, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal; Instituto de Biologia Molecular e Celular da Universidade do Porto (IBMC), Porto, Portugal.Departamento de Ciências Biológicas, Laboratório de Bioquímica, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal; Instituto de Biologia Molecular e Celular da Universidade do Porto (IBMC), Porto, Portugal.Departamento de Bioquímica (U38-FCT), Faculdade de Medicina, Universidade do Porto, Porto, Portugal; Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal.Departamento de Ciências Biológicas, Laboratório de Bioquímica, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal; Instituto de Biologia Molecular e Celular da Universidade do Porto (IBMC), Porto, Portugal.Departamento de Ciências Biológicas, Laboratório de Bioquímica, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal; Instituto de Biologia Molecular e Celular da Universidade do Porto (IBMC), Porto, Portugal. Electronic address: george@ff.up.pt.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25450464

Citation

Costa, M A., et al. "Transient Receptor Potential Vanilloid 1 Is Expressed in Human Cytotrophoblasts: Induction of Cell Apoptosis and Impairment of Syncytialization." The International Journal of Biochemistry & Cell Biology, vol. 57, 2014, pp. 177-85.
Costa MA, Fonseca BM, Keating E, et al. Transient receptor potential vanilloid 1 is expressed in human cytotrophoblasts: induction of cell apoptosis and impairment of syncytialization. Int J Biochem Cell Biol. 2014;57:177-85.
Costa, M. A., Fonseca, B. M., Keating, E., Teixeira, N. A., & Correia-da-Silva, G. (2014). Transient receptor potential vanilloid 1 is expressed in human cytotrophoblasts: induction of cell apoptosis and impairment of syncytialization. The International Journal of Biochemistry & Cell Biology, 57, 177-85. https://doi.org/10.1016/j.biocel.2014.10.008
Costa MA, et al. Transient Receptor Potential Vanilloid 1 Is Expressed in Human Cytotrophoblasts: Induction of Cell Apoptosis and Impairment of Syncytialization. Int J Biochem Cell Biol. 2014;57:177-85. PubMed PMID: 25450464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transient receptor potential vanilloid 1 is expressed in human cytotrophoblasts: induction of cell apoptosis and impairment of syncytialization. AU - Costa,M A, AU - Fonseca,B M, AU - Keating,E, AU - Teixeira,N A, AU - Correia-da-Silva,G, Y1 - 2014/10/14/ PY - 2014/05/28/received PY - 2014/09/25/revised PY - 2014/10/06/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/9/16/medline KW - Anandamide KW - Apoptosis KW - Capsaicin KW - Cytotrophoblasts KW - Syncytialization KW - TRPV1 SP - 177 EP - 85 JF - The international journal of biochemistry & cell biology JO - Int J Biochem Cell Biol VL - 57 N2 - The normal development of placenta relies essentially on a balanced proliferation, differentiation and apoptosis of cytotrophoblasts. These processes are tightly regulated by several hormones, cytokines, lipids and other molecules and anomalies in these events are associated with gestational complications. The cation channel transient receptor potential vanilloid 1 (TRPV1) is expressed in several organs and tissues and it participates in cellular events like nociception, inflammation and cell death. However, the expression and importance of this receptor in human placenta still remains unknown. In this work, we found that TRPV1 is expressed in human cytotrophoblasts and syncytiotrophoblasts. Furthermore, the TRPV1 agonists capsaicin and anandamide decreased cytotrophoblast viability and induced morphological alterations, such as chromatin condensation and fragmentation, which suggest the occurrence of apoptosis. Also, both TRPV1 agonists induced a loss of mitochondrial membrane potential and an increase of caspase 3/7 activity and production of reactive species of oxygen and nitrogen. Furthermore, capsaicin (10 μM) impaired the spontaneous in vitro differentiation of cytotrophoblasts into syncytiotrophoblasts by triggering TRPV1, as observed by the decrease in placental alkaline phosphatase activity and in human chorionic gonadotropin secretion. On the other hand, anandamide decreased placental alkaline phosphatase activity via a TRPV1-independent mechanism but did not influence the secretion of human chorionic gonadotropin. In conclusion, we showed that TRPV1 is expressed in human cytotrophoblasts and syncytiotrophoblasts and also reported the involvement of this receptor in cytotrophoblast apoptosis and differentiation. SN - 1878-5875 UR - https://www.unboundmedicine.com/medline/citation/25450464/Transient_receptor_potential_vanilloid_1_is_expressed_in_human_cytotrophoblasts:_induction_of_cell_apoptosis_and_impairment_of_syncytialization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1357-2725(14)00319-7 DB - PRIME DP - Unbound Medicine ER -