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Presynaptic alpha 2-adrenoceptor modulation of 5-hydroxytryptamine and noradrenaline release from vascular adrenergic nerves.
Eur J Pharmacol. 1989 May 02; 164(1):35-43.EJ

Abstract

Modulation of the stimulation-evoked release of 5-hydroxytryptamine (5-HT) and noradrenaline (NA) by presynaptic alpha 2-adrenoceptors was characterized in the perfused mesenteric vascular bed of the rat. The vasoconstrictor response to periarterial nerve stimulation (PNS; 8 Hz), previously abolished in the presence of 30 nM prazosin, was restored after 15 min treatment with 10 microM 5-HT, without a significant effect on the pressor response to 1 nmol of infused NA, which was previously abolished with prazosin. The restored pressor response to PNS was abolished by 100 nM tetrodotoxin and 100 nM ketanserin. Clonidine (1-10 microM) in the presence of prazosin induced a dose-dependent potentiation of the restored pressor response to PNS after 5-HT treatment while BHT 920 (10 nM-1 microM) and 100 nM clonidine inhibited the restored response. In the presence of 100 nM phentolamine, the restored pressor response to PNS was not altered by clonidine, but was inhibited by BHT 920. The PNS (8 Hz)-evoked tritium release in a preparation labeled with [3H]5-HT was facilitated by clonidine (100 nM-10 microM) while BHT 920 (10 nM-1 microM) and cocaine (1-10 microM) reduced the release. Yohimbine (1 microM) antagonized the effects of clonidine and cocaine but not of BHT 920 on the PNS-evoked tritium release. In the preparation labeled with [3H]NA, clonidine did not alter the PNS-evoked tritium release while BHT 920 inhibited it and cocaine facilitated it. Yohimbine did not antagonize the effect of BHT 920.(ABSTRACT TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Pharmacology, Miyazaki Medical College, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2546786

Citation

Kawasaki, H, et al. "Presynaptic Alpha 2-adrenoceptor Modulation of 5-hydroxytryptamine and Noradrenaline Release From Vascular Adrenergic Nerves." European Journal of Pharmacology, vol. 164, no. 1, 1989, pp. 35-43.
Kawasaki H, Urabe M, Takasaki K. Presynaptic alpha 2-adrenoceptor modulation of 5-hydroxytryptamine and noradrenaline release from vascular adrenergic nerves. Eur J Pharmacol. 1989;164(1):35-43.
Kawasaki, H., Urabe, M., & Takasaki, K. (1989). Presynaptic alpha 2-adrenoceptor modulation of 5-hydroxytryptamine and noradrenaline release from vascular adrenergic nerves. European Journal of Pharmacology, 164(1), 35-43.
Kawasaki H, Urabe M, Takasaki K. Presynaptic Alpha 2-adrenoceptor Modulation of 5-hydroxytryptamine and Noradrenaline Release From Vascular Adrenergic Nerves. Eur J Pharmacol. 1989 May 2;164(1):35-43. PubMed PMID: 2546786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Presynaptic alpha 2-adrenoceptor modulation of 5-hydroxytryptamine and noradrenaline release from vascular adrenergic nerves. AU - Kawasaki,H, AU - Urabe,M, AU - Takasaki,K, PY - 1989/5/2/pubmed PY - 1989/5/2/medline PY - 1989/5/2/entrez SP - 35 EP - 43 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 164 IS - 1 N2 - Modulation of the stimulation-evoked release of 5-hydroxytryptamine (5-HT) and noradrenaline (NA) by presynaptic alpha 2-adrenoceptors was characterized in the perfused mesenteric vascular bed of the rat. The vasoconstrictor response to periarterial nerve stimulation (PNS; 8 Hz), previously abolished in the presence of 30 nM prazosin, was restored after 15 min treatment with 10 microM 5-HT, without a significant effect on the pressor response to 1 nmol of infused NA, which was previously abolished with prazosin. The restored pressor response to PNS was abolished by 100 nM tetrodotoxin and 100 nM ketanserin. Clonidine (1-10 microM) in the presence of prazosin induced a dose-dependent potentiation of the restored pressor response to PNS after 5-HT treatment while BHT 920 (10 nM-1 microM) and 100 nM clonidine inhibited the restored response. In the presence of 100 nM phentolamine, the restored pressor response to PNS was not altered by clonidine, but was inhibited by BHT 920. The PNS (8 Hz)-evoked tritium release in a preparation labeled with [3H]5-HT was facilitated by clonidine (100 nM-10 microM) while BHT 920 (10 nM-1 microM) and cocaine (1-10 microM) reduced the release. Yohimbine (1 microM) antagonized the effects of clonidine and cocaine but not of BHT 920 on the PNS-evoked tritium release. In the preparation labeled with [3H]NA, clonidine did not alter the PNS-evoked tritium release while BHT 920 inhibited it and cocaine facilitated it. Yohimbine did not antagonize the effect of BHT 920.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/2546786/Presynaptic_alpha_2_adrenoceptor_modulation_of_5_hydroxytryptamine_and_noradrenaline_release_from_vascular_adrenergic_nerves_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0014-2999(89)90228-8 DB - PRIME DP - Unbound Medicine ER -