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Identification and characterization of B-cell epitopes of 3FTx and PLA(2) toxins from Micrurus corallinus snake venom.
Toxicon. 2015 Jan; 93:51-60.T

Abstract

The main goal of this work was to develop a strategy to identify B-cell epitopes on four different three finger toxins (3FTX) and one phospholipase A2 (PLA2) from Micrurus corallinus snake venom. 3FTx and PLA2 are highly abundant components in Elapidic venoms and are the major responsibles for the toxicity observed in envenomation by coral snakes. Overlapping peptides from the sequence of each toxin were prepared by SPOT method and three different anti-elapidic sera were used to map the epitopes. After immunogenicity analysis of the spot-reactive peptides by EPITOPIA, a computational method, nine sequences from the five toxins were chemically synthesized and antigenically and immunogenically characterized. All the peptides were used together as immunogens in rabbits, delivered with Freund's adjuvant for a first cycle of immunization and Montanide in the second. A good antibody response against individual synthetic peptides and M. corallinus venom was achieved. Anti-peptide IgGs were also cross-reactive against Micrurus frontalis and Micrurus lemniscatus crude venoms. In addition, anti-peptide IgGs inhibits the lethal and phospholipasic activities of M. corallinus crude venom. Our results provide a rational basis to the identification of neutralizing epitopes on coral snake toxins and show that their corresponding synthetic peptides could improve the generation of immuno-therapeutics. The use of synthetic peptide for immunization is a reasonable approach, since it enables poly-specificity, low risk of toxic effects and large scale production.

Authors+Show Affiliations

Departamento de Bioquímica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Departamento de Bioquímica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil, 1500, 05503-900, São Paulo, SP, Brazil.Departamento de Bioquímica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Departamento de Bioquímica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Departamento de Bioquímica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Fundação Ezequiel Dias, Belo Horizonte, Brazil.Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil, 1500, 05503-900, São Paulo, SP, Brazil.Departamento de Bioquímica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: olortegi@icb.ufmg.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25454319

Citation

Castro, K L., et al. "Identification and Characterization of B-cell Epitopes of 3FTx and PLA(2) Toxins From Micrurus Corallinus Snake Venom." Toxicon : Official Journal of the International Society On Toxinology, vol. 93, 2015, pp. 51-60.
Castro KL, Duarte CG, Ramos HR, et al. Identification and characterization of B-cell epitopes of 3FTx and PLA(2) toxins from Micrurus corallinus snake venom. Toxicon. 2015;93:51-60.
Castro, K. L., Duarte, C. G., Ramos, H. R., Machado de Avila, R. A., Schneider, F. S., Oliveira, D., Freitas, C. F., Kalapothakis, E., Ho, P. L., & Chávez-Olortegui, C. (2015). Identification and characterization of B-cell epitopes of 3FTx and PLA(2) toxins from Micrurus corallinus snake venom. Toxicon : Official Journal of the International Society On Toxinology, 93, 51-60. https://doi.org/10.1016/j.toxicon.2014.10.015
Castro KL, et al. Identification and Characterization of B-cell Epitopes of 3FTx and PLA(2) Toxins From Micrurus Corallinus Snake Venom. Toxicon. 2015;93:51-60. PubMed PMID: 25454319.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of B-cell epitopes of 3FTx and PLA(2) toxins from Micrurus corallinus snake venom. AU - Castro,K L, AU - Duarte,C G, AU - Ramos,H R, AU - Machado de Avila,R A, AU - Schneider,F S, AU - Oliveira,D, AU - Freitas,C F, AU - Kalapothakis,E, AU - Ho,P L, AU - Chávez-Olortegui,C, Y1 - 2014/10/29/ PY - 2014/09/05/received PY - 2014/10/10/revised PY - 2014/10/15/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/8/27/medline KW - Antivenoms KW - Epitopes KW - Micrurus corallinus snake venom KW - Phospholipase A(2) (PLA(2)) KW - Synthetic peptides KW - Three-finger toxins (3FTx) SP - 51 EP - 60 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 93 N2 - The main goal of this work was to develop a strategy to identify B-cell epitopes on four different three finger toxins (3FTX) and one phospholipase A2 (PLA2) from Micrurus corallinus snake venom. 3FTx and PLA2 are highly abundant components in Elapidic venoms and are the major responsibles for the toxicity observed in envenomation by coral snakes. Overlapping peptides from the sequence of each toxin were prepared by SPOT method and three different anti-elapidic sera were used to map the epitopes. After immunogenicity analysis of the spot-reactive peptides by EPITOPIA, a computational method, nine sequences from the five toxins were chemically synthesized and antigenically and immunogenically characterized. All the peptides were used together as immunogens in rabbits, delivered with Freund's adjuvant for a first cycle of immunization and Montanide in the second. A good antibody response against individual synthetic peptides and M. corallinus venom was achieved. Anti-peptide IgGs were also cross-reactive against Micrurus frontalis and Micrurus lemniscatus crude venoms. In addition, anti-peptide IgGs inhibits the lethal and phospholipasic activities of M. corallinus crude venom. Our results provide a rational basis to the identification of neutralizing epitopes on coral snake toxins and show that their corresponding synthetic peptides could improve the generation of immuno-therapeutics. The use of synthetic peptide for immunization is a reasonable approach, since it enables poly-specificity, low risk of toxic effects and large scale production. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/25454319/Identification_and_characterization_of_B_cell_epitopes_of_3FTx_and_PLA_2__toxins_from_Micrurus_corallinus_snake_venom_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(14)00346-8 DB - PRIME DP - Unbound Medicine ER -