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Neuroprotective effects of macranthoin G from Eucommia ulmoides against hydrogen peroxide-induced apoptosis in PC12 cells via inhibiting NF-κB activation.
Chem Biol Interact. 2014 Dec 05; 224:108-16.CB

Abstract

Oxidative stress-mediated cellular injury has been considered as a major cause of neurodegenerative diseases including Alzheimer and Parkinson diseases. The scavenging of reactive oxygen species (ROS) mediated by antioxidants may be a potential strategy for retarding the disease's progression. Macranthoin G (MCG), isolated from Eucommia ulmoides, is a derivative from chlorogenic acid methyl ester and caffeic acid. This study is aimed to investigate the protective role of MCG against the cytotoxicity induced by hydrogen peroxide (H2O2) and to elucidate potential protective mechanisms in rat pheochromocytoma (PC12) cells. The results showed that the treatment of PC12 cells with MCG prior to H2O2 exposure effectively increased the cell viability, and stabilized the mitochondria membrane potential (MMP); furthermore, it enhanced the antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) and the levels of intracellular glutathione (GSH); it also decreased the malondialdehyde (MDA) content, intracellular ROS, caspase-3 activation, as well as cell apoptosis. In addition, the MCG treatment minimized the cell injury by H2O2 via down-regulation of the NF-κB pathway as well as activation of phosphorylation of IκBα, p38, and the extracellular signal-regulated kinase (ERK). These results showed that that MCG is promising as a potential therapeutic agent for neurodegenerative diseases induced by oxidative damage and should be encouraged for further research.

Authors+Show Affiliations

Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, School of Life Sciences, Huaiyin Normal University, Huaian 223300, China.Department of Urology, Huai'an First People's Hospital, Nanjing Medical University, Huaian 223300, China.Institute of Agro-food Science and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.Institute of Agro-food Science and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.Tianjin Key Laboratory of Pulp & Paper, College of Material Science & Chemical Engineering, Tianjin University of Science & Technology, Tianjin 300457, China. Electronic address: sichli@tust.edu.cn.Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, School of Life Sciences, Huaiyin Normal University, Huaian 223300, China; College of Food Science and Pharmacology, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China.Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China.College of Food Science and Pharmacology, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China.College of Food Science and Pharmacology, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China.Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, School of Life Sciences, Huaiyin Normal University, Huaian 223300, China. Electronic address: wangxf@hytc.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25451577

Citation

Hu, Weicheng, et al. "Neuroprotective Effects of Macranthoin G From Eucommia Ulmoides Against Hydrogen Peroxide-induced Apoptosis in PC12 Cells Via Inhibiting NF-κB Activation." Chemico-biological Interactions, vol. 224, 2014, pp. 108-16.
Hu W, Wang G, Li P, et al. Neuroprotective effects of macranthoin G from Eucommia ulmoides against hydrogen peroxide-induced apoptosis in PC12 cells via inhibiting NF-κB activation. Chem Biol Interact. 2014;224:108-16.
Hu, W., Wang, G., Li, P., Wang, Y., Si, C. L., He, J., Long, W., Bai, Y., Feng, Z., & Wang, X. (2014). Neuroprotective effects of macranthoin G from Eucommia ulmoides against hydrogen peroxide-induced apoptosis in PC12 cells via inhibiting NF-κB activation. Chemico-biological Interactions, 224, 108-16. https://doi.org/10.1016/j.cbi.2014.10.011
Hu W, et al. Neuroprotective Effects of Macranthoin G From Eucommia Ulmoides Against Hydrogen Peroxide-induced Apoptosis in PC12 Cells Via Inhibiting NF-κB Activation. Chem Biol Interact. 2014 Dec 5;224:108-16. PubMed PMID: 25451577.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effects of macranthoin G from Eucommia ulmoides against hydrogen peroxide-induced apoptosis in PC12 cells via inhibiting NF-κB activation. AU - Hu,Weicheng, AU - Wang,Gongcheng, AU - Li,Pengxia, AU - Wang,Yuning, AU - Si,Chuan-Ling, AU - He,Jing, AU - Long,Wei, AU - Bai,Yujia, AU - Feng,Zuoshan, AU - Wang,Xinfeng, Y1 - 2014/10/19/ PY - 2014/06/18/received PY - 2014/09/15/revised PY - 2014/10/10/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2017/2/14/medline KW - Antioxidant enzyme KW - Macranthoin G KW - Malondialdehyde KW - Mitochondria membrane potential KW - Reactive oxygen species SP - 108 EP - 16 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 224 N2 - Oxidative stress-mediated cellular injury has been considered as a major cause of neurodegenerative diseases including Alzheimer and Parkinson diseases. The scavenging of reactive oxygen species (ROS) mediated by antioxidants may be a potential strategy for retarding the disease's progression. Macranthoin G (MCG), isolated from Eucommia ulmoides, is a derivative from chlorogenic acid methyl ester and caffeic acid. This study is aimed to investigate the protective role of MCG against the cytotoxicity induced by hydrogen peroxide (H2O2) and to elucidate potential protective mechanisms in rat pheochromocytoma (PC12) cells. The results showed that the treatment of PC12 cells with MCG prior to H2O2 exposure effectively increased the cell viability, and stabilized the mitochondria membrane potential (MMP); furthermore, it enhanced the antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) and the levels of intracellular glutathione (GSH); it also decreased the malondialdehyde (MDA) content, intracellular ROS, caspase-3 activation, as well as cell apoptosis. In addition, the MCG treatment minimized the cell injury by H2O2 via down-regulation of the NF-κB pathway as well as activation of phosphorylation of IκBα, p38, and the extracellular signal-regulated kinase (ERK). These results showed that that MCG is promising as a potential therapeutic agent for neurodegenerative diseases induced by oxidative damage and should be encouraged for further research. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/25451577/Neuroprotective_effects_of_macranthoin_G_from_Eucommia_ulmoides_against_hydrogen_peroxide_induced_apoptosis_in_PC12_cells_via_inhibiting_NF_κB_activation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(14)00299-3 DB - PRIME DP - Unbound Medicine ER -