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Antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of Calotropis gigantea L.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Feb 05; 136 Pt B:924-30.SA

Abstract

The present study aimed to synthesis silver nanoparticles (AgNPs) in a greener route using aqueous latex extract of Calotropis gigantea L. toward biomedical applications. Initially, synthesis of AgNPs was confirmed through UV-Vis spectroscopy which shows the surface plasmonic resonance peak (SPR) at 420 nm. Fourier transform infrared spectroscopy (FTIR) analysis provides clear evidence that protein fractions present in the latex extract act as reducing and stabilizing bio agents. Energy dispersive X-ray (EDAX) spectroscopy confirms the presence of silver as a major constituent element. X-ray diffractograms displays that the synthesized AgNPs were biphasic crystalline nature. Electron microscopic studies such as Field emission scanning electron microscopic (Fe-SEM) and Transmission electron microscope (TEM) reveals that synthesized AgNPs are spherical in shape with the size range between 5 and 30 nm. Further, crude latex aqueous extract and synthesized AgNPs were evaluated against different bacterial pathogens such as Bacillus cereus, Enterococci sp, Shigella sp, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus and Escherichia coli. Compared to the crude latex aqueous extract, biosynthesized AgNPs exhibits a remarkable antimicrobial activity. Likewise in vitro anticancer study manifests the cytotoxicity value of synthesized AgNPs against tested HeLa cells. The output of this study clearly suggesting that biosynthesized AgNPs using latex of C. gigantea can be used as promising nanomaterial for therapeutic application in context with nanodrug formulation.

Authors+Show Affiliations

Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India. Electronic address: sivaramakrishnan123@yahoo.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25459618

Citation

Rajkuberan, Chandrasekaran, et al. "Antibacterial and Cytotoxic Potential of Silver Nanoparticles Synthesized Using Latex of Calotropis Gigantea L." Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, vol. 136 Pt B, 2015, pp. 924-30.
Rajkuberan C, Sudha K, Sathishkumar G, et al. Antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of Calotropis gigantea L. Spectrochim Acta A Mol Biomol Spectrosc. 2015;136 Pt B:924-30.
Rajkuberan, C., Sudha, K., Sathishkumar, G., & Sivaramakrishnan, S. (2015). Antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of Calotropis gigantea L. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 136 Pt B, 924-30. https://doi.org/10.1016/j.saa.2014.09.115
Rajkuberan C, et al. Antibacterial and Cytotoxic Potential of Silver Nanoparticles Synthesized Using Latex of Calotropis Gigantea L. Spectrochim Acta A Mol Biomol Spectrosc. 2015 Feb 5;136 Pt B:924-30. PubMed PMID: 25459618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of Calotropis gigantea L. AU - Rajkuberan,Chandrasekaran, AU - Sudha,Kannaiah, AU - Sathishkumar,Gnanasekar, AU - Sivaramakrishnan,Sivaperumal, Y1 - 2014/10/05/ PY - 2014/06/10/received PY - 2014/08/18/revised PY - 2014/09/28/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/8/8/medline KW - Calotropis gigantea L. KW - Cytotoxicity KW - SPR KW - Silver nanoparticles KW - XRD SP - 924 EP - 30 JF - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JO - Spectrochim Acta A Mol Biomol Spectrosc VL - 136 Pt B N2 - The present study aimed to synthesis silver nanoparticles (AgNPs) in a greener route using aqueous latex extract of Calotropis gigantea L. toward biomedical applications. Initially, synthesis of AgNPs was confirmed through UV-Vis spectroscopy which shows the surface plasmonic resonance peak (SPR) at 420 nm. Fourier transform infrared spectroscopy (FTIR) analysis provides clear evidence that protein fractions present in the latex extract act as reducing and stabilizing bio agents. Energy dispersive X-ray (EDAX) spectroscopy confirms the presence of silver as a major constituent element. X-ray diffractograms displays that the synthesized AgNPs were biphasic crystalline nature. Electron microscopic studies such as Field emission scanning electron microscopic (Fe-SEM) and Transmission electron microscope (TEM) reveals that synthesized AgNPs are spherical in shape with the size range between 5 and 30 nm. Further, crude latex aqueous extract and synthesized AgNPs were evaluated against different bacterial pathogens such as Bacillus cereus, Enterococci sp, Shigella sp, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus and Escherichia coli. Compared to the crude latex aqueous extract, biosynthesized AgNPs exhibits a remarkable antimicrobial activity. Likewise in vitro anticancer study manifests the cytotoxicity value of synthesized AgNPs against tested HeLa cells. The output of this study clearly suggesting that biosynthesized AgNPs using latex of C. gigantea can be used as promising nanomaterial for therapeutic application in context with nanodrug formulation. SN - 1873-3557 UR - https://www.unboundmedicine.com/medline/citation/25459618/Antibacterial_and_cytotoxic_potential_of_silver_nanoparticles_synthesized_using_latex_of_Calotropis_gigantea_L_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-1425(14)01473-5 DB - PRIME DP - Unbound Medicine ER -