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Terminalia catappa L.: a medicinal plant from the Caribbean pharmacopeia with anti-Helicobacter pylori and antiulcer action in experimental rodent models.
J Ethnopharmacol. 2015 Jan 15; 159:285-95.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Terminalia catappa L. (Combretaceae) is a medicinal plant listed as a pharmacopeia vegetable from Caribbean to treat gastritis. The objective of this study was to evaluate the gastroprotective and healing effect of the aqueous fraction (FrAq) obtained from the leaves of Terminalia catappa and to determine the antiulcer mechanism of action in experimental rodent models and its activity to Helicobacter pylori.

MATERIAL AND METHODS

In rodents, the FrAq was challenged by different necrotizing agents, such as absolute ethanol and ischemia-reperfusion injury. The antiulcer mechanism of action of FrAq was assessed and the healing effects of the fraction after seven and 14 days of treatment was evaluated by matrix metalloproteinase activity (MMP-2 and MMP-9). The toxicological effect of subacute treatment with FrAq during 14 days of treatment was also analyzed. The anti-Helicobacter pylori activity was determined by microdilution. The phytochemical study of the fraction was analyzed by experiments with FIA-ESI-IT-MS(n) (Direct Flow Analysis-ionization Electrospray Ion Trap Tandem Mass Spectrometry) and high performance liquid chromatography (HPLC) coupled to a photodiode array (PDA).

RESULTS

Oral treatment with FrAq (25mg/kg) significantly decreased the number of ulcerative lesions induced by ethanol and ischemia/reperfusion injury. The action of FrAq was mediated by the activation of defensive mucosa-protective factors, such as increases in mucus production, the nitric oxide (NO) pathway and endogenous prostaglandins. Oral treatment with FrAq for seven and 14 days significantly reduced the lesion area (80% and 37%, respectively) compared to the negative control group. Analyses of MMP-9 and MMP-2 activity from gastric mucosa confirmed the accelerated gastric healing effect of FrAq. This extract also presented considerable activity against Helicobacter pylori. The mass spectrum and MS/MS of the aqueous fraction indicates the existence of many different phenolic compounds, including punicalagin, punicalin, and gallagic acid, among others.

CONCLUSIONS

We concluded that FrAq from Terminalia catappa leaves has excellent preventive and curative effects on acute and chronic induced gastric ulcers and showed an important profile against Helicobacter pylori.

Authors+Show Affiliations

Univ. Estadual Paulista-UNESP - Departamento de Fisiologia, Instituto de Biociências, CEP 18618-970 Botucatu, SP, Brazil.Univ. Estadual Paulista-UNESP - Departamento de Fisiologia, Instituto de Biociências, CEP 18618-970 Botucatu, SP, Brazil.Univ. Estadual Paulista-UNESP - Departamento de Fisiologia, Instituto de Biociências, CEP 18618-970 Botucatu, SP, Brazil.Univ. Estadual Paulista-UNESP - Departamento de Fisiologia, Instituto de Biociências, CEP 18618-970 Botucatu, SP, Brazil.Univ. Estadual Paulista-UNESP - Departamento de Química Orgânica, Instituto de Química, CEP 14800-900, Araraquara, SP, Brazil.Univ. Estadual Paulista-UNESP - Departamento de Química Orgânica, Instituto de Química, CEP 14800-900, Araraquara, SP, Brazil.Univ. Estadual Paulista-UNESP - Departamento de Morfologia, Instituto de Biociências, CEP 18618-970, Botucatu, SP, Brazil.Univ. Estadual Paulista-UNESP - Departamento de Morfologia, Instituto de Biociências, CEP 18618-970, Botucatu, SP, Brazil.Univ. Estadual Paulista-UNESP - Campus Experimental do Litoral Paulista, CEP 11330-900 São Vicente, SP, Brazil.Univ. Estadual Paulista-UNESP - Departamento de Fisiologia, Instituto de Biociências, CEP 18618-970 Botucatu, SP, Brazil.Univ. Estadual Paulista-UNESP - Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, CEP 14801-902, Araraquara, SP, Brazil.Univ. Estadual Paulista-UNESP - Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, CEP 14801-902, Araraquara, SP, Brazil.Universidade Santa Cecília - Pós-Graduação em Sustentabilidade de Ecossistemas Costeiros e Marinhos, Rua Oswaldo Cruz, 266, Boqueirão, CEP 11045907 Santos, SP, Brazil. Electronic address: walbertoma@gmail.com.Univ. Estadual Paulista-UNESP - Departamento de Fisiologia, Instituto de Biociências, CEP 18618-970 Botucatu, SP, Brazil. Electronic address: hiruma@ibb.unesp.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25460589

Citation

Pinheiro Silva, Laísa, et al. "Terminalia Catappa L.: a Medicinal Plant From the Caribbean Pharmacopeia With anti-Helicobacter Pylori and Antiulcer Action in Experimental Rodent Models." Journal of Ethnopharmacology, vol. 159, 2015, pp. 285-95.
Pinheiro Silva L, Damacena de Angelis C, Bonamin F, et al. Terminalia catappa L.: a medicinal plant from the Caribbean pharmacopeia with anti-Helicobacter pylori and antiulcer action in experimental rodent models. J Ethnopharmacol. 2015;159:285-95.
Pinheiro Silva, L., Damacena de Angelis, C., Bonamin, F., Kushima, H., José Mininel, F., Campaner Dos Santos, L., Karina Delella, F., Luis Felisbino, S., Vilegas, W., Regina Machado da Rocha, L., Aparecido Dos Santos Ramos, M., Maria Bauab, T., Toma, W., & Akiko Hiruma-Lima, C. (2015). Terminalia catappa L.: a medicinal plant from the Caribbean pharmacopeia with anti-Helicobacter pylori and antiulcer action in experimental rodent models. Journal of Ethnopharmacology, 159, 285-95. https://doi.org/10.1016/j.jep.2014.11.025
Pinheiro Silva L, et al. Terminalia Catappa L.: a Medicinal Plant From the Caribbean Pharmacopeia With anti-Helicobacter Pylori and Antiulcer Action in Experimental Rodent Models. J Ethnopharmacol. 2015 Jan 15;159:285-95. PubMed PMID: 25460589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Terminalia catappa L.: a medicinal plant from the Caribbean pharmacopeia with anti-Helicobacter pylori and antiulcer action in experimental rodent models. AU - Pinheiro Silva,Laísa, AU - Damacena de Angelis,Célio, AU - Bonamin,Flavia, AU - Kushima,Hélio, AU - José Mininel,Francisco, AU - Campaner Dos Santos,Lourdes, AU - Karina Delella,Flavia, AU - Luis Felisbino,Sergio, AU - Vilegas,Wagner, AU - Regina Machado da Rocha,Lucia, AU - Aparecido Dos Santos Ramos,Matheus, AU - Maria Bauab,Tais, AU - Toma,Walber, AU - Akiko Hiruma-Lima,Clelia, Y1 - 2014/11/24/ PY - 2014/08/22/received PY - 2014/11/03/revised PY - 2014/11/13/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/9/5/medline KW - Combretaceae KW - Gastric healing action KW - Helicobacter pylori KW - MMP-2 KW - MMP-9 KW - Terminalia catappa L. SP - 285 EP - 95 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 159 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Terminalia catappa L. (Combretaceae) is a medicinal plant listed as a pharmacopeia vegetable from Caribbean to treat gastritis. The objective of this study was to evaluate the gastroprotective and healing effect of the aqueous fraction (FrAq) obtained from the leaves of Terminalia catappa and to determine the antiulcer mechanism of action in experimental rodent models and its activity to Helicobacter pylori. MATERIAL AND METHODS: In rodents, the FrAq was challenged by different necrotizing agents, such as absolute ethanol and ischemia-reperfusion injury. The antiulcer mechanism of action of FrAq was assessed and the healing effects of the fraction after seven and 14 days of treatment was evaluated by matrix metalloproteinase activity (MMP-2 and MMP-9). The toxicological effect of subacute treatment with FrAq during 14 days of treatment was also analyzed. The anti-Helicobacter pylori activity was determined by microdilution. The phytochemical study of the fraction was analyzed by experiments with FIA-ESI-IT-MS(n) (Direct Flow Analysis-ionization Electrospray Ion Trap Tandem Mass Spectrometry) and high performance liquid chromatography (HPLC) coupled to a photodiode array (PDA). RESULTS: Oral treatment with FrAq (25mg/kg) significantly decreased the number of ulcerative lesions induced by ethanol and ischemia/reperfusion injury. The action of FrAq was mediated by the activation of defensive mucosa-protective factors, such as increases in mucus production, the nitric oxide (NO) pathway and endogenous prostaglandins. Oral treatment with FrAq for seven and 14 days significantly reduced the lesion area (80% and 37%, respectively) compared to the negative control group. Analyses of MMP-9 and MMP-2 activity from gastric mucosa confirmed the accelerated gastric healing effect of FrAq. This extract also presented considerable activity against Helicobacter pylori. The mass spectrum and MS/MS of the aqueous fraction indicates the existence of many different phenolic compounds, including punicalagin, punicalin, and gallagic acid, among others. CONCLUSIONS: We concluded that FrAq from Terminalia catappa leaves has excellent preventive and curative effects on acute and chronic induced gastric ulcers and showed an important profile against Helicobacter pylori. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/25460589/Terminalia_catappa_L_:_a_medicinal_plant_from_the_Caribbean_pharmacopeia_with_anti_Helicobacter_pylori_and_antiulcer_action_in_experimental_rodent_models_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(14)00809-5 DB - PRIME DP - Unbound Medicine ER -