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Hepatoprotective effects of Portulaca oleracea extract against CCl4-induced damage in rats.
Pharm Biol. 2015 Jul; 53(7):1042-51.PB

Abstract

CONTEXT

Purslane (Portulaca oleracea L., Portulacaceae) has been traditionally used in folk medicine to afford protection against liver injury, although its actual efficacy remains uncertain.

OBJECTIVE

To evaluate purslane as a hepatoprotective agent, we investigated the protective effect of its ethanol extract against carbon tetrachloride (CCl4)-induced hepatic toxicity in rats.

MATERIALS AND METHODS

A total of 108 male Wistar rats were randomly divided into 12 groups. The first group was maintained as normal control, whereas CCl4 (0.5 ml/kg bw, 50% CCl4 in olive oil, i.p.), purslane extract (0.005, 0.01, 0.05, 0.1, and 0.15 g/kg bw, intragastrically), and purslane extract (five doses as above) along with CCl4 were administered to the Groups II, III-VII, and VIII-XII, respectively. The rats were sacrificed on the 30th day, and blood was withdrawn by cardiac puncture. Liver damage was assessed by measuring hepatic marker enzymes (ALT, AST, ALP, GGT, and SOD) and histopathological observation.

RESULTS

Treatment with CCl4 resulted in increased serum activities of marker enzymes with a concomitant decrease in SOD. Histological alterations were also observed in the liver tissue upon CCl4 treatment. Administration of purslane extract (0.01, 0.05, 0.1, and 0.15 g/kg b.w.) significantly showed a marked tendency towards normalization of all measured biochemical parameters in CCl4-treated rats. Histopathological changes also paralleled the detected alteration in markers of liver function.

DISCUSSION AND CONCLUSION

These results demonstrate that purslane exerts protective effects against CCl4-induced damage in rat liver and supports a potential therapeutic use of purslane as an alternative for patients with liver diseases.

Authors+Show Affiliations

Department of Biology, Science and Research Branch, Islamic Azad University , Tehran , Iran .No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25472695

Citation

Eidi, Akram, et al. "Hepatoprotective Effects of Portulaca Oleracea Extract Against CCl4-induced Damage in Rats." Pharmaceutical Biology, vol. 53, no. 7, 2015, pp. 1042-51.
Eidi A, Mortazavi P, Moghadam JZ, et al. Hepatoprotective effects of Portulaca oleracea extract against CCl4-induced damage in rats. Pharm Biol. 2015;53(7):1042-51.
Eidi, A., Mortazavi, P., Moghadam, J. Z., & Mardani, P. M. (2015). Hepatoprotective effects of Portulaca oleracea extract against CCl4-induced damage in rats. Pharmaceutical Biology, 53(7), 1042-51. https://doi.org/10.3109/13880209.2014.957783
Eidi A, et al. Hepatoprotective Effects of Portulaca Oleracea Extract Against CCl4-induced Damage in Rats. Pharm Biol. 2015;53(7):1042-51. PubMed PMID: 25472695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective effects of Portulaca oleracea extract against CCl4-induced damage in rats. AU - Eidi,Akram, AU - Mortazavi,Pejman, AU - Moghadam,Jalal Zarringhalam, AU - Mardani,Parisa Mousavi, Y1 - 2014/12/04/ PY - 2014/12/5/entrez PY - 2014/12/5/pubmed PY - 2016/3/2/medline KW - Carbon tetrachloride KW - detoxification KW - liver KW - purslane SP - 1042 EP - 51 JF - Pharmaceutical biology JO - Pharm Biol VL - 53 IS - 7 N2 - CONTEXT: Purslane (Portulaca oleracea L., Portulacaceae) has been traditionally used in folk medicine to afford protection against liver injury, although its actual efficacy remains uncertain. OBJECTIVE: To evaluate purslane as a hepatoprotective agent, we investigated the protective effect of its ethanol extract against carbon tetrachloride (CCl4)-induced hepatic toxicity in rats. MATERIALS AND METHODS: A total of 108 male Wistar rats were randomly divided into 12 groups. The first group was maintained as normal control, whereas CCl4 (0.5 ml/kg bw, 50% CCl4 in olive oil, i.p.), purslane extract (0.005, 0.01, 0.05, 0.1, and 0.15 g/kg bw, intragastrically), and purslane extract (five doses as above) along with CCl4 were administered to the Groups II, III-VII, and VIII-XII, respectively. The rats were sacrificed on the 30th day, and blood was withdrawn by cardiac puncture. Liver damage was assessed by measuring hepatic marker enzymes (ALT, AST, ALP, GGT, and SOD) and histopathological observation. RESULTS: Treatment with CCl4 resulted in increased serum activities of marker enzymes with a concomitant decrease in SOD. Histological alterations were also observed in the liver tissue upon CCl4 treatment. Administration of purslane extract (0.01, 0.05, 0.1, and 0.15 g/kg b.w.) significantly showed a marked tendency towards normalization of all measured biochemical parameters in CCl4-treated rats. Histopathological changes also paralleled the detected alteration in markers of liver function. DISCUSSION AND CONCLUSION: These results demonstrate that purslane exerts protective effects against CCl4-induced damage in rat liver and supports a potential therapeutic use of purslane as an alternative for patients with liver diseases. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25472695/Hepatoprotective_effects_of_Portulaca_oleracea_extract_against_CCl4_induced_damage_in_rats_ DB - PRIME DP - Unbound Medicine ER -