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Leishmanicidal and cytotoxic activities of Nigella sativa and its active principle, thymoquinone.
Pharm Biol. 2015 Jul; 53(7):1052-7.PB

Abstract

CONTEXT

Leishmaniasis is a complex disease with a broad spectrum of clinical presentations.

OBJECTIVE

We evaluated the anti-leishmanial effects of Nigella sativa L. (Ranunculaceae) against Leishmania tropica and Leishmania infantum with an in vitro model.

MATERIALS AND METHODS

Antileishmanial effects of essential oil and methanolic extract of N. sativa (0-200 µg/mL) and thymoquinone (0-25 µg/mL) on promastigotes of both species and their cytotoxicity activities against murine macrophages were evaluated using the MTT assay at 24, 48, and 72 h. Moreover, their leishmanicidal effects against amastigotes were investigated in a macrophage model, for 48 and 72 h.

RESULTS

The findings showed that essential oil (L. tropica IC50 9.3 μg/mL and L. infantum IC50 11.7 μg/mL) and methanolic extract (L. tropica IC50 14.8 μg/mL and L. infantum IC50 15.7 μg/mL) of N. sativa, particularly thymoquinone (L. tropica IC50 1.16 μg/mL and L. infantum IC50 1.47 μg/mL), had potent antileishmanial activity on promastigotes of both species after 72 h. In addition, essential oil (L. tropica IC50 21.4 μg/mL and L. infantum IC50 26.3 μg/mL), methanolic extract (L. tropica IC50 30.8 μg/mL and L. infantum IC50 34.6 μg/mL), and thymoquinone (L. tropica IC50 2.1 μg/mL and L. infantum IC50 2.6 μg/mL) mediated a significant decrease in the growth rate of amastigote forms of both species. Thymoquinone (CC50 38.8 μg/mL) exhibited higher cytotoxic effects against murine macrophages than the other extracts.

CONCLUSION

N. sativa, especially its active principle, thymoquinone, showed a potent leishmanicidal activity against L. tropica and L.infantum with an in vitro model.

Authors+Show Affiliations

Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences , Kerman , Iran .No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25471014

Citation

Mahmoudvand, Hossein, et al. "Leishmanicidal and Cytotoxic Activities of Nigella Sativa and Its Active Principle, Thymoquinone." Pharmaceutical Biology, vol. 53, no. 7, 2015, pp. 1052-7.
Mahmoudvand H, Tavakoli R, Sharififar F, et al. Leishmanicidal and cytotoxic activities of Nigella sativa and its active principle, thymoquinone. Pharm Biol. 2015;53(7):1052-7.
Mahmoudvand, H., Tavakoli, R., Sharififar, F., Minaie, K., Ezatpour, B., Jahanbakhsh, S., & Sharifi, I. (2015). Leishmanicidal and cytotoxic activities of Nigella sativa and its active principle, thymoquinone. Pharmaceutical Biology, 53(7), 1052-7. https://doi.org/10.3109/13880209.2014.957784
Mahmoudvand H, et al. Leishmanicidal and Cytotoxic Activities of Nigella Sativa and Its Active Principle, Thymoquinone. Pharm Biol. 2015;53(7):1052-7. PubMed PMID: 25471014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leishmanicidal and cytotoxic activities of Nigella sativa and its active principle, thymoquinone. AU - Mahmoudvand,Hossein, AU - Tavakoli,Razieh, AU - Sharififar,Fariba, AU - Minaie,Keyhan, AU - Ezatpour,Behrouz, AU - Jahanbakhsh,Sareh, AU - Sharifi,Iraj, Y1 - 2014/12/04/ PY - 2014/12/5/entrez PY - 2014/12/5/pubmed PY - 2016/3/2/medline KW - Black cumin KW - Leishmania infantum KW - Leishmania tropica SP - 1052 EP - 7 JF - Pharmaceutical biology JO - Pharm Biol VL - 53 IS - 7 N2 - CONTEXT: Leishmaniasis is a complex disease with a broad spectrum of clinical presentations. OBJECTIVE: We evaluated the anti-leishmanial effects of Nigella sativa L. (Ranunculaceae) against Leishmania tropica and Leishmania infantum with an in vitro model. MATERIALS AND METHODS: Antileishmanial effects of essential oil and methanolic extract of N. sativa (0-200 µg/mL) and thymoquinone (0-25 µg/mL) on promastigotes of both species and their cytotoxicity activities against murine macrophages were evaluated using the MTT assay at 24, 48, and 72 h. Moreover, their leishmanicidal effects against amastigotes were investigated in a macrophage model, for 48 and 72 h. RESULTS: The findings showed that essential oil (L. tropica IC50 9.3 μg/mL and L. infantum IC50 11.7 μg/mL) and methanolic extract (L. tropica IC50 14.8 μg/mL and L. infantum IC50 15.7 μg/mL) of N. sativa, particularly thymoquinone (L. tropica IC50 1.16 μg/mL and L. infantum IC50 1.47 μg/mL), had potent antileishmanial activity on promastigotes of both species after 72 h. In addition, essential oil (L. tropica IC50 21.4 μg/mL and L. infantum IC50 26.3 μg/mL), methanolic extract (L. tropica IC50 30.8 μg/mL and L. infantum IC50 34.6 μg/mL), and thymoquinone (L. tropica IC50 2.1 μg/mL and L. infantum IC50 2.6 μg/mL) mediated a significant decrease in the growth rate of amastigote forms of both species. Thymoquinone (CC50 38.8 μg/mL) exhibited higher cytotoxic effects against murine macrophages than the other extracts. CONCLUSION: N. sativa, especially its active principle, thymoquinone, showed a potent leishmanicidal activity against L. tropica and L.infantum with an in vitro model. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25471014/Leishmanicidal_and_cytotoxic_activities_of_Nigella_sativa_and_its_active_principle_thymoquinone_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2014.957784 DB - PRIME DP - Unbound Medicine ER -