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Passage-dependent relationship between mesenchymal stem cell mobilization and chondrogenic potential.
Osteoarthritis Cartilage 2015; 23(2):319-27OC

Abstract

OBJECTIVE

Galvanotaxis, the migratory response of cells in response to electrical stimulation, has been implicated in development and wound healing. The use of mesenchymal stem cells (MSCs) from the synovium (synovium-derived stem cells, SDSCs) has been investigated for repair strategies. Expansion of SDSCs is necessary to achieve clinically relevant cell numbers; however, the effects of culture passage on their subsequent cartilaginous extracellular matrix production are not well understood.

METHODS

Over four passages of SDSCs, we measured the expression of cell surface markers (CD31, CD34, CD49c, CD73) and assessed their migratory potential in response to applied direct current (DC) electric field. Cells from each passage were also used to form micropellets to assess the degree of cartilage-like tissue formation.

RESULTS

Expression of CD31, CD34, and CD49c remained constant throughout cell expansion; CD73 showed a transient increase through the first two passages. Correspondingly, we observed that early passage SDSCs exhibit anodal migration when subjected to applied DC electric field strength of 6 V/cm. By passage 3, CD73 expression significantly decreased; these cells exhibited cell migration toward the cathode, as previously observed for terminally differentiated chondrocytes. Only late passage cells (P4) were capable of developing cartilage-like tissue in micropellet culture.

CONCLUSIONS

Our results show cell priming protocols carried out for four passages selectively differentiate stem cells to behave like chondrocytes, both in their motility response to applied electric field and their production of cartilaginous tissue.

Authors+Show Affiliations

Department of Biomedical Engineering, Columbia University, 1210 Amsterdam Ave, New York, NY 10027, USA.Department of Biomedical Engineering, Columbia University, 1210 Amsterdam Ave, New York, NY 10027, USA.Department of Mechanical Engineering, University of California, Berkeley, 5122 Etcheverry Hall, Berkeley, CA 94720, USA.Department of Orthopaedic Surgery, St. Luke's-Roosevelt Hospital Center, 1000 10th Ave, New York, NY 10019, USA.Department of Orthopaedic Surgery, Brown University, 100 Butler Drive, Providence, RI 02906, USA.Department of Biomedical Engineering, Columbia University, 1210 Amsterdam Ave, New York, NY 10027, USA.Department of Biological Sciences, Columbia University, 1212 Amsterdam Ave, New York, NY 10027, USA.Department of Biomedical Engineering, Columbia University, 1210 Amsterdam Ave, New York, NY 10027, USA; Department of Mechanical Engineering, Columbia University, 500 W. 120th St, New York, NY 10027, USA.Department of Biomedical Engineering, Columbia University, 1210 Amsterdam Ave, New York, NY 10027, USA. Electronic address: cth6@columbia.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

25452155

Citation

Tan, A R., et al. "Passage-dependent Relationship Between Mesenchymal Stem Cell Mobilization and Chondrogenic Potential." Osteoarthritis and Cartilage, vol. 23, no. 2, 2015, pp. 319-27.
Tan AR, Alegre-Aguarón E, O'Connell GD, et al. Passage-dependent relationship between mesenchymal stem cell mobilization and chondrogenic potential. Osteoarthr Cartil. 2015;23(2):319-27.
Tan, A. R., Alegre-Aguarón, E., O'Connell, G. D., VandenBerg, C. D., Aaron, R. K., Vunjak-Novakovic, G., ... Hung, C. T. (2015). Passage-dependent relationship between mesenchymal stem cell mobilization and chondrogenic potential. Osteoarthritis and Cartilage, 23(2), pp. 319-27. doi:10.1016/j.joca.2014.10.001.
Tan AR, et al. Passage-dependent Relationship Between Mesenchymal Stem Cell Mobilization and Chondrogenic Potential. Osteoarthr Cartil. 2015;23(2):319-27. PubMed PMID: 25452155.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Passage-dependent relationship between mesenchymal stem cell mobilization and chondrogenic potential. AU - Tan,A R, AU - Alegre-Aguarón,E, AU - O'Connell,G D, AU - VandenBerg,C D, AU - Aaron,R K, AU - Vunjak-Novakovic,G, AU - Chloe Bulinski,J, AU - Ateshian,G A, AU - Hung,C T, Y1 - 2014/10/17/ PY - 2014/04/01/received PY - 2014/10/03/revised PY - 2014/10/06/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/7/24/medline KW - CD73 KW - Cartilage repair KW - Galvanotaxis KW - SDSCs KW - Tissue engineering SP - 319 EP - 27 JF - Osteoarthritis and cartilage JO - Osteoarthr. Cartil. VL - 23 IS - 2 N2 - OBJECTIVE: Galvanotaxis, the migratory response of cells in response to electrical stimulation, has been implicated in development and wound healing. The use of mesenchymal stem cells (MSCs) from the synovium (synovium-derived stem cells, SDSCs) has been investigated for repair strategies. Expansion of SDSCs is necessary to achieve clinically relevant cell numbers; however, the effects of culture passage on their subsequent cartilaginous extracellular matrix production are not well understood. METHODS: Over four passages of SDSCs, we measured the expression of cell surface markers (CD31, CD34, CD49c, CD73) and assessed their migratory potential in response to applied direct current (DC) electric field. Cells from each passage were also used to form micropellets to assess the degree of cartilage-like tissue formation. RESULTS: Expression of CD31, CD34, and CD49c remained constant throughout cell expansion; CD73 showed a transient increase through the first two passages. Correspondingly, we observed that early passage SDSCs exhibit anodal migration when subjected to applied DC electric field strength of 6 V/cm. By passage 3, CD73 expression significantly decreased; these cells exhibited cell migration toward the cathode, as previously observed for terminally differentiated chondrocytes. Only late passage cells (P4) were capable of developing cartilage-like tissue in micropellet culture. CONCLUSIONS: Our results show cell priming protocols carried out for four passages selectively differentiate stem cells to behave like chondrocytes, both in their motility response to applied electric field and their production of cartilaginous tissue. SN - 1522-9653 UR - https://www.unboundmedicine.com/medline/citation/25452155/Passage_dependent_relationship_between_mesenchymal_stem_cell_mobilization_and_chondrogenic_potential_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1063-4584(14)01279-5 DB - PRIME DP - Unbound Medicine ER -