Tags

Type your tag names separated by a space and hit enter

Evidence for amphithallism and broad geographical hybridization potential among Agaricus subrufescens isolates from Brazil, France, and Thailand.
Fungal Biol. 2014 Dec; 118(12):1013-23.FB

Abstract

Agaricus subrufescens is a cultivated edible and medicinal mushroom. Its known geographical distribution encompasses the Americas, Europe, Oceania, and Asia. The objective of this study was to assess mating compatibility and interfertility of strains originating from Brazil, France, and Thailand. Progeny of each strain were analyed with codominant molecular markers. Multilocus genotype tests revealed that the three strains were amphithallic with percentages of heterokaryotic single spore progenies of 75% for the Thai strain and around 40% for the Brazilian and French strains. In mating tests A. subrufescens had a multiallelic unifactorial system of sexual incompatibility. The three parent strains were interfertile based on experimental pairings of single-spore isolates, the recovery of hybrid heterokaryons from compatible matings, and the ability of hybrids to produce mushrooms and fertile spores. This biological approach supports the inclusion of the European strains within the species and the extension of the geographical distribution range to Asia. Our data should help to develop breeding strategies and to better manage and exploit the diversity existing in A. subrufescens.

Authors+Show Affiliations

Institute of Excellence in Fungal Research, and School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.INRA, UR1264 MycSA, Mycologie et sécurité des aliments, CS 20032, 33883 Villenave d'Ornon Cedex, France.Aurora University, Biology Department, 347 S. Gladstone Ave., Aurora, IL 60506-4892, USA.Institute of Excellence in Fungal Research, and School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.INRA, UR1264 MycSA, Mycologie et sécurité des aliments, CS 20032, 33883 Villenave d'Ornon Cedex, France.Institute of Excellence in Fungal Research, and School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.Sylvan Biosciences, 198 Nolte Drive, Kittanning, PA 16201, USA.INRA, UR1264 MycSA, Mycologie et sécurité des aliments, CS 20032, 33883 Villenave d'Ornon Cedex, France.INRA, UR1264 MycSA, Mycologie et sécurité des aliments, CS 20032, 33883 Villenave d'Ornon Cedex, France. Electronic address: callac@bordeaux.inra.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25457949

Citation

Thongklang, Naritsada, et al. "Evidence for Amphithallism and Broad Geographical Hybridization Potential Among Agaricus Subrufescens Isolates From Brazil, France, and Thailand." Fungal Biology, vol. 118, no. 12, 2014, pp. 1013-23.
Thongklang N, Hoang E, Rodriguez Estrada AE, et al. Evidence for amphithallism and broad geographical hybridization potential among Agaricus subrufescens isolates from Brazil, France, and Thailand. Fungal Biol. 2014;118(12):1013-23.
Thongklang, N., Hoang, E., Rodriguez Estrada, A. E., Sysouphanthong, P., Moinard, M., Hyde, K. D., Kerrigan, R. W., Foulongne-Oriol, M., & Callac, P. (2014). Evidence for amphithallism and broad geographical hybridization potential among Agaricus subrufescens isolates from Brazil, France, and Thailand. Fungal Biology, 118(12), 1013-23. https://doi.org/10.1016/j.funbio.2014.10.004
Thongklang N, et al. Evidence for Amphithallism and Broad Geographical Hybridization Potential Among Agaricus Subrufescens Isolates From Brazil, France, and Thailand. Fungal Biol. 2014;118(12):1013-23. PubMed PMID: 25457949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for amphithallism and broad geographical hybridization potential among Agaricus subrufescens isolates from Brazil, France, and Thailand. AU - Thongklang,Naritsada, AU - Hoang,Eric, AU - Rodriguez Estrada,Alma E, AU - Sysouphanthong,Phongeun, AU - Moinard,Magalie, AU - Hyde,Kevin D, AU - Kerrigan,Richard W, AU - Foulongne-Oriol,Marie, AU - Callac,Philippe, Y1 - 2014/10/27/ PY - 2014/05/28/received PY - 2014/10/09/revised PY - 2014/10/17/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/7/28/medline KW - Interfertility KW - Life cycle KW - Meiosporogenesis KW - Postmeiotic mitosis KW - Pseudohomothallism SP - 1013 EP - 23 JF - Fungal biology JO - Fungal Biol VL - 118 IS - 12 N2 - Agaricus subrufescens is a cultivated edible and medicinal mushroom. Its known geographical distribution encompasses the Americas, Europe, Oceania, and Asia. The objective of this study was to assess mating compatibility and interfertility of strains originating from Brazil, France, and Thailand. Progeny of each strain were analyed with codominant molecular markers. Multilocus genotype tests revealed that the three strains were amphithallic with percentages of heterokaryotic single spore progenies of 75% for the Thai strain and around 40% for the Brazilian and French strains. In mating tests A. subrufescens had a multiallelic unifactorial system of sexual incompatibility. The three parent strains were interfertile based on experimental pairings of single-spore isolates, the recovery of hybrid heterokaryons from compatible matings, and the ability of hybrids to produce mushrooms and fertile spores. This biological approach supports the inclusion of the European strains within the species and the extension of the geographical distribution range to Asia. Our data should help to develop breeding strategies and to better manage and exploit the diversity existing in A. subrufescens. SN - 1878-6146 UR - https://www.unboundmedicine.com/medline/citation/25457949/Evidence_for_amphithallism_and_broad_geographical_hybridization_potential_among_Agaricus_subrufescens_isolates_from_Brazil_France_and_Thailand_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1878-6146(14)00157-3 DB - PRIME DP - Unbound Medicine ER -