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Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary.
Ticks Tick Borne Dis. 2015 Mar; 6(2):111-6.TT

Abstract

The aim of this study was to investigate the natural cycle of the new human pathogenic bacteria Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in Southern Hungary. We collected rodents with live-traps (2010-2013) and questing ticks with flagging in 2012. Small mammals were euthanized, tissue samples were collected and all the ectoparasites were removed and stored in 70% alcohol. We found relatively low overall prevalence of tick infestation (8%). Samples were analysed for A. phagocytophilum and Candidatus N. mikurensis with multiplex quantitative real-time PCR targeting a part of major surface protein 2 (msp2) and the heat shock protein groEL genes, respectively. The overall prevalence in tissue samples was 6.6% (skin) and 5.1% (spleen) for A. phagocytophilum and 1.7% (skin) and 3.4% (spleen) for Candidatus N. mikurensis. Candidatus N. mikurensis was only detected in Apodemus flavicollis and Apodemus agrarius, while A. phagocytophilum was found in A. flavicollis, A. agrarius, Myodes glareolus, Microtus arvalis and Mus musculus samples. Prevalence of A. phagocytophilum in skin samples of A. flavicollis was significantly higher than prevalence of N. mikurensis (p<0.05). Among questing Ixodes ricinus ticks we found three (8.8%) individuals (female, male, nymph) infected with Candidatus N. mikurensis. Five (3.1%) questing ticks had A. phagocytophilum infection (one I. ricinus male, two Dermacentor reticulatus females and two Haemaphysalis concinna females). We found one I. ricinus nymph removed from a male A. flavicollis with A. phagocytophilum infection. Our study provides new data on the occurrence of these pathogens in rodent tissue samples, questing ticks and engorged ticks in Southern Hungary.

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Authors+Show Affiliations

Szekeres S
Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent István University, 2 István Street H-1078, Budapest, Hungary. Electronic address: sanyi.szekeres@gmail.com.
Claudia Coipan E
Laboratory for Zoonoses and Environmental Microbiology, National Institute for Public Health and Environment, 9 Antonie van Leeuwenhoeklaan, P.O. Box 1, Bilthoven, The Netherlands. Electronic address: claudia.coipan@rivm.nl.
Rigó K
Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent István University, 2 István Street H-1078, Budapest, Hungary. Electronic address: krigo.mail@gmail.com.
Majoros G
Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent István University, 2 István Street H-1078, Budapest, Hungary. Electronic address: majoros.gabor@aotk.szie.hu.
Jahfari S
Laboratory for Zoonoses and Environmental Microbiology, National Institute for Public Health and Environment, 9 Antonie van Leeuwenhoeklaan, P.O. Box 1, Bilthoven, The Netherlands. Electronic address: seta.jahfari@rivm.nl.
Sprong H
Laboratory for Zoonoses and Environmental Microbiology, National Institute for Public Health and Environment, 9 Antonie van Leeuwenhoeklaan, P.O. Box 1, Bilthoven, The Netherlands. Electronic address: hein.sprong@rivm.nl.
Földvári G
Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent István University, 2 István Street H-1078, Budapest, Hungary. Electronic address: foldvarigabor@gmx.de.

MeSH

Anaplasma phagocytophilumAnaplasmataceaeAnaplasmataceae InfectionsAnimalsArachnid VectorsDNA, BacterialEhrlichiosisFemaleHumansHungaryIxodidaeMalePrevalenceRodentia

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25468763

Citation

Szekeres, Sándor, et al. "Candidatus Neoehrlichia Mikurensis and Anaplasma Phagocytophilum in Natural Rodent and Tick Communities in Southern Hungary." Ticks and Tick-borne Diseases, vol. 6, no. 2, 2015, pp. 111-6.
Szekeres S, Claudia Coipan E, Rigó K, et al. Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary. Ticks Tick Borne Dis. 2015;6(2):111-6.
Szekeres, S., Claudia Coipan, E., Rigó, K., Majoros, G., Jahfari, S., Sprong, H., & Földvári, G. (2015). Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary. Ticks and Tick-borne Diseases, 6(2), 111-6. https://doi.org/10.1016/j.ttbdis.2014.10.004
Szekeres S, et al. Candidatus Neoehrlichia Mikurensis and Anaplasma Phagocytophilum in Natural Rodent and Tick Communities in Southern Hungary. Ticks Tick Borne Dis. 2015;6(2):111-6. PubMed PMID: 25468763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in natural rodent and tick communities in Southern Hungary. AU - Szekeres,Sándor, AU - Claudia Coipan,Elena, AU - Rigó,Krisztina, AU - Majoros,Gábor, AU - Jahfari,Setareh, AU - Sprong,Hein, AU - Földvári,Gábor, Y1 - 2014/11/14/ PY - 2014/05/28/received PY - 2014/09/22/revised PY - 2014/10/27/accepted PY - 2014/12/4/entrez PY - 2014/12/4/pubmed PY - 2016/5/3/medline KW - Anaplasma phagocytophilum KW - Candidatus Neoehrlichia mikurensis KW - Hungary KW - Prevalence KW - Rodent KW - Tick SP - 111 EP - 6 JF - Ticks and tick-borne diseases JO - Ticks Tick Borne Dis VL - 6 IS - 2 N2 - The aim of this study was to investigate the natural cycle of the new human pathogenic bacteria Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum in Southern Hungary. We collected rodents with live-traps (2010-2013) and questing ticks with flagging in 2012. Small mammals were euthanized, tissue samples were collected and all the ectoparasites were removed and stored in 70% alcohol. We found relatively low overall prevalence of tick infestation (8%). Samples were analysed for A. phagocytophilum and Candidatus N. mikurensis with multiplex quantitative real-time PCR targeting a part of major surface protein 2 (msp2) and the heat shock protein groEL genes, respectively. The overall prevalence in tissue samples was 6.6% (skin) and 5.1% (spleen) for A. phagocytophilum and 1.7% (skin) and 3.4% (spleen) for Candidatus N. mikurensis. Candidatus N. mikurensis was only detected in Apodemus flavicollis and Apodemus agrarius, while A. phagocytophilum was found in A. flavicollis, A. agrarius, Myodes glareolus, Microtus arvalis and Mus musculus samples. Prevalence of A. phagocytophilum in skin samples of A. flavicollis was significantly higher than prevalence of N. mikurensis (p<0.05). Among questing Ixodes ricinus ticks we found three (8.8%) individuals (female, male, nymph) infected with Candidatus N. mikurensis. Five (3.1%) questing ticks had A. phagocytophilum infection (one I. ricinus male, two Dermacentor reticulatus females and two Haemaphysalis concinna females). We found one I. ricinus nymph removed from a male A. flavicollis with A. phagocytophilum infection. Our study provides new data on the occurrence of these pathogens in rodent tissue samples, questing ticks and engorged ticks in Southern Hungary. SN - 1877-9603 UR - https://www.unboundmedicine.com/medline/citation/25468763/Candidatus_Neoehrlichia_mikurensis_and_Anaplasma_phagocytophilum_in_natural_rodent_and_tick_communities_in_Southern_Hungary_ DB - PRIME DP - Unbound Medicine ER -