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A novel granulocyte-specific α integrin is essential for cellular immunity in the silkworm Bombyx mori.
J Insect Physiol. 2014 Dec; 71:61-7.JI

Abstract

Haemocytes play crucial roles in immune responses and survival in insects. Specific cell markers have proven effective in clarifying the function and haematopoiesis of haemocytes. The silkworm Bombyx mori is a good model for studying insect haemocytes; however, little is known about haemocyte-specific markers or their functions in silkworm. In this study, we identified the α subunit of integrin, BmintegrinαPS3, as being specifically and highly expressed in silkworm haemocytes. Immunofluorescence analysis validated the specificity of BmintegrinαPS3 in larval granulocytes. Further analyses indicated that haemocytes dispersed from haematopoietic organs (HPOs) into the circulating haemolymph could differentiate into granulocytes. In addition, the processes of encapsulation and phagocytosis were controlled by larval granulocytes. Our work demonstrated that BmintegrinαPS3 could be used as a specific marker for granulocytes and could be applied to future molecular cell biology studies.

Authors+Show Affiliations

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. Electronic address: hcui@swu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25450560

Citation

Zhang, Kui, et al. "A Novel Granulocyte-specific Α Integrin Is Essential for Cellular Immunity in the Silkworm Bombyx Mori." Journal of Insect Physiology, vol. 71, 2014, pp. 61-7.
Zhang K, Tan J, Xu M, et al. A novel granulocyte-specific α integrin is essential for cellular immunity in the silkworm Bombyx mori. J Insect Physiol. 2014;71:61-7.
Zhang, K., Tan, J., Xu, M., Su, J., Hu, R., Chen, Y., Xuan, F., Yang, R., & Cui, H. (2014). A novel granulocyte-specific α integrin is essential for cellular immunity in the silkworm Bombyx mori. Journal of Insect Physiology, 71, 61-7. https://doi.org/10.1016/j.jinsphys.2014.10.007
Zhang K, et al. A Novel Granulocyte-specific Α Integrin Is Essential for Cellular Immunity in the Silkworm Bombyx Mori. J Insect Physiol. 2014;71:61-7. PubMed PMID: 25450560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel granulocyte-specific α integrin is essential for cellular immunity in the silkworm Bombyx mori. AU - Zhang,Kui, AU - Tan,Juan, AU - Xu,Man, AU - Su,Jingjing, AU - Hu,Renjian, AU - Chen,Yibiao, AU - Xuan,Fan, AU - Yang,Rui, AU - Cui,Hongjuan, Y1 - 2014/10/18/ PY - 2014/03/18/received PY - 2014/10/10/revised PY - 2014/10/13/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/7/23/medline KW - Bombyx mori KW - Granulocytes KW - Immune responses KW - IntegrinαPS3 KW - Marker SP - 61 EP - 7 JF - Journal of insect physiology JO - J. Insect Physiol. VL - 71 N2 - Haemocytes play crucial roles in immune responses and survival in insects. Specific cell markers have proven effective in clarifying the function and haematopoiesis of haemocytes. The silkworm Bombyx mori is a good model for studying insect haemocytes; however, little is known about haemocyte-specific markers or their functions in silkworm. In this study, we identified the α subunit of integrin, BmintegrinαPS3, as being specifically and highly expressed in silkworm haemocytes. Immunofluorescence analysis validated the specificity of BmintegrinαPS3 in larval granulocytes. Further analyses indicated that haemocytes dispersed from haematopoietic organs (HPOs) into the circulating haemolymph could differentiate into granulocytes. In addition, the processes of encapsulation and phagocytosis were controlled by larval granulocytes. Our work demonstrated that BmintegrinαPS3 could be used as a specific marker for granulocytes and could be applied to future molecular cell biology studies. SN - 1879-1611 UR - https://www.unboundmedicine.com/medline/citation/25450560/A_novel_granulocyte_specific_α_integrin_is_essential_for_cellular_immunity_in_the_silkworm_Bombyx_mori_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-1910(14)00202-9 DB - PRIME DP - Unbound Medicine ER -