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Oxidative inhibition of Ca2+-ATPase in the rabbit lens.
Invest Ophthalmol Vis Sci. 1989 Jul; 30(7):1633-7.IO

Abstract

Hydrogen peroxide inhibition of maximum Ca2+-ATPase and Na+,K+-ATPase activity was measured in a membrane-enriched preparation of rabbit lens cortical fibers and epithelium. At 5 X 10(-6) M hydrogen peroxide maximum Ca2+-ATPase activity was inhibited by 39%, while maximum Na+,K+-ATPase activity was stimulated. Ca2+-ATPase activity was almost completely inhibited at 5 X 10(-4) M hydrogen peroxide, in comparison to Na+,K+-ATPase activity, which was only inhibited by 28% at a concentration of hydrogen peroxide an order of magnitude larger. The addition of catalase to hydrogen peroxide-pretreated samples did not reverse the inhibition of Ca2+-ATPase by hydrogen peroxide.

Authors+Show Affiliations

Department of Ophthalmology, University of Louisville School of Medicine, Kentucky Lions Eye Research Institute, Kentucky 40202.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2545647

Citation

Borchman, D, et al. "Oxidative Inhibition of Ca2+-ATPase in the Rabbit Lens." Investigative Ophthalmology & Visual Science, vol. 30, no. 7, 1989, pp. 1633-7.
Borchman D, Paterson CA, Delamere NA. Oxidative inhibition of Ca2+-ATPase in the rabbit lens. Invest Ophthalmol Vis Sci. 1989;30(7):1633-7.
Borchman, D., Paterson, C. A., & Delamere, N. A. (1989). Oxidative inhibition of Ca2+-ATPase in the rabbit lens. Investigative Ophthalmology & Visual Science, 30(7), 1633-7.
Borchman D, Paterson CA, Delamere NA. Oxidative Inhibition of Ca2+-ATPase in the Rabbit Lens. Invest Ophthalmol Vis Sci. 1989;30(7):1633-7. PubMed PMID: 2545647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative inhibition of Ca2+-ATPase in the rabbit lens. AU - Borchman,D, AU - Paterson,C A, AU - Delamere,N A, PY - 1989/7/1/pubmed PY - 1989/7/1/medline PY - 1989/7/1/entrez SP - 1633 EP - 7 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 30 IS - 7 N2 - Hydrogen peroxide inhibition of maximum Ca2+-ATPase and Na+,K+-ATPase activity was measured in a membrane-enriched preparation of rabbit lens cortical fibers and epithelium. At 5 X 10(-6) M hydrogen peroxide maximum Ca2+-ATPase activity was inhibited by 39%, while maximum Na+,K+-ATPase activity was stimulated. Ca2+-ATPase activity was almost completely inhibited at 5 X 10(-4) M hydrogen peroxide, in comparison to Na+,K+-ATPase activity, which was only inhibited by 28% at a concentration of hydrogen peroxide an order of magnitude larger. The addition of catalase to hydrogen peroxide-pretreated samples did not reverse the inhibition of Ca2+-ATPase by hydrogen peroxide. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/2545647/Oxidative_inhibition_of_Ca2+_ATPase_in_the_rabbit_lens_ L2 - https://iovs.arvojournals.org/article.aspx?volume=30&issue=7&page=1633 DB - PRIME DP - Unbound Medicine ER -