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SELDI-TOF MS-based discovery of a biomarker in Cucumis sativus seeds exposed to CuO nanoparticles.
Environ Toxicol Pharmacol. 2014 Nov; 38(3):922-31.ET

Abstract

Metal oxide nanoparticles (NPs) can inhibit plant seed germination and root elongation via the release of metal ions. In the present study, two acute phytotoxicity tests, seed germination and root elongation tests, were conducted on cucumber seeds (Cucumis sativus) treated with bulk copper oxide (CuO) and CuO NPs. Two concentrations of bulk CuO and CuO NPs, 200 and 600ppm, were used to test the inhibition rate of root germination; both concentrations of bulk CuO weakly inhibited seed germination, whereas CuO NPs significantly inhibited germination, showing a low germination rate of 23.3% at 600ppm. Root elongation tests demonstrated that CuO NPs were much stronger inhibitors than bulk CuO. SELDI-TOF MS analysis showed that 34 proteins were differentially expressed in cucumber seeds after exposure to CuO NPs, with the expression patterns of at least 9 proteins highly differing from those in seeds treated with bulk CuO and in control plants. Therefore, these 9 proteins were used to identify CuO NP-specific biomarkers in cucumber plants exposed to CuO NPs. A 5977-m/z protein was the most distinguishable biomarker for determining phytotoxicity by CuO NPs. Principal component analysis (PCA) of the SELDI-TOF MS results showed variability in the modes of inhibitory action on cucumber seeds and roots. To our knowledge, this is the first study to demonstrate that the phytotoxic effect of metal oxide NPs on plants is not caused by the same mode of action as other toxins.

Authors+Show Affiliations

School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea.School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea.Department of Environmental Engineering, Kumoh National Institute of Technology, Daehak-ro 61, Gumi, Gyeongbuk 730-701, Republic of Korea.Department of Bioenvironmental Chemistry and Institute of Agricultural Science and Technology, Chonbuk National University, Jeonju 561-756, Republic of Korea.School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic address: selpest@knu.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25461552

Citation

Moon, Young-Sun, et al. "SELDI-TOF MS-based Discovery of a Biomarker in Cucumis Sativus Seeds Exposed to CuO Nanoparticles." Environmental Toxicology and Pharmacology, vol. 38, no. 3, 2014, pp. 922-31.
Moon YS, Park ES, Kim TO, et al. SELDI-TOF MS-based discovery of a biomarker in Cucumis sativus seeds exposed to CuO nanoparticles. Environ Toxicol Pharmacol. 2014;38(3):922-31.
Moon, Y. S., Park, E. S., Kim, T. O., Lee, H. S., & Lee, S. E. (2014). SELDI-TOF MS-based discovery of a biomarker in Cucumis sativus seeds exposed to CuO nanoparticles. Environmental Toxicology and Pharmacology, 38(3), 922-31. https://doi.org/10.1016/j.etap.2014.10.002
Moon YS, et al. SELDI-TOF MS-based Discovery of a Biomarker in Cucumis Sativus Seeds Exposed to CuO Nanoparticles. Environ Toxicol Pharmacol. 2014;38(3):922-31. PubMed PMID: 25461552.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SELDI-TOF MS-based discovery of a biomarker in Cucumis sativus seeds exposed to CuO nanoparticles. AU - Moon,Young-Sun, AU - Park,Eun-Sil, AU - Kim,Tae-Oh, AU - Lee,Hoi-Seon, AU - Lee,Sung-Eun, Y1 - 2014/10/17/ PY - 2014/06/21/received PY - 2014/10/03/revised PY - 2014/10/06/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/8/15/medline KW - Biomarker KW - CuO nanoparticles KW - Phytotoxicity KW - SELDI-TOF MS SP - 922 EP - 31 JF - Environmental toxicology and pharmacology JO - Environ Toxicol Pharmacol VL - 38 IS - 3 N2 - Metal oxide nanoparticles (NPs) can inhibit plant seed germination and root elongation via the release of metal ions. In the present study, two acute phytotoxicity tests, seed germination and root elongation tests, were conducted on cucumber seeds (Cucumis sativus) treated with bulk copper oxide (CuO) and CuO NPs. Two concentrations of bulk CuO and CuO NPs, 200 and 600ppm, were used to test the inhibition rate of root germination; both concentrations of bulk CuO weakly inhibited seed germination, whereas CuO NPs significantly inhibited germination, showing a low germination rate of 23.3% at 600ppm. Root elongation tests demonstrated that CuO NPs were much stronger inhibitors than bulk CuO. SELDI-TOF MS analysis showed that 34 proteins were differentially expressed in cucumber seeds after exposure to CuO NPs, with the expression patterns of at least 9 proteins highly differing from those in seeds treated with bulk CuO and in control plants. Therefore, these 9 proteins were used to identify CuO NP-specific biomarkers in cucumber plants exposed to CuO NPs. A 5977-m/z protein was the most distinguishable biomarker for determining phytotoxicity by CuO NPs. Principal component analysis (PCA) of the SELDI-TOF MS results showed variability in the modes of inhibitory action on cucumber seeds and roots. To our knowledge, this is the first study to demonstrate that the phytotoxic effect of metal oxide NPs on plants is not caused by the same mode of action as other toxins. SN - 1872-7077 UR - https://www.unboundmedicine.com/medline/citation/25461552/SELDI_TOF_MS_based_discovery_of_a_biomarker_in_Cucumis_sativus_seeds_exposed_to_CuO_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1382-6689(14)00238-5 DB - PRIME DP - Unbound Medicine ER -