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Bioactive amines in sorghum: method optimisation and influence of line, tannin and hydric stress.
Food Chem. 2015 Apr 15; 173:224-30.FC

Abstract

The profile and levels of bioactive amines in different sorghum lines were reported for the first time. The amines were quantified by ion-pair HPLC, post-column derivatisation with o-phthalaldehyde and fluorimetric detection. The extraction procedure was optimised: 420 μm particle size, extraction with 5% trichloroacetic acid and three extractions. The screening of 22 sorghum lines showed that four of the ten amines investigated were detected. Spermine and spermidine were the prevalent amines (100%), followed by putrescine (77%) and cadaverine (14%). Total amines ranged from 5.8 to 41.4 mg/100 g, and the polyamines represented 60-100% of the total. Sorghum without tannin had higher amines levels compared to sorghum with tannin and cadaverine was specific to samples without tannin. Hydric stress caused accumulation of spermidine in the grains and affected the levels of other amines at rates depending on the presence or not of tannin. Sorghum is a significant source of polyamines.

Authors+Show Affiliations

LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, bloco 3, sala 2091, Pampulha, CEP 31210-901 Belo Horizonte, MG, Brazil.LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, bloco 3, sala 2091, Pampulha, CEP 31210-901 Belo Horizonte, MG, Brazil.Embrapa Milho e Sorgo, Caixa Postal 151, CEP 35701-970 Sete Lagoas, MG, Brazil.LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, bloco 3, sala 2091, Pampulha, CEP 31210-901 Belo Horizonte, MG, Brazil. Electronic address: mbeatriz@ufmg.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25466016

Citation

Paiva, Caroline Liboreiro, et al. "Bioactive Amines in Sorghum: Method Optimisation and Influence of Line, Tannin and Hydric Stress." Food Chemistry, vol. 173, 2015, pp. 224-30.
Paiva CL, Evangelista WP, Queiroz VA, et al. Bioactive amines in sorghum: method optimisation and influence of line, tannin and hydric stress. Food Chem. 2015;173:224-30.
Paiva, C. L., Evangelista, W. P., Queiroz, V. A., & Glória, M. B. (2015). Bioactive amines in sorghum: method optimisation and influence of line, tannin and hydric stress. Food Chemistry, 173, 224-30. https://doi.org/10.1016/j.foodchem.2014.10.039
Paiva CL, et al. Bioactive Amines in Sorghum: Method Optimisation and Influence of Line, Tannin and Hydric Stress. Food Chem. 2015 Apr 15;173:224-30. PubMed PMID: 25466016.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioactive amines in sorghum: method optimisation and influence of line, tannin and hydric stress. AU - Paiva,Caroline Liboreiro, AU - Evangelista,Warlley Pinheiro, AU - Queiroz,Valéria Aparecida Vieira, AU - Glória,Maria Beatriz Abreu, Y1 - 2014/10/14/ PY - 2014/07/05/received PY - 2014/10/01/revised PY - 2014/10/07/accepted PY - 2014/12/4/entrez PY - 2014/12/4/pubmed PY - 2015/8/8/medline KW - Agmatine (PubChem CID: 2794990) KW - Bioactive amines KW - Cadaverine (PubChem CID: 80282) KW - Extraction KW - Histamine (PubChem CID: 5818) KW - Hydric stress KW - Phenylethylamine (PubChem CID: 9075) KW - Polyamines KW - Putrescine (PubChem CID: 9532) KW - Serotonin (PubChem CID: 160436) KW - Sorghum bicolor L. KW - Spermidine (PubChem CID: 9539) KW - Spermine (PubChem CID: 9384) KW - Tryptamine (PubChem CID: 67652) KW - Tyramine (PubChem CID: 66449) SP - 224 EP - 30 JF - Food chemistry JO - Food Chem VL - 173 N2 - The profile and levels of bioactive amines in different sorghum lines were reported for the first time. The amines were quantified by ion-pair HPLC, post-column derivatisation with o-phthalaldehyde and fluorimetric detection. The extraction procedure was optimised: 420 μm particle size, extraction with 5% trichloroacetic acid and three extractions. The screening of 22 sorghum lines showed that four of the ten amines investigated were detected. Spermine and spermidine were the prevalent amines (100%), followed by putrescine (77%) and cadaverine (14%). Total amines ranged from 5.8 to 41.4 mg/100 g, and the polyamines represented 60-100% of the total. Sorghum without tannin had higher amines levels compared to sorghum with tannin and cadaverine was specific to samples without tannin. Hydric stress caused accumulation of spermidine in the grains and affected the levels of other amines at rates depending on the presence or not of tannin. Sorghum is a significant source of polyamines. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/25466016/Bioactive_amines_in_sorghum:_method_optimisation_and_influence_of_line_tannin_and_hydric_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(14)01601-X DB - PRIME DP - Unbound Medicine ER -