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[Characteristic fingerprint analysis and determination of main components on Andrographis paniculata extract by UPLC].
Zhong Yao Cai. 2014 Jun; 37(6):1055-8.ZY

Abstract

OBJECTIVE

To establish an analytical method for characteristic fingerprint and determination of main components of Andrographis paniculata Extract by UPLC.

METHODS

The chromatographic conditions were Waters ACQUITY UPLC BEH-C18 (2. 1 mm x 0 mm,1.7 μm)by gradient elute using acetonitrile-water as mobile phase(0 -2 min,20% ~ 25% A;2 ~ 5 min,25% ~ 35% A;5 ~ 7 min,35% A;7 ~10 min,35%~ 55% A) at a flow rate of 0. 5 min/mL,detecting wavelength at 220 nm. Results:Contents of the andrographolide, neoandrographolide, 14-deoxyandrographolide and 14-deoxy-l11,12-didehydroandrographolide had good resolution with the correlation coefficients exceed 0. 9999 and the average percent recovery lied in 97. 2% to 103.9%, RSD was less than 3.0% (n = 6). The chromatograms of Andrographis paniculata Extract shared seven common peaks in which four of them were recognized by reference standard with the similarities over 0. 9.

CONCLUSION

It is a fast,accurate and validated method,and can be useful in quality evaluations of Andrographis paniculata Extract.

Authors

Zhang HY
No affiliation info available
Xu XF
No affiliation info available
Huang Y
No affiliation info available
Wamg DQ
No affiliation info available
Deng QH
No affiliation info available

MeSH

AndrographisChromatography, High Pressure LiquidDiterpenesGlucosidesTetrahydronaphthalenes

Pub Type(s)

Journal Article

Language

chi

PubMed ID

25470974

Citation

Zhang, Hui-ye, et al. "[Characteristic Fingerprint Analysis and Determination of Main Components On Andrographis Paniculata Extract By UPLC]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 37, no. 6, 2014, pp. 1055-8.
Zhang HY, Xu XF, Huang Y, et al. [Characteristic fingerprint analysis and determination of main components on Andrographis paniculata extract by UPLC]. Zhong Yao Cai. 2014;37(6):1055-8.
Zhang, H. Y., Xu, X. F., Huang, Y., Wamg, D. Q., & Deng, Q. H. (2014). [Characteristic fingerprint analysis and determination of main components on Andrographis paniculata extract by UPLC]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 37(6), 1055-8.
Zhang HY, et al. [Characteristic Fingerprint Analysis and Determination of Main Components On Andrographis Paniculata Extract By UPLC]. Zhong Yao Cai. 2014;37(6):1055-8. PubMed PMID: 25470974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Characteristic fingerprint analysis and determination of main components on Andrographis paniculata extract by UPLC]. AU - Zhang,Hui-ye, AU - Xu,Xiao-fei, AU - Huang,Yong, AU - Wamg,De-qin, AU - Deng,Qiao-hua, PY - 2014/12/5/entrez PY - 2014/12/5/pubmed PY - 2015/7/18/medline SP - 1055 EP - 8 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 37 IS - 6 N2 - OBJECTIVE: To establish an analytical method for characteristic fingerprint and determination of main components of Andrographis paniculata Extract by UPLC. METHODS: The chromatographic conditions were Waters ACQUITY UPLC BEH-C18 (2. 1 mm x 0 mm,1.7 μm)by gradient elute using acetonitrile-water as mobile phase(0 -2 min,20% ~ 25% A;2 ~ 5 min,25% ~ 35% A;5 ~ 7 min,35% A;7 ~10 min,35%~ 55% A) at a flow rate of 0. 5 min/mL,detecting wavelength at 220 nm. Results:Contents of the andrographolide, neoandrographolide, 14-deoxyandrographolide and 14-deoxy-l11,12-didehydroandrographolide had good resolution with the correlation coefficients exceed 0. 9999 and the average percent recovery lied in 97. 2% to 103.9%, RSD was less than 3.0% (n = 6). The chromatograms of Andrographis paniculata Extract shared seven common peaks in which four of them were recognized by reference standard with the similarities over 0. 9. CONCLUSION: It is a fast,accurate and validated method,and can be useful in quality evaluations of Andrographis paniculata Extract. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/25470974/[Characteristic_fingerprint_analysis_and_determination_of_main_components_on_Andrographis_paniculata_extract_by_UPLC]_ DB - PRIME DP - Unbound Medicine ER -