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Biochemical responses in the gills of Meretrix meretrix after exposure to treated municipal effluent.
Ecotoxicol Environ Saf. 2015 Jan; 111:78-85.EE

Abstract

The biochemical effects in marine bivalves exposed to increasing concentrations of treated municipal effluent (TME), as discharged into receiving marine waters, are investigated. The effluent was collected from a municipal sewage treatment plant (STP) in Qingdao (China). Meretrix meretrix were exposed to effluent volume ratio (EVR, ratio of effluent volume accounted for tailwater seawater mixture) 0%, 1%, 5%, 10%, and 20% (v/v) TME for 15 days and the following biochemical responses in gills were measured: (1) the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione (GSH) content, and lipid peroxidation levels of malondialdehyde (MDA), as oxidative stress biomarkers; (2) the activity of 7-ethoxyresorufin O-deethylase (EROD) and gluthathione S-transferase (GST), as phase I and phase II conjugation enzymes, respectively; (3) acetylcholinesterase (AChE), as a biomarker of neurotoxicity, and (4) metallothioneins (MTs), as proteins strongly induced by heavy metals. Most of the biochemical indices present high and significant variation frequency (above 50%). There is enhancement in the antioxidant enzymes, EROD, GST, AChE, and MTs, as well as consumption of GSH. The current experimental results suggest that effluent with concentrations less than 20% (v/v) do not cause lipid peroxidation damage. This implies that the activated defense is sufficient to protect the bivalves' gill tissues from cytotoxicity produced by the effluent. Furthermore, GSH, GPx, MTs, and GR are suitable, and sufficiently sensitive, biomarkers to indicate the pollution levels in marine environments receiving such effluent.

Authors+Show Affiliations

Key Laboratory for Marine Environment and Ecology of Ministry of Education, Ocean University of China, Qingdao, Shandong Province 266100, China.Key Laboratory for Marine Environment and Ecology of Ministry of Education, Ocean University of China, Qingdao, Shandong Province 266100, China.Key Laboratory for Marine Environment and Ecology of Ministry of Education, Ocean University of China, Qingdao, Shandong Province 266100, China.Key Laboratory for Marine Environment and Ecology of Ministry of Education, Ocean University of China, Qingdao, Shandong Province 266100, China.Key Laboratory for Marine Environment and Ecology of Ministry of Education, Ocean University of China, Qingdao, Shandong Province 266100, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25450918

Citation

Wan, Ru, et al. "Biochemical Responses in the Gills of Meretrix Meretrix After Exposure to Treated Municipal Effluent." Ecotoxicology and Environmental Safety, vol. 111, 2015, pp. 78-85.
Wan R, Meng F, Fu W, et al. Biochemical responses in the gills of Meretrix meretrix after exposure to treated municipal effluent. Ecotoxicol Environ Saf. 2015;111:78-85.
Wan, R., Meng, F., Fu, W., Wang, Q., & Su, E. (2015). Biochemical responses in the gills of Meretrix meretrix after exposure to treated municipal effluent. Ecotoxicology and Environmental Safety, 111, 78-85. https://doi.org/10.1016/j.ecoenv.2014.09.038
Wan R, et al. Biochemical Responses in the Gills of Meretrix Meretrix After Exposure to Treated Municipal Effluent. Ecotoxicol Environ Saf. 2015;111:78-85. PubMed PMID: 25450918.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biochemical responses in the gills of Meretrix meretrix after exposure to treated municipal effluent. AU - Wan,Ru, AU - Meng,Fanping, AU - Fu,Wenchao, AU - Wang,Qun, AU - Su,Enping, Y1 - 2014/10/17/ PY - 2014/07/23/received PY - 2014/09/24/revised PY - 2014/09/26/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/7/30/medline KW - Effluent KW - Marine bivalve KW - Oxidative stress KW - Sewage treatment plant SP - 78 EP - 85 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 111 N2 - The biochemical effects in marine bivalves exposed to increasing concentrations of treated municipal effluent (TME), as discharged into receiving marine waters, are investigated. The effluent was collected from a municipal sewage treatment plant (STP) in Qingdao (China). Meretrix meretrix were exposed to effluent volume ratio (EVR, ratio of effluent volume accounted for tailwater seawater mixture) 0%, 1%, 5%, 10%, and 20% (v/v) TME for 15 days and the following biochemical responses in gills were measured: (1) the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione (GSH) content, and lipid peroxidation levels of malondialdehyde (MDA), as oxidative stress biomarkers; (2) the activity of 7-ethoxyresorufin O-deethylase (EROD) and gluthathione S-transferase (GST), as phase I and phase II conjugation enzymes, respectively; (3) acetylcholinesterase (AChE), as a biomarker of neurotoxicity, and (4) metallothioneins (MTs), as proteins strongly induced by heavy metals. Most of the biochemical indices present high and significant variation frequency (above 50%). There is enhancement in the antioxidant enzymes, EROD, GST, AChE, and MTs, as well as consumption of GSH. The current experimental results suggest that effluent with concentrations less than 20% (v/v) do not cause lipid peroxidation damage. This implies that the activated defense is sufficient to protect the bivalves' gill tissues from cytotoxicity produced by the effluent. Furthermore, GSH, GPx, MTs, and GR are suitable, and sufficiently sensitive, biomarkers to indicate the pollution levels in marine environments receiving such effluent. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/25450918/Biochemical_responses_in_the_gills_of_Meretrix_meretrix_after_exposure_to_treated_municipal_effluent_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(14)00468-0 DB - PRIME DP - Unbound Medicine ER -