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Genetic and physical mapping of powdery mildew resistance gene MlHLT in Chinese wheat landrace Hulutou.
Theor Appl Genet. 2015 Feb; 128(2):365-73.TA

Abstract

KEY MESSAGE

A powdery mildew resistance gene MlHLT derived from a Chinese wheat landrace maps within a 3.6 centimorgan (cM) genetic interval spanning a 13.4 megabase (Mb) physical genomic region on chromosome 1DS. Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt) is a devastating disease that can cause severe yield losses. Chinese wheat landrace Hulutou confers nearly immune resistance against prevailing Bgt isolate E09 in Beijing. Genetic analysis indicate that the powdery mildew resistance of Hulutou is controlled by a single dominant gene, provisionally designated MlHLT. Bulked segregant analysis(BSA) and simple sequence repeat (SSR) mapping showed that MlHLT is located on chromosome arm 1DS between markers Xgwm337 and Xcfd83/Xcfd72. By applying comparative genomics analysis, collinearity genomic regions of the MlHLT locus on Aegilops tauschii chromosome 1DS were identified in Brachypodium distachyon chromosome 2, rice chromosome 5 and sorghum chromosome 9, respectively. Three new polymorphic markers were developed using the draft genome sequences and the extended single nucleotide polymorphism (SNP) marker sequences of Ae. tauschii accession AL8/78, as well as the Triticum aestivum cv. Chinese Spring 454 contig sequences and the International Wheat Genome Sequencing Consortium (IWGSC) survey sequences. MlHLT mapped into a 3.6 cM genetic interval spanning 13.4 Mb physical genomic region containing seven contigs (ctg220, ctg4623, ctg1063, ctg5929, ctg3163, ctg699 and ctg1065) on 1DS that has synteny with a 369.8 kb genomic region in Brachypodium, a 380.8 kb genomic region in rice and a 298.4 kb genomic region in sorghum. The genetic and physical maps of MlHLT provide framework for map-based cloning and marker-assisted selection (MAS) of the powdery mildew resistance gene MlHLT in Hulutou.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang Z
Department of Plant Genetics and Breeding/State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, China.
Li H
No affiliation info available
Zhang D
No affiliation info available
Guo L
No affiliation info available
Chen J
No affiliation info available
Chen Y
No affiliation info available
Wu Q
No affiliation info available
Xie J
No affiliation info available
Zhang Y
No affiliation info available
Sun Q
No affiliation info available
Dvorak J
No affiliation info available
Luo MC
No affiliation info available
Liu Z
No affiliation info available

MeSH

AscomycotaChromosomes, PlantComparative Genomic HybridizationDNA, PlantDisease ResistanceExpressed Sequence TagsGenes, PlantGenetic MarkersMicrosatellite RepeatsPhysical Chromosome MappingPlant DiseasesPolymorphism, Single NucleotideTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25471672

Citation

Wang, Zhenzhong, et al. "Genetic and Physical Mapping of Powdery Mildew Resistance Gene MlHLT in Chinese Wheat Landrace Hulutou." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 128, no. 2, 2015, pp. 365-73.
Wang Z, Li H, Zhang D, et al. Genetic and physical mapping of powdery mildew resistance gene MlHLT in Chinese wheat landrace Hulutou. Theor Appl Genet. 2015;128(2):365-73.
Wang, Z., Li, H., Zhang, D., Guo, L., Chen, J., Chen, Y., Wu, Q., Xie, J., Zhang, Y., Sun, Q., Dvorak, J., Luo, M. C., & Liu, Z. (2015). Genetic and physical mapping of powdery mildew resistance gene MlHLT in Chinese wheat landrace Hulutou. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 128(2), 365-73. https://doi.org/10.1007/s00122-014-2436-2
Wang Z, et al. Genetic and Physical Mapping of Powdery Mildew Resistance Gene MlHLT in Chinese Wheat Landrace Hulutou. Theor Appl Genet. 2015;128(2):365-73. PubMed PMID: 25471672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic and physical mapping of powdery mildew resistance gene MlHLT in Chinese wheat landrace Hulutou. AU - Wang,Zhenzhong, AU - Li,Hanwen, AU - Zhang,Deyun, AU - Guo,Li, AU - Chen,Jiaojiao, AU - Chen,Yongxing, AU - Wu,Qiuhong, AU - Xie,Jingzhong, AU - Zhang,Yan, AU - Sun,Qixin, AU - Dvorak,Jan, AU - Luo,Ming-Cheng, AU - Liu,Zhiyong, Y1 - 2014/12/04/ PY - 2014/08/14/received PY - 2014/11/21/accepted PY - 2014/12/5/entrez PY - 2014/12/5/pubmed PY - 2015/6/25/medline SP - 365 EP - 73 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 128 IS - 2 N2 - KEY MESSAGE: A powdery mildew resistance gene MlHLT derived from a Chinese wheat landrace maps within a 3.6 centimorgan (cM) genetic interval spanning a 13.4 megabase (Mb) physical genomic region on chromosome 1DS. Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt) is a devastating disease that can cause severe yield losses. Chinese wheat landrace Hulutou confers nearly immune resistance against prevailing Bgt isolate E09 in Beijing. Genetic analysis indicate that the powdery mildew resistance of Hulutou is controlled by a single dominant gene, provisionally designated MlHLT. Bulked segregant analysis(BSA) and simple sequence repeat (SSR) mapping showed that MlHLT is located on chromosome arm 1DS between markers Xgwm337 and Xcfd83/Xcfd72. By applying comparative genomics analysis, collinearity genomic regions of the MlHLT locus on Aegilops tauschii chromosome 1DS were identified in Brachypodium distachyon chromosome 2, rice chromosome 5 and sorghum chromosome 9, respectively. Three new polymorphic markers were developed using the draft genome sequences and the extended single nucleotide polymorphism (SNP) marker sequences of Ae. tauschii accession AL8/78, as well as the Triticum aestivum cv. Chinese Spring 454 contig sequences and the International Wheat Genome Sequencing Consortium (IWGSC) survey sequences. MlHLT mapped into a 3.6 cM genetic interval spanning 13.4 Mb physical genomic region containing seven contigs (ctg220, ctg4623, ctg1063, ctg5929, ctg3163, ctg699 and ctg1065) on 1DS that has synteny with a 369.8 kb genomic region in Brachypodium, a 380.8 kb genomic region in rice and a 298.4 kb genomic region in sorghum. The genetic and physical maps of MlHLT provide framework for map-based cloning and marker-assisted selection (MAS) of the powdery mildew resistance gene MlHLT in Hulutou. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/25471672/Genetic_and_physical_mapping_of_powdery_mildew_resistance_gene_MlHLT_in_Chinese_wheat_landrace_Hulutou_ DB - PRIME DP - Unbound Medicine ER -