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Venomics and antivenomics of Bothrops erythromelas from five geographic populations within the Caatinga ecoregion of northeastern Brazil.
J Proteomics. 2015 Jan 30; 114:93-114.JP

Abstract

The Caatinga lancehead, Bothrops erythromelas, is a medically relevant species, responsible for most of the snakebite accidents in most parts of its distribution range in northeastern Brazil. The spectrum and geographic variability of its venom toxins were investigated applying a venomics approach to venom pools from five geographic areas within the Caatinga ecoregion. Despite its wide habitat, populations of B. erythromelas from Ceará, Pernambuco, Juazeiro, Paraiba, and Ilha de Itaparica exhibit highly conserved venom proteomes. Mirroring their compositional conservation, the five geographic venom pools also showed qualitatively and quantitatively overlapping antivenomic profiles against antivenoms generated in Vital Brazil (BR) and Clodomiro Picado (CR) Institutes, using different venoms in the immunization mixtures. The paraspecificity exhibited by the Brazilian SAB and the Costa Rican BCL antivenoms against venom toxins from B. erythromelas indicates large immunoreactive epitope conservation across genus Bothrops during the last ~14 million years, thus offering promise for the possibility of generating a broad-spectrum bothropic antivenom. Biological Significance Accidental snakebite envenomings represent an important public health hazard in Brazil. Ninety per cent of the yearly estimated 20-30,000 snakebite accidents are caused by species of the Bothrops genus. Bothrops erythromelas, a small, moderately stocky terrestrial venomous snake, is responsible for most of the snakebite accidents in its broad distribution range in the Caatinga, a large ecoregion in northeastern Brazil. To gain a deeper insight into the spectrum of medically important toxins present in the venom of the Caatinga lancehead, we applied a venomics approach to define the proteome and geographic variability of adult B. erythromelas venoms from five geographic regions. Although intraspecific compositional variation between venoms among specimens from different geographic regions has long been appreciated by herpetologists and toxinologists as a general feature of highly adaptable and widely distributed snake species, the five B. erythromelas populations investigated exhibit highly conserved venom proteomes. The overall toxin profile of the Caatinga lancehead's venom explains the local and systemic effects observed in envenomations by B. erythromelas. The five geographic venom pools sampled also showed qualitatively and quantitatively overlapping antivenomic profiles against antivenoms generated using different bothropic venoms in the immunization mixtures. The large immunoreactive epitope conservation across genus Bothrops offers promise for the generation of a broad-spectrum bothropic antivenom.

Authors+Show Affiliations

Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará (UFC), Fortaleza, 60430-270 Brasil; Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, 46010 Valencia, Spain.Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará (UFC), Fortaleza, 60430-270 Brasil.Instituto de Bioquímica Médica, Programa de Biologia Estrutural, Laboratório de Hemostase e Venenos, Universidade Federal do Rio de Janeiro (UFRJ), Brasil; Instituto Nacional de Biologia Estrutural e Bioimagem and Rede Proteomica do Rio de Janeiro, Universidade Federal do Rio de Janeiro (UFRJ), Brasil.Departamento de Zoologia, Universidade Federal de Pernambuco (UFPE), Recife, 50740-520 Brasil.Departamento de Antibióticos, Universidade Federal de Pernambuco (UFPE), Recife, 50740-520 Brasil.Departamento de Biologia, Universidade Federal do Ceará, Fortaleza, 60430-270 Brasil.Departamento de Biologia, Universidade Estadual da Paraíba (UEPB), Campina Grande, 58429-600 Brasil.Instituto de Bioquímica Médica, Programa de Biologia Estrutural, Laboratório de Hemostase e Venenos, Universidade Federal do Rio de Janeiro (UFRJ), Brasil; Instituto Nacional de Biologia Estrutural e Bioimagem and Rede Proteomica do Rio de Janeiro, Universidade Federal do Rio de Janeiro (UFRJ), Brasil.Instituto de Bioquímica Médica, Programa de Biologia Estrutural, Laboratório de Hemostase e Venenos, Universidade Federal do Rio de Janeiro (UFRJ), Brasil; Instituto Nacional de Biologia Estrutural e Bioimagem and Rede Proteomica do Rio de Janeiro, Universidade Federal do Rio de Janeiro (UFRJ), Brasil; Instituto Vital Brazil, Niterói, Rio de Janeiro, Brasil.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, 46010 Valencia, Spain.Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, 46010 Valencia, Spain. Electronic address: jcalvete@ibv.csic.es.Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, 46010 Valencia, Spain. Electronic address: dpla@ibv.csic.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25462430

Citation

Jorge, Roberta Jeane B., et al. "Venomics and Antivenomics of Bothrops Erythromelas From Five Geographic Populations Within the Caatinga Ecoregion of Northeastern Brazil." Journal of Proteomics, vol. 114, 2015, pp. 93-114.
Jorge RJ, Monteiro HS, Gonçalves-Machado L, et al. Venomics and antivenomics of Bothrops erythromelas from five geographic populations within the Caatinga ecoregion of northeastern Brazil. J Proteomics. 2015;114:93-114.
Jorge, R. J., Monteiro, H. S., Gonçalves-Machado, L., Guarnieri, M. C., Ximenes, R. M., Borges-Nojosa, D. M., Luna, K. P., Zingali, R. B., Corrêa-Netto, C., Gutiérrez, J. M., Sanz, L., Calvete, J. J., & Pla, D. (2015). Venomics and antivenomics of Bothrops erythromelas from five geographic populations within the Caatinga ecoregion of northeastern Brazil. Journal of Proteomics, 114, 93-114. https://doi.org/10.1016/j.jprot.2014.11.011
Jorge RJ, et al. Venomics and Antivenomics of Bothrops Erythromelas From Five Geographic Populations Within the Caatinga Ecoregion of Northeastern Brazil. J Proteomics. 2015 Jan 30;114:93-114. PubMed PMID: 25462430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Venomics and antivenomics of Bothrops erythromelas from five geographic populations within the Caatinga ecoregion of northeastern Brazil. AU - Jorge,Roberta Jeane B, AU - Monteiro,Helena S A, AU - Gonçalves-Machado,Larissa, AU - Guarnieri,Míriam C, AU - Ximenes,Rafael M, AU - Borges-Nojosa,Diva M, AU - Luna,Karla P de O, AU - Zingali,Russolina B, AU - Corrêa-Netto,Carlos, AU - Gutiérrez,José María, AU - Sanz,Libia, AU - Calvete,Juan J, AU - Pla,Davinia, Y1 - 2014/11/21/ PY - 2014/10/21/received PY - 2014/11/11/accepted PY - 2014/12/3/entrez PY - 2014/12/3/pubmed PY - 2015/10/2/medline KW - Antivenomics KW - Bothrops erythromelas KW - Bothrops neuwiedi complex KW - Caatinga lancehead KW - Snake venom proteomics SP - 93 EP - 114 JF - Journal of proteomics JO - J Proteomics VL - 114 N2 - The Caatinga lancehead, Bothrops erythromelas, is a medically relevant species, responsible for most of the snakebite accidents in most parts of its distribution range in northeastern Brazil. The spectrum and geographic variability of its venom toxins were investigated applying a venomics approach to venom pools from five geographic areas within the Caatinga ecoregion. Despite its wide habitat, populations of B. erythromelas from Ceará, Pernambuco, Juazeiro, Paraiba, and Ilha de Itaparica exhibit highly conserved venom proteomes. Mirroring their compositional conservation, the five geographic venom pools also showed qualitatively and quantitatively overlapping antivenomic profiles against antivenoms generated in Vital Brazil (BR) and Clodomiro Picado (CR) Institutes, using different venoms in the immunization mixtures. The paraspecificity exhibited by the Brazilian SAB and the Costa Rican BCL antivenoms against venom toxins from B. erythromelas indicates large immunoreactive epitope conservation across genus Bothrops during the last ~14 million years, thus offering promise for the possibility of generating a broad-spectrum bothropic antivenom. Biological Significance Accidental snakebite envenomings represent an important public health hazard in Brazil. Ninety per cent of the yearly estimated 20-30,000 snakebite accidents are caused by species of the Bothrops genus. Bothrops erythromelas, a small, moderately stocky terrestrial venomous snake, is responsible for most of the snakebite accidents in its broad distribution range in the Caatinga, a large ecoregion in northeastern Brazil. To gain a deeper insight into the spectrum of medically important toxins present in the venom of the Caatinga lancehead, we applied a venomics approach to define the proteome and geographic variability of adult B. erythromelas venoms from five geographic regions. Although intraspecific compositional variation between venoms among specimens from different geographic regions has long been appreciated by herpetologists and toxinologists as a general feature of highly adaptable and widely distributed snake species, the five B. erythromelas populations investigated exhibit highly conserved venom proteomes. The overall toxin profile of the Caatinga lancehead's venom explains the local and systemic effects observed in envenomations by B. erythromelas. The five geographic venom pools sampled also showed qualitatively and quantitatively overlapping antivenomic profiles against antivenoms generated using different bothropic venoms in the immunization mixtures. The large immunoreactive epitope conservation across genus Bothrops offers promise for the generation of a broad-spectrum bothropic antivenom. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/25462430/Venomics_and_antivenomics_of_Bothrops_erythromelas_from_five_geographic_populations_within_the_Caatinga_ecoregion_of_northeastern_Brazil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(14)00534-X DB - PRIME DP - Unbound Medicine ER -