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New immunization protocol to produce crotalic antivenom combining Crotalus durissus terrificus venom and its PLA2.
Biologicals. 2015 Jan; 43(1):62-70.B

Abstract

Antivenoms are usually obtained by animal immunization with successive inoculations of increasing sublethal amounts of venom, which may impair the animal health. The high lethality of venom requires prolonged immunization plans with small amounts of venom. Thus, we propose an alternative plan that includes a pre-immunization of the animal with phospholipase A2, the main crotoxin component, which is responsible for the whole venom lethality. For comparison, three different immunization schemes were designed: high dose protocol (HDP; 0.5-27 mg of venom), low dose protocol (LDP; 0.1-7 mg of venom) and Mix protocol (MP; preimmunization 0.1-1.2 mg of crotalic PLA2, and then 4.5-8 mg of venom). Antibody titers were determined by ELISA, in blood plasma obtained from the marginal vein of the ear. The neutralizing ability of the different sera obtained by all protocols (HDS, LDS and MS) was tested against the most important pharmacological activities of whole venom: PLA2 activity, myotoxicity, thrombin like activity and lethality. MS showed the best neutralizing efficacy and at the same time, it was obtained by an immunization protocol that takes account of animal health care, since it requires low quantities of venoms in comparison to traditional protocols.

Authors+Show Affiliations

Laboratorio de investigación en Proteínas (LabInPro), Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste (UNNE), Av. Libertad 5470, Corrientes 3400, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste (UNNE), Sargento Cabral 2139, Corrientes 3400, Argentina.Laboratorio de investigación en Proteínas (LabInPro), Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste (UNNE), Av. Libertad 5470, Corrientes 3400, Argentina.Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste (UNNE), Sargento Cabral 2139, Corrientes 3400, Argentina.Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste (UNNE), Sargento Cabral 2139, Corrientes 3400, Argentina.Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste (UNNE), Sargento Cabral 2139, Corrientes 3400, Argentina.Laboratorio de investigación en Proteínas (LabInPro), Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste (UNNE), Av. Libertad 5470, Corrientes 3400, Argentina. Electronic address: lauraleiva2004@yahoo.com.ar.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25453603

Citation

Fusco, Luciano Sebastián, et al. "New Immunization Protocol to Produce Crotalic Antivenom Combining Crotalus Durissus Terrificus Venom and Its PLA2." Biologicals : Journal of the International Association of Biological Standardization, vol. 43, no. 1, 2015, pp. 62-70.
Fusco LS, Rodríguez JP, Teibler P, et al. New immunization protocol to produce crotalic antivenom combining Crotalus durissus terrificus venom and its PLA2. Biologicals. 2015;43(1):62-70.
Fusco, L. S., Rodríguez, J. P., Teibler, P., Maruñak, S., Acosta, O., & Leiva, L. (2015). New immunization protocol to produce crotalic antivenom combining Crotalus durissus terrificus venom and its PLA2. Biologicals : Journal of the International Association of Biological Standardization, 43(1), 62-70. https://doi.org/10.1016/j.biologicals.2014.09.001
Fusco LS, et al. New Immunization Protocol to Produce Crotalic Antivenom Combining Crotalus Durissus Terrificus Venom and Its PLA2. Biologicals. 2015;43(1):62-70. PubMed PMID: 25453603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New immunization protocol to produce crotalic antivenom combining Crotalus durissus terrificus venom and its PLA2. AU - Fusco,Luciano Sebastián, AU - Rodríguez,Juan Pablo, AU - Teibler,Pamela, AU - Maruñak,Silvana, AU - Acosta,Ofelia, AU - Leiva,Laura, Y1 - 2014/11/08/ PY - 2014/05/26/received PY - 2014/09/06/revised PY - 2014/09/18/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/9/26/medline KW - Animal health care KW - Antivenom production KW - Crotalic envenomation KW - Phospholipase A(2) SP - 62 EP - 70 JF - Biologicals : journal of the International Association of Biological Standardization JO - Biologicals VL - 43 IS - 1 N2 - Antivenoms are usually obtained by animal immunization with successive inoculations of increasing sublethal amounts of venom, which may impair the animal health. The high lethality of venom requires prolonged immunization plans with small amounts of venom. Thus, we propose an alternative plan that includes a pre-immunization of the animal with phospholipase A2, the main crotoxin component, which is responsible for the whole venom lethality. For comparison, three different immunization schemes were designed: high dose protocol (HDP; 0.5-27 mg of venom), low dose protocol (LDP; 0.1-7 mg of venom) and Mix protocol (MP; preimmunization 0.1-1.2 mg of crotalic PLA2, and then 4.5-8 mg of venom). Antibody titers were determined by ELISA, in blood plasma obtained from the marginal vein of the ear. The neutralizing ability of the different sera obtained by all protocols (HDS, LDS and MS) was tested against the most important pharmacological activities of whole venom: PLA2 activity, myotoxicity, thrombin like activity and lethality. MS showed the best neutralizing efficacy and at the same time, it was obtained by an immunization protocol that takes account of animal health care, since it requires low quantities of venoms in comparison to traditional protocols. SN - 1095-8320 UR - https://www.unboundmedicine.com/medline/citation/25453603/New_immunization_protocol_to_produce_crotalic_antivenom_combining_Crotalus_durissus_terrificus_venom_and_its_PLA2_ DB - PRIME DP - Unbound Medicine ER -